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• W2896822247 abstract "In this report, recombinant human osteopontin synthesized in tobacco plants (p-rhOPN) is introduced as a potential bioactive molecule that can promote osteoblast adhesion and differentiation. A glass substrate (SiO2/Si-OH) grafted with poly(acrylic acid) (SiO2/Si-PAA) was prepared by surface-initiated reversible addition-fragmentation chain transfer polymerization and used as a carboxyl-rich platform for the chemical conjugation of p-rhOPN. The PAA grafting and subsequent p-rhOPN immobilization were confirmed by water contact angle, Fourier transform-infrared spectroscopy, X-ray photoelectron spectroscopy and atomic force microscopy analyses. Indirect ELISA quantification revealed that the p-rhOPN immobilization efficiency was above 95% and the surface coverage was a function of the p-rhOPN concentration. MC-3T3-E1 cells cultured on the SiO2/Si-PAA substrate immobilized with various concentrations (0.6–30 ng/mL) of p-rhOPN (SiO2/Si-p-rhOPN) exhibited superior cell spreading compared to those cultured on SiO2/Si-OH or gelatin-modified glass substrate (SiO2/Si-gelatin). Polymerase chain reaction analysis indicated that the SiO2/Si-p-rhOPN substrates with high level of immobilized p-rhOPN promoted MC-3T3-E1 cell differentiation, as demonstrated by the higher transcript expression levels of the osteogenic differentiation regulatory gene, Runt-related transcription factor 2, compared to cells cultured on SiO2/Si-OH or SiO2/Si-gelatin. Given that p-rhOPN can be more economically produced than the commercially available OPN derived from human or mammalian sources, then, together with its well-preserved biological function in spite of being chemically conjugated to the substrates, it is likely that p-rhOPN could be more broadly applied for the development of materials for bone tissue engineering with a promising medical and commercial value." @default.
• W2896822247 created "2018-10-26" @default.
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• W2896822247 date "2019-01-01" @default.
• W2896822247 modified "2023-10-17" @default.
• W2896822247 title "Surface-immobilized plant-derived osteopontin as an effective platform to promote osteoblast adhesion and differentiation" @default.
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• W2896822247 doi "https://doi.org/10.1016/j.colsurfb.2018.10.032" @default.
• W2896822247 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30551297" @default.
• W2896822247 hasPublicationYear "2019" @default.
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