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• W2896764626 abstract "Abstract Isaria farinosa is a pathogen of alpine Thitarodes larvae that are hosts for the Chinese medicinal fungus, Ophiocordyceps sinensis . A matrix analysis indicated that the optimal culture conditions for the mycelial growth of I . farinosa are a 50-mL liquid broth in a 250-mL flask at more than 100-rpm rotation and 15–25 °C. Illumination does not affect the mycelial growth. The optimal nutrition requirements are D-(+)-galactose and D-(−)-fructose as carbon resources and D-cysteine as well as yeast powder, peptone, and beef extract as nitrogen resources at a carbon-to-nitrogen ratio of 1:1 to 1:7. The mineral component and vitamins also significantly increase the mycelial growth of I . farinosa . Based on the optimal culture conditions and nutrition requirements for the mycelial growth of I . farinosa , the effects of altitude on mycelial growth and its metabolome were evaluated using quadrupole-time-of-flight/mass spectrometry, principal component analysis and partial least squares discriminant analysis. The altitude did not affect the mycelial production but significantly regulated its metabolome. The study presents a new approach to better select a method for producing more useful metabolites and highlights the necessity of establishing standards for culturing methods related to altitude to preserve fungal quality; additionally, the results indicate that the use of a fermenter may meet the demands of large-scale mycelial production." @default.
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• W2896764626 date "2018-10-23" @default.
• W2896764626 modified "2023-10-13" @default.
• W2896764626 title "Culture conditions and nutrition requirements for the mycelial growth of Isaria farinosa (Hypocreales: Cordycipitaceae) and the altitude effect on its growth and metabolome" @default.
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• W2896764626 doi "https://doi.org/10.1038/s41598-018-33965-z" @default.
• W2896764626 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6199337" @default.
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