Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896765819> ?p ?o ?g. }
- W2896765819 endingPage "589" @default.
- W2896765819 startingPage "576" @default.
- W2896765819 abstract "Low back pain is a major cause for disability and is closely linked to intervertebral disc degeneration. Mechanical and biological dysfunction of the nucleus pulposus in the disc has been found to initiate intradiscal degenerative processes. Replacing or enriching the diseased nucleus pulposus with an injectable, stem cell-laden biomaterial that mimics its material properties can provide a minimally invasive strategy for biological and structural repair of the tissue. In this study, injectable, in situ-gelling carboxymethylcellulose hydrogels were developed for nucleus pulposus tissue engineering using encapsulated human marrow-derived mesenchymal stromal cells (hMSCs). With the goal of obtaining robust extracellular matrix deposition and faster construct maturation, two cell-seeding densities, 20 × 10 6 cells/ml and 40 × 10 6 cells/ml, were examined. The constructs were fabricated using a redox initiation system to yield covalently crosslinked, cell-seeded hydrogels via radical polymerization. Chondrogenic culture of the hydrogels over 35 days exhibited high cell viability along with deposition of proteoglycan and collagen-rich extracellular matrix, and mechanical and swelling properties similar to native human nucleus pulposus. Further, the matrix production and distribution in the carboxymethylcellulose hydrogels was found to be strongly influenced by hMSC-seeding density, with the lower cell-seeding density yielding a more favorable nucleus pulposus-specific matrix phenotype, while the rate of construct maturation was less dependent on the cell-seeding density. These findings are the first to demonstrate the utility of redox-polymerized carboxymethylcellulose hydrogels as hMSC carriers for potential minimally invasive treatment strategies for nucleus pulposus replacement." @default.
- W2896765819 created "2018-10-26" @default.
- W2896765819 creator A5001436452 @default.
- W2896765819 creator A5069012033 @default.
- W2896765819 creator A5074323203 @default.
- W2896765819 date "2018-10-01" @default.
- W2896765819 modified "2023-10-17" @default.
- W2896765819 title "Injectable, redox-polymerized carboxymethylcellulose hydrogels promote nucleus pulposus-like extracellular matrix elaboration by human MSCs in a cell density-dependent manner" @default.
- W2896765819 cites W141721208 @default.
- W2896765819 cites W1485385770 @default.
- W2896765819 cites W1531471675 @default.
- W2896765819 cites W1672305626 @default.
- W2896765819 cites W1817479092 @default.
- W2896765819 cites W1964531538 @default.
- W2896765819 cites W1968516916 @default.
- W2896765819 cites W1970165524 @default.
- W2896765819 cites W1972066775 @default.
- W2896765819 cites W1973996314 @default.
- W2896765819 cites W1978210260 @default.
- W2896765819 cites W1981616328 @default.
- W2896765819 cites W1982457774 @default.
- W2896765819 cites W1984726111 @default.
- W2896765819 cites W1985286917 @default.
- W2896765819 cites W1985880055 @default.
- W2896765819 cites W1987872284 @default.
- W2896765819 cites W1989199493 @default.
- W2896765819 cites W1991986285 @default.
- W2896765819 cites W1995071146 @default.
- W2896765819 cites W2003867975 @default.
- W2896765819 cites W2007413067 @default.
- W2896765819 cites W2007505738 @default.
- W2896765819 cites W2008488096 @default.
- W2896765819 cites W2008882114 @default.
- W2896765819 cites W2010484901 @default.
- W2896765819 cites W2010775152 @default.
- W2896765819 cites W2016117092 @default.
- W2896765819 cites W2018458290 @default.
- W2896765819 cites W2020342708 @default.
- W2896765819 cites W2022106720 @default.
- W2896765819 cites W2022119002 @default.
- W2896765819 cites W2026220322 @default.
- W2896765819 cites W2027990128 @default.
- W2896765819 cites W2028368206 @default.
- W2896765819 cites W2030604088 @default.
- W2896765819 cites W2031545788 @default.
- W2896765819 cites W2032393093 @default.
- W2896765819 cites W2036501293 @default.
- W2896765819 cites W2038857063 @default.
- W2896765819 cites W2043234704 @default.
- W2896765819 cites W2050475565 @default.
- W2896765819 cites W2051733665 @default.
- W2896765819 cites W2056120442 @default.
- W2896765819 cites W2057094501 @default.
- W2896765819 cites W2057635936 @default.
- W2896765819 cites W2057948940 @default.
- W2896765819 cites W2061768827 @default.
- W2896765819 cites W2062217024 @default.
- W2896765819 cites W2064003107 @default.
- W2896765819 cites W2065933206 @default.
- W2896765819 cites W2071187606 @default.
- W2896765819 cites W2072205513 @default.
- W2896765819 cites W2075116788 @default.
- W2896765819 cites W2076854724 @default.
- W2896765819 cites W2077309660 @default.
- W2896765819 cites W2077930888 @default.
- W2896765819 cites W2077940158 @default.
- W2896765819 cites W2080398161 @default.
- W2896765819 cites W2090346500 @default.
- W2896765819 cites W2090732472 @default.
- W2896765819 cites W2092398224 @default.
- W2896765819 cites W2094429476 @default.
- W2896765819 cites W2096639686 @default.
- W2896765819 cites W2100994995 @default.
- W2896765819 cites W2124694620 @default.
- W2896765819 cites W2126424577 @default.
- W2896765819 cites W2133526415 @default.
- W2896765819 cites W2135492990 @default.
- W2896765819 cites W2144815200 @default.
- W2896765819 cites W2151707307 @default.
- W2896765819 cites W2152983876 @default.
- W2896765819 cites W2159641941 @default.
- W2896765819 cites W2163998809 @default.
- W2896765819 cites W2166697224 @default.
- W2896765819 cites W2166996972 @default.
- W2896765819 cites W2169018506 @default.
- W2896765819 cites W2169444767 @default.
- W2896765819 cites W2176300883 @default.
- W2896765819 cites W2187995296 @default.
- W2896765819 cites W2410249905 @default.
- W2896765819 cites W2515556435 @default.
- W2896765819 cites W2521591018 @default.
- W2896765819 cites W2586673911 @default.
- W2896765819 cites W2596972615 @default.
- W2896765819 cites W2737467796 @default.
- W2896765819 cites W2739294464 @default.
- W2896765819 cites W2767086944 @default.
- W2896765819 cites W4230932862 @default.
- W2896765819 cites W42830026 @default.