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- W2896918722 abstract "The absence of tools for the genetic manipulation of C. pneumoniae has hampered research into all aspects of its biology. In this study, we established a novel reproducible method for C. pneumoniae transformation based on a plasmid shuttle vector system. We constructed a C. pneumoniae plasmid backbone shuttle vector, pRSGFPCAT-Cpn. The construct expresses the red-shifted green fluorescent protein (RSGFP) fused to chloramphenicol acetyltransferase in C. pneumoniae . C. pneumoniae transformants stably retained pRSGFPCAT-Cpn and expressed RSGFP in epithelial cells, even in the absence of chloramphenicol. The successful transformation in C. pneumoniae using pRSGFPCAT-Cpn will advance the field of chlamydial genetics and is a promising new approach to investigate gene functions in C. pneumoniae biology. In addition, we demonstrated that pRSGFPCAT-Cpn overcame the plasmid species barrier without the need for recombination with an endogenous plasmid, indicating the potential probability of horizontal chlamydial pathogenic gene transfer by plasmids between chlamydial species." @default.
- W2896918722 created "2018-10-26" @default.
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- W2896918722 date "2018-10-31" @default.
- W2896918722 modified "2023-10-03" @default.
- W2896918722 title "The Genetic Transformation of Chlamydia pneumoniae" @default.
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- W2896918722 doi "https://doi.org/10.1128/msphere.00412-18" @default.
- W2896918722 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6180227" @default.
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