Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896562796> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2896562796 endingPage "598" @default.
- W2896562796 startingPage "590" @default.
- W2896562796 abstract "ABSTRACT The transmission of tobamovirus by tomato and pepper seeds is an important mean of virus introduction in crops. Therefore, detecting its presence in the seed becomes essential for the preventive control of virus diseases. In this study, a method was proposed for the detection of Tobacco mosaic virus (TMV) and Tomato mosaic virus (ToMV) in tomatoes (Solanum lycopersicum) and Pepper mild mottle virus (PMMoV) in pepper (Capsicum annum) seeds. Seed lots with different levels of incidence were analyzed by biological, serological, and molecular methods. Using DAS-ELISA technique, it was possible to detect TMV up to the limit rate of 1:170 (1 contaminated seed: total seeds) and the ToMV up to 1:200 in tomato seeds; PMMoV in pepper seeds was detected up to 1:140. The IC-RT-PCR detected the TMV and ToMV up to the limit of 1:400 and PMMoV up to the limit of 1:300. The assembled lots containing only 1 contaminated seed in 1000 (1:1000) were combined into 30 sub lots for DAS-ELISA analysis and 10 sub lots for IC-RT-PCR analysis. Both techniques, DAS-ELISA and IC-RT-PCR, were efficient to detect the three viruses in all analyzed samples, but the detection of tobamoviruses with RT-PCR and biological tests was not reliable. Based on the results of this study, in which a combination of seeds in sub lots was made to reduce the number of tests performed, it is possible to make significant savings in the cost of the diagnostic methods routinely conducted in official laboratories, with high efficiency and reliability." @default.
- W2896562796 created "2018-10-26" @default.
- W2896562796 creator A5014861674 @default.
- W2896562796 creator A5022154117 @default.
- W2896562796 creator A5056560253 @default.
- W2896562796 creator A5073027324 @default.
- W2896562796 creator A5084800764 @default.
- W2896562796 date "2018-10-18" @default.
- W2896562796 modified "2023-09-25" @default.
- W2896562796 title "Procedure for detecting tobamovirus in tomato and pepper seeds decreases the cost analysis" @default.
- W2896562796 cites W1747388711 @default.
- W2896562796 cites W1924991893 @default.
- W2896562796 cites W1970134228 @default.
- W2896562796 cites W1973207608 @default.
- W2896562796 cites W1984403332 @default.
- W2896562796 cites W1991149924 @default.
- W2896562796 cites W2017995658 @default.
- W2896562796 cites W2029194001 @default.
- W2896562796 cites W2045670307 @default.
- W2896562796 cites W2051254871 @default.
- W2896562796 cites W2069544263 @default.
- W2896562796 cites W2070855000 @default.
- W2896562796 cites W2097213912 @default.
- W2896562796 cites W2118081260 @default.
- W2896562796 cites W2119532070 @default.
- W2896562796 cites W2174766121 @default.
- W2896562796 cites W2344071590 @default.
- W2896562796 cites W2403406460 @default.
- W2896562796 cites W262377650 @default.
- W2896562796 cites W303927472 @default.
- W2896562796 doi "https://doi.org/10.1590/1678-4499.2017317" @default.
- W2896562796 hasPublicationYear "2018" @default.
- W2896562796 type Work @default.
- W2896562796 sameAs 2896562796 @default.
- W2896562796 citedByCount "4" @default.
- W2896562796 countsByYear W28965627962021 @default.
- W2896562796 countsByYear W28965627962022 @default.
- W2896562796 countsByYear W28965627962023 @default.
- W2896562796 crossrefType "journal-article" @default.
- W2896562796 hasAuthorship W2896562796A5014861674 @default.
- W2896562796 hasAuthorship W2896562796A5022154117 @default.
- W2896562796 hasAuthorship W2896562796A5056560253 @default.
- W2896562796 hasAuthorship W2896562796A5073027324 @default.
- W2896562796 hasAuthorship W2896562796A5084800764 @default.
- W2896562796 hasBestOaLocation W28965627961 @default.
- W2896562796 hasConcept C109110057 @default.
- W2896562796 hasConcept C144027150 @default.
- W2896562796 hasConcept C144707691 @default.
- W2896562796 hasConcept C159047783 @default.
- W2896562796 hasConcept C2522874641 @default.
- W2896562796 hasConcept C2776242653 @default.
- W2896562796 hasConcept C2778786252 @default.
- W2896562796 hasConcept C2781083041 @default.
- W2896562796 hasConcept C42972112 @default.
- W2896562796 hasConcept C6557445 @default.
- W2896562796 hasConcept C71924100 @default.
- W2896562796 hasConcept C86803240 @default.
- W2896562796 hasConceptScore W2896562796C109110057 @default.
- W2896562796 hasConceptScore W2896562796C144027150 @default.
- W2896562796 hasConceptScore W2896562796C144707691 @default.
- W2896562796 hasConceptScore W2896562796C159047783 @default.
- W2896562796 hasConceptScore W2896562796C2522874641 @default.
- W2896562796 hasConceptScore W2896562796C2776242653 @default.
- W2896562796 hasConceptScore W2896562796C2778786252 @default.
- W2896562796 hasConceptScore W2896562796C2781083041 @default.
- W2896562796 hasConceptScore W2896562796C42972112 @default.
- W2896562796 hasConceptScore W2896562796C6557445 @default.
- W2896562796 hasConceptScore W2896562796C71924100 @default.
- W2896562796 hasConceptScore W2896562796C86803240 @default.
- W2896562796 hasIssue "4" @default.
- W2896562796 hasLocation W28965627961 @default.
- W2896562796 hasLocation W28965627962 @default.
- W2896562796 hasLocation W28965627963 @default.
- W2896562796 hasLocation W28965627964 @default.
- W2896562796 hasOpenAccess W2896562796 @default.
- W2896562796 hasPrimaryLocation W28965627961 @default.
- W2896562796 hasRelatedWork W1973104548 @default.
- W2896562796 hasRelatedWork W1981790803 @default.
- W2896562796 hasRelatedWork W2065604743 @default.
- W2896562796 hasRelatedWork W2084446007 @default.
- W2896562796 hasRelatedWork W2124117763 @default.
- W2896562796 hasRelatedWork W2228711096 @default.
- W2896562796 hasRelatedWork W2348046417 @default.
- W2896562796 hasRelatedWork W2523514503 @default.
- W2896562796 hasRelatedWork W2896562796 @default.
- W2896562796 hasRelatedWork W3173703112 @default.
- W2896562796 hasVolume "77" @default.
- W2896562796 isParatext "false" @default.
- W2896562796 isRetracted "false" @default.
- W2896562796 magId "2896562796" @default.
- W2896562796 workType "article" @default.