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- W2896733464 abstract "Copyright information: Taken from Characterization of RNase R-digested cellular RNA source that consists of lariat and circular RNAs from pre-mRNA splicingNucleic Acids Research 2006;34(8):e63-e63.Published online 8 May 2006PMCID:PMC1458517.© The Author 2006. Published by Oxford University Press. All rights reserved Human β-globin pre-mRNA was spliced with HeLa cell nuclear extract (lane 1) and the RNA products were further digested with each RNase for indicated incubation time (min; lanes 2–10). The RNAs were analyzed by denaturing 5.5% PAGE. The positions of splicing products and RNase-digested products are schematically indicated with their structures. DNA markers (New England Biolabs) are shown with their sizes (M). () Analyses of the lariat RNAs by debranching. spliced products and their RNase-digested RNA products [lanes 11–14; reactions are corresponding to lanes 1, 3, 7 and 9 in (A), respectively] were debranched with HeLa cell S100 extract (lanes 15–18). The RNAs were analyzed by denaturing 9.0% PAGE. The positions of RNA products are schematically represented with their structures. () Digestion pathway of the 3′ tail of lariat RNA by RNases and debranching of the products." @default.
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- W2896733464 date "2011-01-01" @default.
- W2896733464 modified "2023-09-23" @default.
- W2896733464 title "() Digestions of the splicing products with RNase R, RNase II and PNPase" @default.
- W2896733464 doi "https://doi.org/10.6084/m9.figshare.39711" @default.
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