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• W2896767833 abstract "Proteins are widely used in food or pharmaceutical industry and are often pumped in complex flow systems. Their biological activity is intrinsically related to their conformation in solution. In this work, we studied the state and behavior of proteins in solution during separation/concentration operation in terms of adsorption, molecule agglomeration, and denaturation. Solutions of lysozyme have been filtrated on a ceramic ultrafiltration membrane at high flow velocity and different transmembrane pressures to investigate the effect of flow parameters on the protein conformation in solution assessed by size-exclusion chromatography. A shift of 0.4 min in elution time of the peak corresponding to lysozyme is observed between retentate and permeate solutions, indicating that the lysozyme molecules exhibit drastic conformation changes after filtration. In addition, the effect of the filtrated solute nature on the pore surface (adsorption phenomena) is investigated by following the evolution of the hydraulic and selectivity membrane performances. Les solutions contenant des protéines sont souvent utilisés dans les industries pharmaceutiques et agroalimentaires, et sont souvent manipulées dans des systèmes fluides complexes. Leur activité biologique est directement reliée à la conformation des molécules en solution. Dans cette étude, l'état et le comportement de protéines en solution ont été étudiés lors d'opérations de séparation à travers les propriétés d'adsorption, d'agglomération et de dénaturation des protéines. Des solutions de lysozyme ont été filtrées avec une membrane céramique d'ultrafiltration à bas seuil de coupure pour étudier l'effet des paramètres opératoires (vitesse, pression) sur la conformation de la protéine. Les résultats obtenus par chromatographie liquide d'exclusion stérique montre un décalage de 0.4 min du pic correspondant à la lysozyme après filtration, indiquant une modification importante de sa conformation. En parallèle, les phénomènes d'adsorption sont étudiés en suivant les propriétés hydrauliques et de sélectivité de la membrane." @default.
• W2896767833 created "2018-10-26" @default.
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• W2896767833 date "2019-02-01" @default.
• W2896767833 modified "2023-10-08" @default.
• W2896767833 title "Filtration of protein-based solutions with ceramic ultrafiltration membrane. Study of selectivity, adsorption, and protein denaturation" @default.
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• W2896767833 doi "https://doi.org/10.1016/j.crci.2018.09.011" @default.
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