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• W2896772928 abstract "Visual DNA amplification using a simple polymerase chain reaction (PCR) device is useful for field tests to detect target DNA and RNA. We hereby describe a detection system involving PCR amplification visualized with the naked eye, by genetic alphabet expansion. The system employs fluorescence resonance energy transfer (FRET) between unnatural base combinations: self-quenched dinucleotides of 2-amino-6-(2-thienyl)purine (s) as a donor and Cy3-conjugated 2-nitro-4-propynylpyrrole (Cy3-hx-Px) as an acceptor. During PCR, the triphosphate substrate of Cy3-hx-Px (Cy3-hx-dPxTP) is incorporated into DNA opposite its pairing partner, 7-(2-thienyl)-imidazo[4,5- b]pyridine (Ds), in the primer, which also contains the dinucleotides of s. Thus, the amplified DNA can be visualized by the Cy3 fluorescence resulting from the FRET between the s-dinucleotides and the incorporated Cy3-hx-Px upon 365 nm irradiation. Using this system, we demonstrated the visual single nucleotide polymorphism detection of a series of quinolone-resistant bacteria genes." @default.
• W2896772928 created "2018-10-26" @default.
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• W2896772928 date "2018-10-15" @default.
• W2896772928 modified "2023-10-18" @default.
• W2896772928 title "Visual Detection of Amplified DNA by Polymerase Chain Reaction Using a Genetic Alphabet Expansion System" @default.
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• W2896772928 doi "https://doi.org/10.1021/jacs.8b08121" @default.
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• W2896772928 hasPublicationYear "2018" @default.
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