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• W2896771601 abstract "Stress leads to production of reactive oxygen species (ROS) and reactive nitrogen species (RNS). Among the RNS, nitric oxide (NO) is an important signaling molecule that mediates its effect majorly via a reversible post-translational modification (PTM) S-nitrosylation. However, information on the NO mediated cold stress signaling is still missing for nucleus. Therefore, in the present study an attempt was made to analyze NO signaling, particularly, S-nitrosylation in the nuclei of Brassica juncea seedlings. Cold stress (4 °C) induced nitrate reductase (NR) mediated NO production along with the accumulation of available protein thiols (5 fold, at 96 h, 4 °C) and low molecular weight thiols (1.2 fold at 12 h, 4 °C). S-nitrosoglutathione (GSNO, a NO donor) and cold (endogenous, S-nitrosylation without the addition of NO donor) treated, neutravidin-agarose affinity chromatography purified S-nitrosylated proteins were resolved as 55- and 32-spots respectively on the 2-D gels. Functional categorization after MALDI-TOF/TOF identification showed 67% targets to be stress/defense/redox related and 33% to be metabolic, suggesting the potential role(s) of S-nitrosylation in these responses. Cold stress increased S-nitrosylation of Cu/Zn SOD, l-ascorbate oxidase, fructose bisphosphate aldolase (FBA2) and malate dehydrogenase 1 (MDH1), therefore, indicating NO mediated regulation of redox and metabolism related pathways. Identification of targets of glucosinolate hydrolysis pathway (myrosinase and myrosinase-binding protein 2-like) indicated the role of S-nitrosylation in regulating mustard oil bomb. Presence of myrosinase was confirmed by activity measurements and western blotting. Increased S-nitrosylation of myrosinase in cold stress in the nucleus is reported for the first time. Here, we proposed S-nitrosylation mediated nuclear trafficking of non-nuclear targets such as beta-d-xylosidase, daikon cysteine protease, and beta-glucosidase to the nucleus. Similarly, identification of FBA2 and MDH1 in the nucleus, indicated the role of NO in modulating their moonlighting function via S-nitrosylation in stress. Present study provided the first repertoire of cold modulated S-nitrosylated proteins in the nucleus." @default.
• W2896771601 created "2018-10-26" @default.
• W2896771601 creator A5006198401 @default.
• W2896771601 creator A5036758048 @default.
• W2896771601 creator A5067517100 @default.
• W2896771601 date "2019-05-01" @default.
• W2896771601 modified "2023-09-24" @default.
• W2896771601 title "Cold modulated nuclear S-nitrosoproteome analysis indicates redox modulation of novel Brassicaceae specific, myrosinase and napin in Brassica juncea" @default.
• W2896771601 cites W1037789279 @default.
• W2896771601 cites W106527565 @default.
• W2896771601 cites W1581384829 @default.
• W2896771601 cites W1851546610 @default.
• W2896771601 cites W1930528640 @default.
• W2896771601 cites W1958228372 @default.
• W2896771601 cites W1967197771 @default.
• W2896771601 cites W1969947808 @default.
• W2896771601 cites W1973505370 @default.
• W2896771601 cites W1973864912 @default.
• W2896771601 cites W1975151138 @default.
• W2896771601 cites W1977535645 @default.
• W2896771601 cites W1979169122 @default.
• W2896771601 cites W1979697920 @default.
• W2896771601 cites W1980869069 @default.
• W2896771601 cites W1986123968 @default.
• W2896771601 cites W1986908600 @default.
• W2896771601 cites W1987645050 @default.
• W2896771601 cites W1992280189 @default.
• W2896771601 cites W1992497040 @default.
• W2896771601 cites W1993257224 @default.
• W2896771601 cites W1996843450 @default.
• W2896771601 cites W1999974239 @default.
• W2896771601 cites W2004292205 @default.
• W2896771601 cites W2004781188 @default.
• W2896771601 cites W2006491538 @default.
• W2896771601 cites W2009991051 @default.
• W2896771601 cites W2012352784 @default.
• W2896771601 cites W2016463863 @default.
• W2896771601 cites W2028806051 @default.
• W2896771601 cites W2028895959 @default.
• W2896771601 cites W2030864821 @default.
• W2896771601 cites W2038233249 @default.
• W2896771601 cites W2039902448 @default.
• W2896771601 cites W2044050143 @default.
• W2896771601 cites W2044075506 @default.
• W2896771601 cites W2044703052 @default.
• W2896771601 cites W2046927023 @default.
• W2896771601 cites W2047583219 @default.
• W2896771601 cites W2049752266 @default.
• W2896771601 cites W2054307806 @default.
• W2896771601 cites W2056623748 @default.
• W2896771601 cites W2057927753 @default.
• W2896771601 cites W2059357385 @default.
• W2896771601 cites W2060528512 @default.
• W2896771601 cites W2061419667 @default.
• W2896771601 cites W2065773243 @default.
• W2896771601 cites W2068159542 @default.
• W2896771601 cites W2072371666 @default.
• W2896771601 cites W2073198696 @default.
• W2896771601 cites W2076649422 @default.
• W2896771601 cites W2080296544 @default.
• W2896771601 cites W2086518087 @default.
• W2896771601 cites W2086758583 @default.
• W2896771601 cites W2088867481 @default.
• W2896771601 cites W2089534180 @default.
• W2896771601 cites W2089635402 @default.
• W2896771601 cites W2093710019 @default.
• W2896771601 cites W2101108802 @default.
• W2896771601 cites W2102918496 @default.
• W2896771601 cites W2103191284 @default.
• W2896771601 cites W2108123792 @default.
• W2896771601 cites W2108569306 @default.
• W2896771601 cites W2109533799 @default.
• W2896771601 cites W2110181121 @default.
• W2896771601 cites W2111127373 @default.
• W2896771601 cites W2113247978 @default.
• W2896771601 cites W2116380601 @default.
• W2896771601 cites W2117232429 @default.
• W2896771601 cites W2135500851 @default.
• W2896771601 cites W2139274441 @default.
• W2896771601 cites W2140807512 @default.
• W2896771601 cites W2143275830 @default.
• W2896771601 cites W2144059282 @default.
• W2896771601 cites W2146488556 @default.
• W2896771601 cites W2149912264 @default.
• W2896771601 cites W2150422717 @default.
• W2896771601 cites W2150563446 @default.
• W2896771601 cites W2150717161 @default.
• W2896771601 cites W2152611098 @default.
• W2896771601 cites W2155363641 @default.
• W2896771601 cites W2156549573 @default.
• W2896771601 cites W2157655779 @default.
• W2896771601 cites W2160492411 @default.
• W2896771601 cites W2163748001 @default.
• W2896771601 cites W2164516951 @default.
• W2896771601 cites W2164828140 @default.
• W2896771601 cites W2166632201 @default.
• W2896771601 cites W2166761952 @default.
• W2896771601 cites W2167311872 @default.

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