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• W2896764488 abstract "Ribosome profiling accesses the translational step of gene expression via deep sequencing of ribosome-protected mRNA footprints. Pairing of ribosome profiling and transcriptomics data provides a translational efficiency for each gene. Here, the translatome and transcriptome of the model cyanobacterium Synechocystis were compared under carbon-replete and carbon starvation conditions. The latter may be experienced when cyanobacteria are cultivated in poorly mixed bioreactors or engineered to be product-secreting cell factories. A small fraction of genes (<200), including stress response genes, showed changes in translational efficiency during carbon starvation, indicating condition-dependent translation-level regulation. We observed ribosome occupancy in untranslated regions, possibly due to an alternative translation initiation mechanism in Synechocystis. The higher proportion of ribosomes residing in untranslated regions during carbon starvation may be a mechanism to quickly inactivate superfluous ribosomes. This work provides the first ribosome profiling data for cyanobacteria and reveals new regulation strategies for coping with nutrient limitation." @default.
• W2896764488 created "2018-10-26" @default.
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• W2896764488 date "2018-10-30" @default.
• W2896764488 modified "2023-10-01" @default.
• W2896764488 title "Ribosome Profiling of <i>Synechocystis</i> Reveals Altered Ribosome Allocation at Carbon Starvation" @default.
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• W2896764488 doi "https://doi.org/10.1128/msystems.00126-18" @default.
• W2896764488 hasPublicationYear "2018" @default.
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