FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2896984864> ?p ?o ?g. }

• W2896984864 endingPage "5341" @default.
• W2896984864 startingPage "5319" @default.
• W2896984864 abstract "Inhibitory neuromuscular transmission (NMT) was compared in the internal anal sphincter (IAS) and rectum of the Cynomolgus monkey, an animal with high gene sequence identity to humans. Nitrergic NMT was present in both muscles while purinergic NMT was limited to the rectum and VIPergic NMT to the IAS. The profile for monkey IAS more closely resembles humans than rodents. In both muscles, SK3 channels were localized to PDGFRα+ cells that were closely associated with nNOS+ /VIP+ nerves. Gene expression levels of P2RY subtypes were the same in IAS and rectum while KCNN expression levels were very similar. SK3 channel activation and inhibition caused faster/greater changes in contractile activity in rectum than IAS. P2Y1 receptor activation inhibited contraction in rectum while increasing contraction in IAS. The absence of purinergic NMT in the IAS may be due to poor coupling between P2Y1 receptors and SK3 channels on PDGFRα+ cells.Inhibitory neuromuscular transmission (NMT) was compared in the internal anal sphincter (IAS) and rectum of the Cynomolgus monkey, an animal with a high gene sequence identity to humans. Electrical field stimulation produced nitric oxide synthase (NOS)-dependent contractile inhibition in both muscles whereas P2Y1-dependent purinergic NMT was restricted to rectum. An additional NOS-independent, α-chymotrypsin-sensitive component was identified in the IAS consistent with vasoactive intestinal peptide-ergic (VIPergic) NMT. Microelectrode recordings revealed slow NOS-dependent inhibitory junction potentials (IJPs) in both muscles and fast P2Y1-dependent IJPs in rectum. The basis for the difference in purinergic NMT was investigated. PDGFRα+ /SK3+ cells were closely aligned with nNOS+ /VIP+ neurons in both muscles. Gene expression of P2RY was the same in IAS and rectum (P2RY1>>P2RY2-14) while KCNN3 expression was 32% greater in rectum. The SK channel inhibitor apamin doubled contractile activity in rectum while having minimal effect in the IAS. Contractile inhibition elicited with the SK channel agonist CyPPA was five times faster in rectum than in the IAS. The P2Y1 receptor agonist MRS2365 inhibited contraction in rectum but increased contraction in the IAS. In conclusion, both the IAS and the rectum have nitrergic NMT whereas purinergic NMT is limited to rectum and VIPergic NMT to the IAS. The profile in monkey IAS more closely resembles that of humans than rodents. The lack of purinergic NMT in the IAS cannot be attributed to the absence of PDGFRα+ cells, P2Y1 receptors or SK3 channels. Rather, it appears to be due to poor coupling between P2Y1 receptors and SK3 channels on PDGFRα+ cells." @default.
• W2896984864 created "2018-10-26" @default.
• W2896984864 creator A5000221987 @default.
• W2896984864 creator A5012146158 @default.
• W2896984864 creator A5032283017 @default.
• W2896984864 creator A5054369632 @default.
• W2896984864 creator A5066254052 @default.
• W2896984864 date "2018-10-13" @default.
• W2896984864 modified "2023-09-23" @default.
• W2896984864 title "Comparison of inhibitory neuromuscular transmission in the <i>Cynomolgus</i> monkey IAS and rectum: special emphasis on differences in purinergic transmission" @default.
• W2896984864 cites W1507564046 @default.
• W2896984864 cites W1517980537 @default.
• W2896984864 cites W1520019314 @default.
• W2896984864 cites W1521470553 @default.
• W2896984864 cites W1526197329 @default.
• W2896984864 cites W1557003590 @default.
• W2896984864 cites W1561385461 @default.
• W2896984864 cites W1566099836 @default.
• W2896984864 cites W1567587795 @default.
• W2896984864 cites W1569474087 @default.
• W2896984864 cites W1584230389 @default.
• W2896984864 cites W1595190498 @default.
• W2896984864 cites W1760947721 @default.
• W2896984864 cites W1822257401 @default.
• W2896984864 cites W1841016992 @default.
• W2896984864 cites W1844756494 @default.
• W2896984864 cites W1875583344 @default.
• W2896984864 cites W1940693565 @default.
• W2896984864 cites W1967634114 @default.
• W2896984864 cites W1969545527 @default.
• W2896984864 cites W1976572358 @default.
• W2896984864 cites W1977708272 @default.
• W2896984864 cites W1980495162 @default.
• W2896984864 cites W1981046160 @default.
• W2896984864 cites W1985290415 @default.
• W2896984864 cites W1987784438 @default.
• W2896984864 cites W1994880376 @default.
• W2896984864 cites W1999199181 @default.
• W2896984864 cites W1999456682 @default.
• W2896984864 cites W2001563619 @default.
• W2896984864 cites W2007531288 @default.
• W2896984864 cites W2008600963 @default.
• W2896984864 cites W2009549854 @default.
• W2896984864 cites W2018059030 @default.
• W2896984864 cites W2020019271 @default.
• W2896984864 cites W2020045182 @default.
• W2896984864 cites W2021858120 @default.
• W2896984864 cites W2025590033 @default.
• W2896984864 cites W2026980272 @default.
• W2896984864 cites W2029100084 @default.
• W2896984864 cites W2029922422 @default.
• W2896984864 cites W2038236189 @default.
• W2896984864 cites W2039321923 @default.
• W2896984864 cites W2041898542 @default.
• W2896984864 cites W2041911471 @default.
• W2896984864 cites W2043822158 @default.
• W2896984864 cites W2049314578 @default.
• W2896984864 cites W2051909844 @default.
• W2896984864 cites W2055795786 @default.
• W2896984864 cites W2055823345 @default.
• W2896984864 cites W2057678490 @default.
• W2896984864 cites W2058423368 @default.
• W2896984864 cites W2061660949 @default.
• W2896984864 cites W2064524187 @default.
• W2896984864 cites W2068696291 @default.
• W2896984864 cites W2075124455 @default.
• W2896984864 cites W2076842345 @default.
• W2896984864 cites W2085644581 @default.
• W2896984864 cites W2087626203 @default.
• W2896984864 cites W2088876815 @default.
• W2896984864 cites W2089215588 @default.
• W2896984864 cites W2095138676 @default.
• W2896984864 cites W2096802675 @default.
• W2896984864 cites W2099590846 @default.
• W2896984864 cites W2102810548 @default.
• W2896984864 cites W2104416101 @default.
• W2896984864 cites W2108399405 @default.
• W2896984864 cites W2111152560 @default.
• W2896984864 cites W2113568840 @default.
• W2896984864 cites W2118150066 @default.
• W2896984864 cites W2121301418 @default.
• W2896984864 cites W2131289313 @default.
• W2896984864 cites W2133390674 @default.
• W2896984864 cites W2133583538 @default.
• W2896984864 cites W2147968484 @default.
• W2896984864 cites W2150381121 @default.
• W2896984864 cites W2152827122 @default.
• W2896984864 cites W2159271666 @default.
• W2896984864 cites W2164897112 @default.
• W2896984864 cites W2166359818 @default.
• W2896984864 cites W2184697282 @default.
• W2896984864 cites W2263139781 @default.
• W2896984864 cites W2292333311 @default.
• W2896984864 cites W2317666265 @default.
• W2896984864 cites W2413685260 @default.
• W2896984864 cites W2417602457 @default.
• W2896984864 cites W2472322602 @default.
• W2896984864 cites W2568409770 @default.

• next