Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896852537> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2896852537 abstract "Here, we developed a diagnostic ELISA for foot-and-mouth disease using recombinant occlusion bodies (rOBs) of baculovirus. We fused Δ3AB1–3, a polypeptide derived from non-structural proteins of foot-and-mouth disease virus, to polyhedrin (POLH), the major constituent of OBs, under polh promoter. To further assess the most convenient strategy to improve yields, we designed two recombinant baculoviruses, vPOLH and vPOLHE44G. These carried the sequence of the fusion protein POLH-Δ3AB1–3 with an additional copy in cis of polh or polhE44G, respectively, under p10 promoter. Our results show that both viruses expressed POLH-Δ3AB1–3, which was detected by western blot in purified rOBs with anti-POLH and anti-3AB1 antibodies. We also found that vPOLHE44G produced larger polyhedra and a significant increase of antigen yield (p < 0.01). Furthermore, the chimeric protein POLH-Δ3AB1–3 was recognized by sera from experimentally infected animals, showing that translational fusion to POLH does not alter the antigenicity of Δ3AB1–3. Finally, the rOBs were successfully used in an ELISA test to differentiate infected from vaccinated animals. Taken together, these results demonstrate the great potential of rOBs to develop diagnostic schemes adaptable to animal infectious diseases." @default.
- W2896852537 created "2018-10-26" @default.
- W2896852537 creator A5028368441 @default.
- W2896852537 creator A5028839300 @default.
- W2896852537 creator A5039992789 @default.
- W2896852537 creator A5066222130 @default.
- W2896852537 creator A5089605955 @default.
- W2896852537 date "2018-10-20" @default.
- W2896852537 modified "2023-10-10" @default.
- W2896852537 title "Recombinant occlusion bodies of baculovirus as carriers of a non-structural protein of foot-and-mouth disease virus" @default.
- W2896852537 cites W148280177 @default.
- W2896852537 cites W1979489081 @default.
- W2896852537 cites W2019116489 @default.
- W2896852537 cites W2021014107 @default.
- W2896852537 cites W2044911121 @default.
- W2896852537 cites W2051563241 @default.
- W2896852537 cites W2054361078 @default.
- W2896852537 cites W2092773540 @default.
- W2896852537 cites W2093551953 @default.
- W2896852537 cites W2095102293 @default.
- W2896852537 cites W2105753245 @default.
- W2896852537 cites W2137609419 @default.
- W2896852537 cites W2159424407 @default.
- W2896852537 cites W2570336575 @default.
- W2896852537 cites W2607025947 @default.
- W2896852537 cites W2768686967 @default.
- W2896852537 cites W2805260990 @default.
- W2896852537 doi "https://doi.org/10.1007/s13205-018-1482-x" @default.
- W2896852537 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6195870" @default.
- W2896852537 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30370198" @default.
- W2896852537 hasPublicationYear "2018" @default.
- W2896852537 type Work @default.
- W2896852537 sameAs 2896852537 @default.
- W2896852537 citedByCount "1" @default.
- W2896852537 countsByYear W28968525372022 @default.
- W2896852537 crossrefType "journal-article" @default.
- W2896852537 hasAuthorship W2896852537A5028368441 @default.
- W2896852537 hasAuthorship W2896852537A5028839300 @default.
- W2896852537 hasAuthorship W2896852537A5039992789 @default.
- W2896852537 hasAuthorship W2896852537A5066222130 @default.
- W2896852537 hasAuthorship W2896852537A5089605955 @default.
- W2896852537 hasBestOaLocation W28968525372 @default.
- W2896852537 hasConcept C104317684 @default.
- W2896852537 hasConcept C123894998 @default.
- W2896852537 hasConcept C153911025 @default.
- W2896852537 hasConcept C159047783 @default.
- W2896852537 hasConcept C159654299 @default.
- W2896852537 hasConcept C2522874641 @default.
- W2896852537 hasConcept C2776151397 @default.
- W2896852537 hasConcept C2777212814 @default.
- W2896852537 hasConcept C2777773057 @default.
- W2896852537 hasConcept C2779026604 @default.
- W2896852537 hasConcept C2781140520 @default.
- W2896852537 hasConcept C40767141 @default.
- W2896852537 hasConcept C54355233 @default.
- W2896852537 hasConcept C86803240 @default.
- W2896852537 hasConceptScore W2896852537C104317684 @default.
- W2896852537 hasConceptScore W2896852537C123894998 @default.
- W2896852537 hasConceptScore W2896852537C153911025 @default.
- W2896852537 hasConceptScore W2896852537C159047783 @default.
- W2896852537 hasConceptScore W2896852537C159654299 @default.
- W2896852537 hasConceptScore W2896852537C2522874641 @default.
- W2896852537 hasConceptScore W2896852537C2776151397 @default.
- W2896852537 hasConceptScore W2896852537C2777212814 @default.
- W2896852537 hasConceptScore W2896852537C2777773057 @default.
- W2896852537 hasConceptScore W2896852537C2779026604 @default.
- W2896852537 hasConceptScore W2896852537C2781140520 @default.
- W2896852537 hasConceptScore W2896852537C40767141 @default.
- W2896852537 hasConceptScore W2896852537C54355233 @default.
- W2896852537 hasConceptScore W2896852537C86803240 @default.
- W2896852537 hasFunder F4320326565 @default.
- W2896852537 hasIssue "11" @default.
- W2896852537 hasLocation W28968525371 @default.
- W2896852537 hasLocation W28968525372 @default.
- W2896852537 hasLocation W28968525373 @default.
- W2896852537 hasLocation W28968525374 @default.
- W2896852537 hasOpenAccess W2896852537 @default.
- W2896852537 hasPrimaryLocation W28968525371 @default.
- W2896852537 hasRelatedWork W2075918933 @default.
- W2896852537 hasRelatedWork W2351417972 @default.
- W2896852537 hasRelatedWork W2357844006 @default.
- W2896852537 hasRelatedWork W2362309714 @default.
- W2896852537 hasRelatedWork W2371993564 @default.
- W2896852537 hasRelatedWork W2376988032 @default.
- W2896852537 hasRelatedWork W2399388923 @default.
- W2896852537 hasRelatedWork W2404261824 @default.
- W2896852537 hasRelatedWork W2431377509 @default.
- W2896852537 hasRelatedWork W2896852537 @default.
- W2896852537 hasVolume "8" @default.
- W2896852537 isParatext "false" @default.
- W2896852537 isRetracted "false" @default.
- W2896852537 magId "2896852537" @default.
- W2896852537 workType "article" @default.