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- W2896907369 abstract "Secondary caries and degradation of hybrid layers are two major challenges in achieving durable resin-dentin bonds. The objectives of the present study were to investigate the effects of a 2% quaternary ammonium silane (QAS) cavity cleanser on bacteria impregnated into dentin blocks and the gelatinolytic activity of the hybrid layers.Microtensile bond strength was first performed to evaluate if the 2% QAS cavity cleanser adversely affected bond strength. For antibacterial testing, Streptococcus mutans and Actinomyces naeslundii were impregnated into dentin blocks, respectively, prior to the application of the cavity cleanser. Live/dead bacterial staining and colony-forming unit (CFU) counts were performed to evaluate their antibacterial effects. Gelatinolytic activity within the hybrid layers was directly examined using in-situ zymography. A double-fluorescence technique was used to examine interfacial permeability immediately after bonding.The cavity cleanser did not adversely affect the bond strength of the adhesives tested (p>0.05). Antibacterial testing indicated that 2% QAS significantly killed impregnated bacteria within the dentin blocks compared with control group (p<0.05), which was comparable with the antibacterial activity of 2% chlorhexidine (p>0.05). Hybrid layers pretreated with 2% QAS showed significant decrease in enzyme activity compared with control group. With the use of 2% QAS, relatively lower interfacial permeability was observed, compared with control group and 2% chlorhexidine (p<0.05).The present study developed a 2% QAS cavity cleanser that possesses combined antimicrobial and anti-proteolytic activities to extend the longevity of resin-dentin bonds." @default.
- W2896907369 created "2018-10-26" @default.
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- W2896907369 date "2018-12-01" @default.
- W2896907369 modified "2023-10-16" @default.
- W2896907369 title "Quaternary ammonium silane-based antibacterial and anti-proteolytic cavity cleanser" @default.
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- W2896907369 doi "https://doi.org/10.1016/j.dental.2018.10.001" @default.
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