Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896775772> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2896775772 abstract "Objective Hypoxia or exercise could lead to oxidative stress. Hypoxia inducible factor-1 (HIF-1) is an oxygen sensor and the expression of its α subunit can be regulated by hypoxia. NF-E2-related factor 2 (Nrf2) is an important modifier of cellular responses to oxidative stress. A major mechanism in defense oxidative stress is the activation of the Nrf2-ARE antioxidant pathway. But whether the increase of HIF-1α could affect the Nrf2-ARE antioxidant signal, and further influence the oxidative stress status in vivo remains unknown. In this study, we wished to examine the effect of HIF-1α on Nrf2-ARE antioxidant pathway in mice skeletal muscle after acute exhaustion exercise.
 Methods HIF-1α high expression (H) and C57BL/6J mice(W) were used at 20 respectively and each kind of mice were randomly divided into two groups: control (C) and exercise (E). The treadmill exercise was preformed at the acute exhaustion exercise. On the day of acute exercise, mice allocated to perform treadmill running were subject to 5% incline and 5min at 10m/min, and then increased 3m/min every 3 minutes. Mice were sacrificed at the indicated time points following treadmill running.Nrf2, phosphor-Nrf2 (Ser40), nuclear Nrf2 protein were measured by Western Blot and Nrf2-ARE binding activity, the mRNA and proetin levels of Nrf2 target genes, key antioxidant enzymes (SOD1, SOD2, CAT, NQO-1) and ROS level, were also measured in skeletal muscles after the interventions.
 Results (1)The results showed that compared with WC, RNA and protein expression level of Nrf2 were increased in HC skeletal muscles. Nrf2-ARE binding activity, Nrf2 target gene SOD1, SOD2, NQO-1 mRNA expression and NQO-1 protein expression were also increased in HC skeletal muscles. Meanwhile, ROS level in HC skeletal muscles decreased significantly.
 (2) After the acute exhaustion exercise, high HIF-1α expression mice (HE) had higher expression of p-Nrf2(Ser 40) and nuclear Nrf2 protein than the wide type mice(WE). The mRNA expression of SOD1 and mRNA /protein of NQO-1 in HE increased as well. In contrast, ROS level decreased significantly in HE muscles.
 ConclusionsThe result indicated the proper high expression of HIF-1α could promote the antioxidant capacity of skeletal muscle in mice through Nrf2-ARE pathway." @default.
- W2896775772 created "2018-10-26" @default.
- W2896775772 creator A5011165078 @default.
- W2896775772 creator A5052228409 @default.
- W2896775772 creator A5068778108 @default.
- W2896775772 date "2018-10-04" @default.
- W2896775772 modified "2023-09-24" @default.
- W2896775772 title "PO-145 Effects of HIF-1α on Nrf2-ARE antioxidant signal in mice skeletal muscle after acute exhaustive exercise" @default.
- W2896775772 doi "https://doi.org/10.14428/ebr.v1i4.10483" @default.
- W2896775772 hasPublicationYear "2018" @default.
- W2896775772 type Work @default.
- W2896775772 sameAs 2896775772 @default.
- W2896775772 citedByCount "0" @default.
- W2896775772 crossrefType "journal-article" @default.
- W2896775772 hasAuthorship W2896775772A5011165078 @default.
- W2896775772 hasAuthorship W2896775772A5052228409 @default.
- W2896775772 hasAuthorship W2896775772A5068778108 @default.
- W2896775772 hasBestOaLocation W28967757721 @default.
- W2896775772 hasConcept C104317684 @default.
- W2896775772 hasConcept C126322002 @default.
- W2896775772 hasConcept C134018914 @default.
- W2896775772 hasConcept C178790620 @default.
- W2896775772 hasConcept C185592680 @default.
- W2896775772 hasConcept C2775838275 @default.
- W2896775772 hasConcept C2776108821 @default.
- W2896775772 hasConcept C2776151105 @default.
- W2896775772 hasConcept C2776415932 @default.
- W2896775772 hasConcept C2778004101 @default.
- W2896775772 hasConcept C2779959927 @default.
- W2896775772 hasConcept C2781464450 @default.
- W2896775772 hasConcept C48349386 @default.
- W2896775772 hasConcept C540031477 @default.
- W2896775772 hasConcept C55493867 @default.
- W2896775772 hasConcept C57600042 @default.
- W2896775772 hasConcept C71924100 @default.
- W2896775772 hasConcept C7836513 @default.
- W2896775772 hasConceptScore W2896775772C104317684 @default.
- W2896775772 hasConceptScore W2896775772C126322002 @default.
- W2896775772 hasConceptScore W2896775772C134018914 @default.
- W2896775772 hasConceptScore W2896775772C178790620 @default.
- W2896775772 hasConceptScore W2896775772C185592680 @default.
- W2896775772 hasConceptScore W2896775772C2775838275 @default.
- W2896775772 hasConceptScore W2896775772C2776108821 @default.
- W2896775772 hasConceptScore W2896775772C2776151105 @default.
- W2896775772 hasConceptScore W2896775772C2776415932 @default.
- W2896775772 hasConceptScore W2896775772C2778004101 @default.
- W2896775772 hasConceptScore W2896775772C2779959927 @default.
- W2896775772 hasConceptScore W2896775772C2781464450 @default.
- W2896775772 hasConceptScore W2896775772C48349386 @default.
- W2896775772 hasConceptScore W2896775772C540031477 @default.
- W2896775772 hasConceptScore W2896775772C55493867 @default.
- W2896775772 hasConceptScore W2896775772C57600042 @default.
- W2896775772 hasConceptScore W2896775772C71924100 @default.
- W2896775772 hasConceptScore W2896775772C7836513 @default.
- W2896775772 hasLocation W28967757721 @default.
- W2896775772 hasOpenAccess W2896775772 @default.
- W2896775772 hasPrimaryLocation W28967757721 @default.
- W2896775772 hasRelatedWork W1988436357 @default.
- W2896775772 hasRelatedWork W2001432494 @default.
- W2896775772 hasRelatedWork W2051559087 @default.
- W2896775772 hasRelatedWork W2313463101 @default.
- W2896775772 hasRelatedWork W2786192066 @default.
- W2896775772 hasRelatedWork W2791181644 @default.
- W2896775772 hasRelatedWork W2799908419 @default.
- W2896775772 hasRelatedWork W3204789122 @default.
- W2896775772 hasRelatedWork W48584652 @default.
- W2896775772 hasRelatedWork W611837283 @default.
- W2896775772 isParatext "false" @default.
- W2896775772 isRetracted "false" @default.
- W2896775772 magId "2896775772" @default.
- W2896775772 workType "article" @default.