Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896677307> ?p ?o ?g. }
- W2896677307 abstract "Abstract Kinetochores are macromolecular protein complexes assembled on centromeric chromatin that ensure accurate chromosome segregation by linking DNA to spindle microtubules and integrating safeguard mechanisms. A kinetochore-associated pool of Ipl1 Aurora B kinase, a subunit of the chromosomal passenger complex (CPC), was previously implicated in feedback control mechanisms. To study the kinetochore subunit connectivity built on budding yeast point centromeres and its CPC interactions we performed crosslink-guided in vitro reconstitution. The Ame1/Okp1 CENP-U/Q heterodimer, forming the COMA complex with Ctf19/Mcm21 CENP-P/O , selectively bound Cse4 CENP-A nucleosomes through the Cse4 N-terminus and thereby establishes a direct link to the outer kinetochore MTW1 complex. The Sli15/Ipl1 INCENP/Aurora B core-CPC interacted with COMA through the Ctf19 C-terminus, and artificial tethering of Sli15 to Ame1/Okp1 rescued synthetic lethality upon Ctf19/Mcm21 deletion in a Sli15 centromere-targeting deficient mutant. This study reveals characteristics of the inner kinetochore architecture assembled at point centromeres and the relevance of its Sli15/Ipl1 interaction for CPC function." @default.
- W2896677307 created "2018-10-26" @default.
- W2896677307 creator A5004939008 @default.
- W2896677307 creator A5005262220 @default.
- W2896677307 creator A5005275669 @default.
- W2896677307 creator A5033619686 @default.
- W2896677307 creator A5040932764 @default.
- W2896677307 creator A5054539660 @default.
- W2896677307 creator A5065451251 @default.
- W2896677307 creator A5079706373 @default.
- W2896677307 creator A5085624953 @default.
- W2896677307 date "2018-10-16" @default.
- W2896677307 modified "2023-09-27" @default.
- W2896677307 title "The COMA complex is required for positioning Ipl1 activity proximal to Cse4 nucleosomes in budding yeast" @default.
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- W2896677307 doi "https://doi.org/10.1101/444570" @default.