FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896685648> ?p ?o ?g. }

• W2896685648 endingPage "222" @default.
• W2896685648 startingPage "212" @default.
• W2896685648 abstract "The daily shedding and renewal of photoreceptor outer segments (OS) is critical for maintaining vision. This process relies on the efficient uptake, degradation, and sorting of shed OS material by the retinal pigment epithelium (RPE). Poor OS degradation has been linked to retinal degenerations such as Stargardt disease and may contribute to macular degeneration. While primary human fetal RPE cultures have emerged as a valuable model of in vivo human RPE function, surprisingly few studies have utilized the model for tracking the degradation and fate of OS components in the RPE. Here, we establish an improved platform for studying this topic by modifying existing protocols and creating new methods. Our human fetal culture model facilitates studies of RPE secretion in response to OS ingestion, preserves RPE differentiation and polarization during live-cell imaging of OS phagocytosis, and minimizes costs. We optimize Mer tyrosine kinase-dependent OS phagocytosis assays specifically in human fetal cultures and provide a simple and accurate method for measuring total OS consumption by the RPE. Finally, we utilize chemical transfection, dextran labeling, and immunocytochemistry to evaluate key players in OS degradation, including lysosomes and autophagy proteins. To facilitate quantification of autophagy vesicles, we develop customized image analysis macros in the Fiji/ImageJ software environment. These protocols will facilitate a broad range of studies in human fetal RPE cultures aimed at determining the ultimate fate of OS components after ingestion, a critical step in understanding the pathogenesis of numerous retinal diseases." @default.
• W2896685648 created "2018-10-26" @default.
• W2896685648 creator A5001456988 @default.
• W2896685648 creator A5002605015 @default.
• W2896685648 creator A5018830876 @default.
• W2896685648 creator A5034047007 @default.
• W2896685648 creator A5045228502 @default.
• W2896685648 creator A5068393306 @default.
• W2896685648 creator A5072394648 @default.
• W2896685648 creator A5081804027 @default.
• W2896685648 date "2019-01-01" @default.
• W2896685648 modified "2023-10-16" @default.
• W2896685648 title "A platform for assessing outer segment fate in primary human fetal RPE cultures" @default.
• W2896685648 cites W1026154262 @default.
• W2896685648 cites W1593864683 @default.
• W2896685648 cites W1971244021 @default.
• W2896685648 cites W1973207504 @default.
• W2896685648 cites W1976263073 @default.
• W2896685648 cites W1978462231 @default.
• W2896685648 cites W1978810668 @default.
• W2896685648 cites W1979193466 @default.
• W2896685648 cites W1979411643 @default.
• W2896685648 cites W1988155168 @default.
• W2896685648 cites W1992416493 @default.
• W2896685648 cites W1993913137 @default.
• W2896685648 cites W1995732106 @default.
• W2896685648 cites W2015363453 @default.
• W2896685648 cites W2021788996 @default.
• W2896685648 cites W2032730097 @default.
• W2896685648 cites W2039949937 @default.
• W2896685648 cites W2044829692 @default.
• W2896685648 cites W2046174024 @default.
• W2896685648 cites W2052031900 @default.
• W2896685648 cites W2053670812 @default.
• W2896685648 cites W2082211576 @default.
• W2896685648 cites W2082637970 @default.
• W2896685648 cites W2087386497 @default.
• W2896685648 cites W2125278527 @default.
• W2896685648 cites W2127327360 @default.
• W2896685648 cites W2130548721 @default.
• W2896685648 cites W2147481857 @default.
• W2896685648 cites W2148786460 @default.
• W2896685648 cites W2150474659 @default.
• W2896685648 cites W2152331121 @default.
• W2896685648 cites W2167279371 @default.
• W2896685648 cites W2299041215 @default.
• W2896685648 cites W2319608261 @default.
• W2896685648 cites W2323603734 @default.
• W2896685648 cites W2330533782 @default.
• W2896685648 cites W2340180727 @default.
• W2896685648 cites W2469879265 @default.
• W2896685648 cites W2531412645 @default.
• W2896685648 cites W2750719761 @default.
• W2896685648 cites W2764102165 @default.
• W2896685648 cites W2951485368 @default.
• W2896685648 cites W54117844 @default.
• W2896685648 doi "https://doi.org/10.1016/j.exer.2018.10.008" @default.
• W2896685648 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6426633" @default.
• W2896685648 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30336126" @default.
• W2896685648 hasPublicationYear "2019" @default.
• W2896685648 type Work @default.
• W2896685648 sameAs 2896685648 @default.
• W2896685648 citedByCount "7" @default.
• W2896685648 countsByYear W28966856482019 @default.
• W2896685648 countsByYear W28966856482020 @default.
• W2896685648 countsByYear W28966856482021 @default.
• W2896685648 countsByYear W28966856482023 @default.
• W2896685648 crossrefType "journal-article" @default.
• W2896685648 hasAuthorship W2896685648A5001456988 @default.
• W2896685648 hasAuthorship W2896685648A5002605015 @default.
• W2896685648 hasAuthorship W2896685648A5018830876 @default.
• W2896685648 hasAuthorship W2896685648A5034047007 @default.
• W2896685648 hasAuthorship W2896685648A5045228502 @default.
• W2896685648 hasAuthorship W2896685648A5068393306 @default.
• W2896685648 hasAuthorship W2896685648A5072394648 @default.
• W2896685648 hasAuthorship W2896685648A5081804027 @default.
• W2896685648 hasBestOaLocation W28966856482 @default.
• W2896685648 hasConcept C118487528 @default.
• W2896685648 hasConcept C190283241 @default.
• W2896685648 hasConcept C203522944 @default.
• W2896685648 hasConcept C2776403814 @default.
• W2896685648 hasConcept C2778024200 @default.
• W2896685648 hasConcept C2780827179 @default.
• W2896685648 hasConcept C55493867 @default.
• W2896685648 hasConcept C71924100 @default.
• W2896685648 hasConcept C86803240 @default.
• W2896685648 hasConcept C95444343 @default.
• W2896685648 hasConceptScore W2896685648C118487528 @default.
• W2896685648 hasConceptScore W2896685648C190283241 @default.
• W2896685648 hasConceptScore W2896685648C203522944 @default.
• W2896685648 hasConceptScore W2896685648C2776403814 @default.
• W2896685648 hasConceptScore W2896685648C2778024200 @default.
• W2896685648 hasConceptScore W2896685648C2780827179 @default.
• W2896685648 hasConceptScore W2896685648C55493867 @default.
• W2896685648 hasConceptScore W2896685648C71924100 @default.
• W2896685648 hasConceptScore W2896685648C86803240 @default.
• W2896685648 hasConceptScore W2896685648C95444343 @default.
• W2896685648 hasFunder F4320332161 @default.

• next