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• W2896870800 abstract "ABSTRACT Synergistic interactions between macromolecular crowding and confinement spatially organize transcription and translation in cells. Yet, reproducing such spatial ordering in cell-free expression platforms has proven to be elusive. Here we report crowding- and confinement-driven spatial self-organization of cell-free expression that mimics expression behavior within and around the nucleoid of prokaryotes. These experiments use Ficoll-70 to approximate cellular macromolecular crowding conditions within cell-size lipid vesicles. Intriguingly, there was an abrupt change in transcriptional dynamics when crowding reached physiologically relevant levels. Imaging experiments revealed that this change in transcriptional dynamics was coincident with localization of plasmid DNA and mRNA at the vesicle wall. Computer simulations demonstrated that crowding leads to an entropically induced attraction between plasmid DNA and the wall, causing localization of DNA near the wall at sufficiently high crowding levels. The experiments demonstrate cell-like spatial organization of translation, where translational activity is controlled by chromosomally-templated positioning of mRNA. This cell-free system provides a flexible experimental platform to probe the underlying mechanisms of self-organization of membrane-less structures in cells and the spatial control of gene expression." @default.
• W2896870800 created "2018-10-26" @default.
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• W2896870800 date "2018-10-16" @default.
• W2896870800 modified "2023-10-07" @default.
• W2896870800 title "Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression" @default.
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• W2896870800 doi "https://doi.org/10.1101/445544" @default.
• W2896870800 hasPublicationYear "2018" @default.
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