Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896579710> ?p ?o ?g. }
- W2896579710 abstract "Abstract Objectives The present study aimed to reveal expression status of the neddylation enzymes in HNSCC and to elucidate the anticancer efficacy and the underlying mechanisms of inhibiting neddylation pathway. Materials and methods The expression levels of neddylation enzymes were estimated by Western blotting in human HNSCC specimens and bioinformatics analysis of the cancer genome atlas ( TCGA ) database. Cell apoptosis was evaluated by Annexin V fluorescein isothiocyanate/propidium iodide (Annexin V‐ FITC / PI ) stain and fluorescence‐activated cell sorting ( FACS ). Small interfering RNA (si RNA ) and the CRISPR ‐Cas9 system were used to elucidate the underlying molecular mechanism of MLN 4924‐induced HNSCC apoptosis. Results Expression levels of NAE 1 and UBC 12 were prominently higher in HNSCC tissues than that in normal tissues. Inactivation of the neddylation pathway significantly inhibited malignant phenotypes of HNSCC cells. Mechanistic studies revealed that MLN 4924 induced the accumulation of CRL ligase substrate c‐Myc that transcriptionally activated pro‐apoptotic protein Noxa, which triggered apoptosis in HNSCC . Conclusions These findings determined the over‐expression levels of neddylation enzymes in HNSCC and revealed novel mechanisms underlying neddylation inhibition induced growth suppression in HNSCC cells, which provided preclinical evidence for further clinical evaluation of neddylation inhibitors (eg, MLN 4924) for the treatment of HNSCC ." @default.
- W2896579710 created "2018-10-26" @default.
- W2896579710 creator A5011072956 @default.
- W2896579710 creator A5012920727 @default.
- W2896579710 creator A5017055102 @default.
- W2896579710 creator A5017851336 @default.
- W2896579710 creator A5019611706 @default.
- W2896579710 creator A5031134200 @default.
- W2896579710 creator A5039708869 @default.
- W2896579710 creator A5052501867 @default.
- W2896579710 creator A5055165608 @default.
- W2896579710 creator A5057388214 @default.
- W2896579710 creator A5058913315 @default.
- W2896579710 creator A5059646032 @default.
- W2896579710 creator A5067011111 @default.
- W2896579710 creator A5068090216 @default.
- W2896579710 creator A5071030446 @default.
- W2896579710 creator A5080974950 @default.
- W2896579710 creator A5085500621 @default.
- W2896579710 date "2018-10-19" @default.
- W2896579710 modified "2023-10-17" @default.
- W2896579710 title "The Nedd8‐activating enzyme inhibitor <scp>MLN</scp>4924 (<scp>TAK</scp>‐924/Pevonedistat) induces apoptosis via c‐Myc‐Noxa axis in head and neck squamous cell carcinoma" @default.
- W2896579710 cites W1504206591 @default.
- W2896579710 cites W1518520618 @default.
- W2896579710 cites W1526473325 @default.
- W2896579710 cites W1536843121 @default.
- W2896579710 cites W1539642496 @default.
- W2896579710 cites W1574166501 @default.
- W2896579710 cites W1598608016 @default.
- W2896579710 cites W1776950904 @default.
- W2896579710 cites W1864563621 @default.
- W2896579710 cites W1966203663 @default.
- W2896579710 cites W1972608147 @default.
- W2896579710 cites W1981989535 @default.
- W2896579710 cites W1983647988 @default.
- W2896579710 cites W1983821491 @default.
- W2896579710 cites W1988031203 @default.
- W2896579710 cites W1989088998 @default.
- W2896579710 cites W1989808176 @default.
- W2896579710 cites W1992676863 @default.
- W2896579710 cites W1997845902 @default.
- W2896579710 cites W1999613518 @default.
- W2896579710 cites W2014114945 @default.
- W2896579710 cites W2014847481 @default.
- W2896579710 cites W2021846401 @default.
- W2896579710 cites W2033940940 @default.
- W2896579710 cites W2040798958 @default.
- W2896579710 cites W2050259155 @default.
- W2896579710 cites W2051887481 @default.
- W2896579710 cites W2053874183 @default.
- W2896579710 cites W2062312459 @default.
- W2896579710 cites W2066239321 @default.
- W2896579710 cites W2069387298 @default.
- W2896579710 cites W2072227049 @default.
- W2896579710 cites W2076194625 @default.
- W2896579710 cites W2083873521 @default.
- W2896579710 cites W2085006244 @default.
- W2896579710 cites W2085664561 @default.
- W2896579710 cites W2095107958 @default.
- W2896579710 cites W2099692341 @default.
- W2896579710 cites W2101700977 @default.
- W2896579710 cites W2110508238 @default.
- W2896579710 cites W2122405410 @default.
- W2896579710 cites W2128546338 @default.
- W2896579710 cites W2132215082 @default.
- W2896579710 cites W2132251348 @default.
- W2896579710 cites W2135788003 @default.
- W2896579710 cites W2136729555 @default.
- W2896579710 cites W2138426721 @default.
- W2896579710 cites W2140494980 @default.
- W2896579710 cites W2146217870 @default.
- W2896579710 cites W2147045066 @default.
- W2896579710 cites W2148532207 @default.
- W2896579710 cites W2148636166 @default.
- W2896579710 cites W2160823702 @default.
- W2896579710 cites W2161933442 @default.
- W2896579710 cites W2168182785 @default.
- W2896579710 cites W2184335538 @default.
- W2896579710 cites W2194749745 @default.
- W2896579710 cites W2297334570 @default.
- W2896579710 cites W2312508287 @default.
- W2896579710 cites W2334281392 @default.
- W2896579710 cites W2343059233 @default.
- W2896579710 cites W2403580382 @default.
- W2896579710 cites W2595030032 @default.
- W2896579710 cites W2620930278 @default.
- W2896579710 cites W2734686848 @default.
- W2896579710 cites W2749328884 @default.
- W2896579710 cites W754635985 @default.
- W2896579710 doi "https://doi.org/10.1111/cpr.12536" @default.
- W2896579710 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6496207" @default.
- W2896579710 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30341788" @default.
- W2896579710 hasPublicationYear "2018" @default.
- W2896579710 type Work @default.
- W2896579710 sameAs 2896579710 @default.
- W2896579710 citedByCount "17" @default.
- W2896579710 countsByYear W28965797102019 @default.
- W2896579710 countsByYear W28965797102020 @default.
- W2896579710 countsByYear W28965797102021 @default.
- W2896579710 countsByYear W28965797102022 @default.