FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896763304> ?p ?o ?g. }

• W2896763304 endingPage "11395" @default.
• W2896763304 startingPage "11384" @default.
• W2896763304 abstract "Insufficient supply of Met and choline (Chol) around parturition could compromise hepatic metabolism and milk protein synthesis in dairy cows. Mechanistic responses associated with supply of Met or Chol in primary liver cells enriched with hepatocytes (PHEP) from cows have not been thoroughly ascertained. Objectives were to isolate and culture PHEP to examine abundance of genes and proteins related to transmethylation, transsulfuration, and cytidine 5'-diphosphocholine (CDP-choline) pathways in response to Met or Chol. The PHEP were isolated from liver biopsies of Holstein cows (160 d in lactation). More than 90% of isolated cells stained positively for the hepatocyte marker cytokeratin 18. Cytochrome P450 (CYP1A1) mRNA abundance was only detectable in the PHEP and liver tissue compared with mammary tissue. Furthermore, in response to exogenous Met (80 μM vs. control) PHEP secreted greater amounts of albumin and urea. Subsequently, PHEP were cultured with Met (40 μM) or Chol (80 mg/dL) for 24 h. Compared with control or Chol, mRNA and protein abundance of methionine adenosyltransferase 1A (MAT1A) and phosphatidylethanolamine methyltransferase (PEMT) were greater in PHEP treated with Met. The mRNA abundance of S-adenosylhomocysteine hydrolase (SAHH), betaine-homocysteine methyltransferase (BHMT), and sarcosine dehydrogenase (SARDH) was greater in Met-treated PHEP compared with control or Chol. Compared with control, greater expression of 5-methyltetrahydrofolate-homocysteine methyltransferase (MTR), betaine aldehyde dehydrogenase (BADH), and choline dehydrogenase (CHDH) was observed in cells supplemented with Met and Chol. However, Chol led to the greatest mRNA abundance of CHDH. Abundance of choline kinase α (CHKA), choline kinase β (CHKB), phosphate cytidylyltransferase 1 α (PCYT1A), and choline/ethanolamine phosphotransferase 1 (CEPT1) in the CDP-choline pathway was greater in PHEP treated with Chol compared with control or Met. In the transsulfuration pathway, mRNA and protein abundance of cystathionine β-synthase (CBS) was greater in PHEP treated with Met compared with control or Chol. Similarly, abundance of cysteine sulfinic acid decarboxylase (CSAD), glutamate-cysteine ligase, catalytic subunit (GCLC), and glutathione reductase (GSR) was greater in response to Met compared with control or Chol. Overall, these findings suggest that transmethylation and transsulfuration in dairy cow primary liver cells are more responsive to Met supply, whereas the CDP-choline pathway is more responsive to Chol supply. The relevance of these data in vivo merit further study." @default.
• W2896763304 created "2018-10-26" @default.
• W2896763304 creator A5005157936 @default.
• W2896763304 creator A5025156989 @default.
• W2896763304 creator A5026336075 @default.
• W2896763304 creator A5030215514 @default.
• W2896763304 creator A5049777665 @default.
• W2896763304 creator A5078504589 @default.
• W2896763304 creator A5083096746 @default.
• W2896763304 date "2018-12-01" @default.
• W2896763304 modified "2023-10-16" @default.
• W2896763304 title "Methionine and choline supply alter transmethylation, transsulfuration, and cytidine 5′-diphosphocholine pathways to different extents in isolated primary liver cells from dairy cows" @default.
• W2896763304 cites W1929448476 @default.
• W2896763304 cites W1962867466 @default.
• W2896763304 cites W1964907817 @default.
• W2896763304 cites W1967214377 @default.
• W2896763304 cites W1972660602 @default.
• W2896763304 cites W1980265498 @default.
• W2896763304 cites W1981689223 @default.
• W2896763304 cites W1982589515 @default.
• W2896763304 cites W1987438135 @default.
• W2896763304 cites W2001779904 @default.
• W2896763304 cites W2002583208 @default.
• W2896763304 cites W2010268291 @default.
• W2896763304 cites W2015529097 @default.
• W2896763304 cites W2017025083 @default.
• W2896763304 cites W2020248727 @default.
• W2896763304 cites W2023190763 @default.
• W2896763304 cites W2024428225 @default.
• W2896763304 cites W2027005520 @default.
• W2896763304 cites W2027360280 @default.
• W2896763304 cites W2031154405 @default.
• W2896763304 cites W2035588804 @default.
• W2896763304 cites W2043037824 @default.
• W2896763304 cites W2048349001 @default.
• W2896763304 cites W2051256772 @default.
• W2896763304 cites W2057833729 @default.
• W2896763304 cites W2069301707 @default.
• W2896763304 cites W2073178584 @default.
• W2896763304 cites W2077025306 @default.
• W2896763304 cites W2077946349 @default.
• W2896763304 cites W2083156321 @default.
• W2896763304 cites W2084184770 @default.
• W2896763304 cites W2109865038 @default.
• W2896763304 cites W2146669705 @default.
• W2896763304 cites W2148559321 @default.
• W2896763304 cites W2151673941 @default.
• W2896763304 cites W2157175366 @default.
• W2896763304 cites W2157370926 @default.
• W2896763304 cites W2169761177 @default.
• W2896763304 cites W2178820846 @default.
• W2896763304 cites W2286199495 @default.
• W2896763304 cites W2298466016 @default.
• W2896763304 cites W2321153986 @default.
• W2896763304 cites W2492260544 @default.
• W2896763304 cites W2511656868 @default.
• W2896763304 cites W2519953976 @default.
• W2896763304 cites W2520894026 @default.
• W2896763304 cites W2527770420 @default.
• W2896763304 cites W2556330098 @default.
• W2896763304 cites W2583321150 @default.
• W2896763304 cites W2585787843 @default.
• W2896763304 cites W2734739376 @default.
• W2896763304 cites W2736204751 @default.
• W2896763304 cites W2766225824 @default.
• W2896763304 cites W2773288828 @default.
• W2896763304 cites W2790996470 @default.
• W2896763304 doi "https://doi.org/10.3168/jds.2017-14236" @default.
• W2896763304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30316602" @default.
• W2896763304 hasPublicationYear "2018" @default.
• W2896763304 type Work @default.
• W2896763304 sameAs 2896763304 @default.
• W2896763304 citedByCount "24" @default.
• W2896763304 countsByYear W28967633042019 @default.
• W2896763304 countsByYear W28967633042020 @default.
• W2896763304 countsByYear W28967633042021 @default.
• W2896763304 countsByYear W28967633042022 @default.
• W2896763304 countsByYear W28967633042023 @default.
• W2896763304 crossrefType "journal-article" @default.
• W2896763304 hasAuthorship W2896763304A5005157936 @default.
• W2896763304 hasAuthorship W2896763304A5025156989 @default.
• W2896763304 hasAuthorship W2896763304A5026336075 @default.
• W2896763304 hasAuthorship W2896763304A5030215514 @default.
• W2896763304 hasAuthorship W2896763304A5049777665 @default.
• W2896763304 hasAuthorship W2896763304A5078504589 @default.
• W2896763304 hasAuthorship W2896763304A5083096746 @default.
• W2896763304 hasBestOaLocation W28967633041 @default.
• W2896763304 hasConcept C104317684 @default.
• W2896763304 hasConcept C153911025 @default.
• W2896763304 hasConcept C16525657 @default.
• W2896763304 hasConcept C181199279 @default.
• W2896763304 hasConcept C185592680 @default.
• W2896763304 hasConcept C2777248517 @default.
• W2896763304 hasConcept C2777468819 @default.
• W2896763304 hasConcept C2778377652 @default.
• W2896763304 hasConcept C2780912031 @default.
• W2896763304 hasConcept C2781123555 @default.
• W2896763304 hasConcept C2781184954 @default.

• next