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- W2896733598 abstract "In this chapter, we introduce a nanodisc-based experimental platform to study Ca2+-triggered membrane interaction of synaptotagmin-1. We describe and discuss in detail how to assemble this soluble mimetic of the docked vesicle–plasma membrane junction, with fluorescently labeled synaptotagmin-1 bound to trans SNAREpins assembled between nanodiscs and present the stopped-flow rapid mixing method used to monitor the conformational dynamics of Ca2+-activation process on a millisecond timescale." @default.
- W2896733598 created "2018-10-26" @default.
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- W2896733598 date "2018-10-14" @default.
- W2896733598 modified "2023-10-16" @default.
- W2896733598 title "Using Nanodiscs to Probe Ca2+-Dependent Membrane Interaction of Synaptotagmin-1" @default.
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- W2896733598 doi "https://doi.org/10.1007/978-1-4939-8760-3_14" @default.
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