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• W2896827342 endingPage "3025" @default.
• W2896827342 startingPage "3019" @default.
• W2896827342 abstract "Background: Regulatory T (Treg) cells are immunosuppressor lymphocytes that play a critical role in the establishmentand progression of cancers. A number of markers, especially FOXP3, CTLA-4 and GITR influence the function ofTreg cells. This investigation aimed to evaluate the expression of a number of important Treg cell-related markers byperipheral blood mononuclear cells (PBMCs) from newly-diagnosed women with breast cancer. Methods: The freshPBMCs were obtained from 20 women with breast cancer and 20 healthy individuals. The PBMCs from both groupswere cultured for 32 hours in the presence or absence of PHA (10 μg/ml). After total RNA extraction from culturedPBMCs, the expression of the FOXP3, CTLA-4 and GITR transcripts was assessed using real time-PCR. Results:The mRNA expression of FOXP3, CTLA-4 and GITR in unstimulated PBMCs from patients with breast cancer weresignificantly higher than healthy control group (P<0.05, PFOXP3, CTLA-4 and GITR transcripts in PHA-stimulated PBMCs from patients with breast cancer were significantlyincreased in comparison with healthy individuals (P<0.01, Pexpression of FOXP3, CTLA-4 and GITR represent higher activity of Treg cells in patients with breast cancer that mayplay an important role in the tumor establishment and development." @default.
• W2896827342 created "2018-10-26" @default.
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• W2896827342 date "2018-11-01" @default.
• W2896827342 modified "2023-10-18" @default.
• W2896827342 title "Overexpression of Regulatory T Cell-Related Markers (FOXP3, CTLA-4 and GITR) by Peripheral Blood Mononuclear Cells from Patients with Breast Cancer" @default.
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• W2896827342 doi "https://doi.org/10.31557/apjcp.2018.19.11.3019" @default.
• W2896827342 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6318404" @default.
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