Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896768595> ?p ?o ?g. }
- W2896768595 abstract "Fe-S clusters are ubiquitous cofactors of proteins involved in a variety of essential cellular processes. The biogenesis of Fe-S clusters in the cytosol and their insertion into proteins is accomplished through the cytosolic iron-sulphur protein assembly (CIA) machinery. The early- and middle-acting modules of the CIA pathway concerned with the assembly and trafficking of Fe-S clusters have been previously characterised in the parasitic protist Trypanosoma brucei. In this study, we applied proteomic and genetic approaches to gain insights into the network of protein-protein interactions of the late-acting CIA targeting complex in T. brucei. All components of the canonical CIA machinery are present in T. brucei including, as in humans, two distinct CIA2 homologues TbCIA2A and TbCIA2B. These two proteins are found interacting with TbCIA1, yet the interaction is mutually exclusive, as determined by mass spectrometry. Ablation of most of the components of the CIA targeting complex by RNAi led to impaired cell growth in vitro, with the exception of TbCIA2A in procyclic form (PCF) trypanosomes. Depletion of the CIA-targeting complex was accompanied by reduced levels of protein-bound cytosolic iron and decreased activity of an Fe-S dependent enzyme in PCF trypanosomes. We demonstrate that the C-terminal domain of TbMMS19 acts as a docking site for TbCIA2B and TbCIA1, forming a trimeric complex that also interacts with target Fe-S apo-proteins and the middle-acting CIA component TbNAR1." @default.
- W2896768595 created "2018-10-26" @default.
- W2896768595 creator A5011681651 @default.
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- W2896768595 date "2018-10-22" @default.
- W2896768595 modified "2023-10-12" @default.
- W2896768595 title "Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei" @default.
- W2896768595 cites W1491168530 @default.
- W2896768595 cites W1494892052 @default.
- W2896768595 cites W1497947649 @default.
- W2896768595 cites W1517190078 @default.
- W2896768595 cites W1564503944 @default.
- W2896768595 cites W1581072312 @default.
- W2896768595 cites W170154559 @default.
- W2896768595 cites W1803102843 @default.
- W2896768595 cites W1901786564 @default.
- W2896768595 cites W190723275 @default.
- W2896768595 cites W1918067589 @default.
- W2896768595 cites W1923688046 @default.
- W2896768595 cites W1938415412 @default.
- W2896768595 cites W1967320212 @default.
- W2896768595 cites W1967668810 @default.
- W2896768595 cites W1969743438 @default.
- W2896768595 cites W1976051657 @default.
- W2896768595 cites W1976725682 @default.
- W2896768595 cites W1984679061 @default.
- W2896768595 cites W1988678216 @default.
- W2896768595 cites W1988730883 @default.
- W2896768595 cites W1993743888 @default.
- W2896768595 cites W1994844474 @default.
- W2896768595 cites W2002783267 @default.
- W2896768595 cites W2003317994 @default.
- W2896768595 cites W2009029742 @default.
- W2896768595 cites W2018506581 @default.
- W2896768595 cites W2025028167 @default.
- W2896768595 cites W2029942494 @default.
- W2896768595 cites W2032778333 @default.
- W2896768595 cites W2033063558 @default.
- W2896768595 cites W2033163051 @default.
- W2896768595 cites W2035199905 @default.
- W2896768595 cites W2044085946 @default.
- W2896768595 cites W2044101358 @default.
- W2896768595 cites W2049521543 @default.
- W2896768595 cites W2050828231 @default.
- W2896768595 cites W2052916872 @default.
- W2896768595 cites W2056464081 @default.
- W2896768595 cites W2058145203 @default.
- W2896768595 cites W2060259273 @default.
- W2896768595 cites W2061642409 @default.
- W2896768595 cites W2061934709 @default.
- W2896768595 cites W2066810740 @default.
- W2896768595 cites W2069606939 @default.
- W2896768595 cites W2073011296 @default.
- W2896768595 cites W2073302338 @default.
- W2896768595 cites W2073746011 @default.
- W2896768595 cites W2074844635 @default.
- W2896768595 cites W2078073818 @default.
- W2896768595 cites W2088834030 @default.
- W2896768595 cites W2092018396 @default.
- W2896768595 cites W2093682855 @default.
- W2896768595 cites W2095217602 @default.
- W2896768595 cites W2096985831 @default.
- W2896768595 cites W2097666362 @default.
- W2896768595 cites W2099540110 @default.
- W2896768595 cites W2103387251 @default.
- W2896768595 cites W2105121902 @default.
- W2896768595 cites W2107915297 @default.
- W2896768595 cites W2108465871 @default.
- W2896768595 cites W2109009897 @default.
- W2896768595 cites W2111565312 @default.
- W2896768595 cites W2112632190 @default.
- W2896768595 cites W2112743424 @default.
- W2896768595 cites W2114220644 @default.
- W2896768595 cites W2116350092 @default.
- W2896768595 cites W2120269671 @default.
- W2896768595 cites W2123139638 @default.
- W2896768595 cites W2125112712 @default.
- W2896768595 cites W2140481794 @default.
- W2896768595 cites W2141690385 @default.
- W2896768595 cites W2142528445 @default.
- W2896768595 cites W2143361020 @default.
- W2896768595 cites W2147567178 @default.
- W2896768595 cites W2150608129 @default.
- W2896768595 cites W2152158810 @default.
- W2896768595 cites W2157236463 @default.
- W2896768595 cites W2159700780 @default.
- W2896768595 cites W2160293546 @default.
- W2896768595 cites W2161456251 @default.
- W2896768595 cites W2167279371 @default.
- W2896768595 cites W2172659855 @default.
- W2896768595 cites W2173049382 @default.
- W2896768595 cites W2183183854 @default.