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- W2896583358 abstract "Dynein adaptor proteins such as Bicaudal D2 (BicD2) are integral components of the dynein transport machinery, as they recognize cargoes for cell cycle-specific transport and link them to the motor complex. Human BicD2 switches from selecting secretory and Golgi-derived vesicles for transport in G1 and S phase (by recognizing Rab6GTP), to selecting the nucleus for transport in G2 phase (by recognizing nuclear pore protein Nup358), but the molecular mechanisms governing this switch are elusive. Here, we have developed a quantitative model for BicD2/cargo interactions that integrates affinities, oligomeric states, and cellular concentrations of the reactants. BicD2 and cargo form predominantly 2:2 complexes. Furthermore, the affinity of BicD2 toward its cargo Nup358 is higher than that toward Rab6GTP. Based on our calculations, an estimated 1000 BicD2 molecules per cell would be recruited to the nucleus through Nup358 in the absence of regulation. Notably, RanGTP is a negative regulator of the Nup358/BicD2 interaction that weakens the affinity by a factor of 10 and may play a role in averting dynein recruitment to the nucleus outside of the G2 phase. However, our quantitative model predicts that an additional negative regulator remains to be identified. In the absence of negative regulation, the affinity of Nup358 would likely be sufficient to recruit BicD2 to the nucleus in G2 phase. Our quantitative model makes testable predictions of how cellular transport events are orchestrated. These transport processes are important for brain development, cell cycle control, signaling, and neurotransmission at synapses." @default.
- W2896583358 created "2018-10-26" @default.
- W2896583358 creator A5005725779 @default.
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- W2896583358 date "2018-10-22" @default.
- W2896583358 modified "2023-10-06" @default.
- W2896583358 title "A Quantitative Model for BicD2/Cargo Interactions" @default.
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- W2896583358 cites W1609104027 @default.
- W2896583358 cites W1969477331 @default.
- W2896583358 cites W1974454876 @default.
- W2896583358 cites W1984926639 @default.
- W2896583358 cites W1993233728 @default.
- W2896583358 cites W1997600667 @default.
- W2896583358 cites W2000570159 @default.
- W2896583358 cites W2004524985 @default.
- W2896583358 cites W2011119616 @default.
- W2896583358 cites W2015058678 @default.
- W2896583358 cites W2016958205 @default.
- W2896583358 cites W2017440930 @default.
- W2896583358 cites W2019318168 @default.
- W2896583358 cites W2019928476 @default.
- W2896583358 cites W2024626678 @default.
- W2896583358 cites W2029225125 @default.
- W2896583358 cites W2030053673 @default.
- W2896583358 cites W2031053226 @default.
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- W2896583358 cites W2038570204 @default.
- W2896583358 cites W2047730219 @default.
- W2896583358 cites W2053569747 @default.
- W2896583358 cites W2058905123 @default.
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- W2896583358 cites W2061966099 @default.
- W2896583358 cites W2064295826 @default.
- W2896583358 cites W2065299512 @default.
- W2896583358 cites W2070136863 @default.
- W2896583358 cites W2071884450 @default.
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- W2896583358 cites W2092359152 @default.
- W2896583358 cites W2092360229 @default.
- W2896583358 cites W2099485650 @default.
- W2896583358 cites W2101447312 @default.
- W2896583358 cites W2104389902 @default.
- W2896583358 cites W2107930047 @default.
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- W2896583358 cites W2161809910 @default.
- W2896583358 cites W2163174294 @default.
- W2896583358 cites W2163408061 @default.
- W2896583358 cites W2171061283 @default.
- W2896583358 cites W2220896177 @default.
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- W2896583358 cites W2479788400 @default.
- W2896583358 cites W2588427561 @default.
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- W2896583358 doi "https://doi.org/10.1021/acs.biochem.8b00987" @default.
- W2896583358 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6520106" @default.
- W2896583358 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30345745" @default.
- W2896583358 hasPublicationYear "2018" @default.
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