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• W2896935192 abstract "Abstract The robustness of a high-redox potential laccase has been enhanced by swapping its second cupredoxin domain with that from another fungal laccase, which introduced a pool of neutral mutations in the protein sequence without affecting enzyme functionality. The new laccase showed outstanding stability to temperature, pH (2–9) and to organic solvents, while maintaining the ability to oxidize high-redox potential substrates. By engineering the signal peptide, enzyme secretion levels in Saccharomyces cerevisiae were increased, which allowed to purify the engineered enzyme for further characterization. The purified domain-swap laccase presented higher activity in the presence of ethanol or methanol, superior half-lives at 50–70 °C, improved stability at acidic pH, and similar catalytic efficiency for DMP albeit a lower one for ABTS (due to a shift in optimum pH). A new N-glycosylation site and a putative new surface salt-bridge were evaluated as possible determinants for the improved stability by site-directed mutagenesis. Although neither seemed to be strictly responsible for the improved thermostability, the new salt bridge was found to notably contribute to the high stability of the swapped enzyme in a broad pH range. Finally, the application potential of the new laccase was demonstrated with the enzymatic treatment of kraft lignin, an industrially relevant lignin stream, at high temperature, neutral pH and short incubation times." @default.
• W2896935192 created "2018-10-26" @default.
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• W2896935192 date "2018-10-23" @default.
• W2896935192 modified "2023-10-17" @default.
• W2896935192 title "A highly stable laccase obtained by swapping the second cupredoxin domain" @default.
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• W2896935192 doi "https://doi.org/10.1038/s41598-018-34008-3" @default.
• W2896935192 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6199291" @default.
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