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• W2896929167 abstract "CRISPR-Cas9 technology provides a simple way to introduce targeted mutations into mammalian cells to induce loss-of-function phenotypes. The CRISPR-Cas9 system has now successfully been applied for genetic screens in many cell types, providing a powerful tool for functional genomics with manifold applications. Genome-wide guide-RNA (gRNA) libraries allow facile generation of a pool of cells, each harboring a gene knockout mutation that can be used for the study of gene function, pathway analysis or the identification of genes required for cellular fitness. Furthermore, CRISPR genetic screens can be applied for the discovery of genes whose knockout sensitizes cells to drug treatments or mediates drug resistance. Here, we provide a detailed protocol discussing the necessary steps for the successful performance of pooled CRISPR-Cas9 screens." @default.
• W2896929167 created "2018-10-26" @default.
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• W2896929167 date "2018-10-16" @default.
• W2896929167 modified "2023-09-26" @default.
• W2896929167 title "Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells" @default.
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• W2896929167 doi "https://doi.org/10.1007/978-1-4939-8805-1_15" @default.
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