FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896888626> ?p ?o ?g. }

• W2896888626 endingPage "640" @default.
• W2896888626 startingPage "630" @default.
• W2896888626 abstract "Abstract Colorectal cancer ( CRC ) remains both common and fatal, and its successful treatment is greatly limited by the development of stem cell‐like characteristics (stemness) and chemoresistance. MiR‐30‐5p has been shown to function as a tumor suppressor by targeting the Wnt/β‐catenin signaling pathway, but its activity in CRC has never been assessed. We hypothesized that miR‐30‐5p exerts anti‐oncogenic effects in CRC by regulating the USP 22/Wnt/β‐catenin signaling axis. In the present study, we demonstrate that tissues from CRC patients and human CRC cell lines show significantly decreased miR‐30‐5p family expression. After identifying the 3’ UTR of USP 22 as a potential binding site of miR‐30‐5p, we constructed a luciferase reporter containing the potential miR‐30‐5p binding site and measured the effects on USP 22 expression. Western blot assays showed that miR‐30‐5p decreased USP 22 protein expression in HEK 293 and Caco2 CRC cells. To evaluate the effects of miR‐30‐5p on CRC cell stemness, we isolated CD 133 + CRC cells (Caco2 and HCT 15). We then determined that, while miR‐30‐5p is normally decreased in CD 133 + CRC cells, miR‐30‐5p overexpression significantly reduces expression of stem cell markers CD 133 and Sox2, sphere formation, and cell proliferation. Similarly, we found that miR‐30‐5p expression is normally reduced in 5‐fluorouracil (5‐ FU ) resistant CRC cells, whereas miR‐30‐5p overexpression in 5‐ FU resistant cells reduces sphere formation and cell viability. Inhibition of miR‐30‐5p reversed the process. Finally, we determined that miR‐30‐5p attenuates the expression of Wnt/β‐catenin signaling target genes (Axin2 and MYC ), Wnt luciferase activity, and β‐catenin protein levels in CRC stem cells." @default.
• W2896888626 created "2018-10-26" @default.
• W2896888626 creator A5003046085 @default.
• W2896888626 creator A5003980543 @default.
• W2896888626 creator A5005582179 @default.
• W2896888626 creator A5049001572 @default.
• W2896888626 creator A5076832561 @default.
• W2896888626 creator A5078730435 @default.
• W2896888626 date "2018-10-19" @default.
• W2896888626 modified "2023-10-10" @default.
• W2896888626 title "MiR‐30‐5p suppresses cell chemoresistance and stemness in colorectal cancer through <scp>USP</scp>22/Wnt/β‐catenin signaling axis" @default.
• W2896888626 cites W1832060645 @default.
• W2896888626 cites W1862015311 @default.
• W2896888626 cites W1968631483 @default.
• W2896888626 cites W1969192247 @default.
• W2896888626 cites W1970539996 @default.
• W2896888626 cites W1989215255 @default.
• W2896888626 cites W2001258296 @default.
• W2896888626 cites W2023529679 @default.
• W2896888626 cites W2024711136 @default.
• W2896888626 cites W2028593825 @default.
• W2896888626 cites W2031348772 @default.
• W2896888626 cites W2035805452 @default.
• W2896888626 cites W2042970437 @default.
• W2896888626 cites W2051011734 @default.
• W2896888626 cites W2056204720 @default.
• W2896888626 cites W2062069198 @default.
• W2896888626 cites W2070856992 @default.
• W2896888626 cites W2084218374 @default.
• W2896888626 cites W2086809547 @default.
• W2896888626 cites W2089300758 @default.
• W2896888626 cites W2090636859 @default.
• W2896888626 cites W2102085111 @default.
• W2896888626 cites W2104517740 @default.
• W2896888626 cites W2119284420 @default.
• W2896888626 cites W2121368761 @default.
• W2896888626 cites W2123693778 @default.
• W2896888626 cites W2124589535 @default.
• W2896888626 cites W2130979840 @default.
• W2896888626 cites W2156214772 @default.
• W2896888626 cites W2158021558 @default.
• W2896888626 cites W2162790141 @default.
• W2896888626 cites W2165087323 @default.
• W2896888626 cites W2192016206 @default.
• W2896888626 cites W2262414037 @default.
• W2896888626 cites W2339596461 @default.
• W2896888626 cites W2346835588 @default.
• W2896888626 cites W2418794140 @default.
• W2896888626 cites W2513545722 @default.
• W2896888626 cites W2513687207 @default.
• W2896888626 cites W2549653752 @default.
• W2896888626 cites W2591609625 @default.
• W2896888626 cites W2594373254 @default.
• W2896888626 cites W2597632520 @default.
• W2896888626 cites W2599116602 @default.
• W2896888626 cites W2615072496 @default.
• W2896888626 cites W2800134017 @default.
• W2896888626 cites W4239939231 @default.
• W2896888626 doi "https://doi.org/10.1111/jcmm.13968" @default.
• W2896888626 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6307779" @default.
• W2896888626 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30338942" @default.
• W2896888626 hasPublicationYear "2018" @default.
• W2896888626 type Work @default.
• W2896888626 sameAs 2896888626 @default.
• W2896888626 citedByCount "55" @default.
• W2896888626 countsByYear W28968886262019 @default.
• W2896888626 countsByYear W28968886262020 @default.
• W2896888626 countsByYear W28968886262021 @default.
• W2896888626 countsByYear W28968886262022 @default.
• W2896888626 countsByYear W28968886262023 @default.
• W2896888626 crossrefType "journal-article" @default.
• W2896888626 hasAuthorship W2896888626A5003046085 @default.
• W2896888626 hasAuthorship W2896888626A5003980543 @default.
• W2896888626 hasAuthorship W2896888626A5005582179 @default.
• W2896888626 hasAuthorship W2896888626A5049001572 @default.
• W2896888626 hasAuthorship W2896888626A5076832561 @default.
• W2896888626 hasAuthorship W2896888626A5078730435 @default.
• W2896888626 hasBestOaLocation W28968886261 @default.
• W2896888626 hasConcept C10116572 @default.
• W2896888626 hasConcept C104317684 @default.
• W2896888626 hasConcept C121608353 @default.
• W2896888626 hasConcept C137620995 @default.
• W2896888626 hasConcept C145059251 @default.
• W2896888626 hasConcept C145103041 @default.
• W2896888626 hasConcept C174510640 @default.
• W2896888626 hasConcept C185592680 @default.
• W2896888626 hasConcept C2776415932 @default.
• W2896888626 hasConcept C2777933648 @default.
• W2896888626 hasConcept C2778265714 @default.
• W2896888626 hasConcept C28328180 @default.
• W2896888626 hasConcept C30145163 @default.
• W2896888626 hasConcept C502942594 @default.
• W2896888626 hasConcept C526805850 @default.
• W2896888626 hasConcept C53227056 @default.
• W2896888626 hasConcept C54355233 @default.
• W2896888626 hasConcept C55427017 @default.
• W2896888626 hasConcept C62112901 @default.
• W2896888626 hasConcept C62478195 @default.

• next