Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897029973> ?p ?o ?g. }
- W2897029973 endingPage "2646" @default.
- W2897029973 startingPage "2640" @default.
- W2897029973 abstract "Short- and medium-chain fatty acids (SMCFA) are important platform chemicals currently produced from nonsustainable resources. The engineering of microbial cells to produce SMCFA, however, lacks high-throughput methods to screen for best performing cells. Here, we present the development of a whole-cell biosensor for easy and rapid detection of SMCFA. The biosensor is based on a multicopy yeast plasmid containing the SMCFA-responsive PDR12 promoter coupled to GFP as the reporter gene. The sensor detected hexanoic, heptanoic and octanoic acid over a linear range up to 2, 1.5, and 0.75 mM, respectively, but did not show a linear response to decanoic and dodecanoic acid. We validated the functionality of the biosensor with culture supernatants of a previously engineered Saccharomyces cerevisiae octanoic acid producer strain and derivatives thereof. The biosensor signal correlated strongly with the octanoic acid concentrations as determined by gas chromatography. Thus, this biosensor enables the high-throughput screening of SMCFA producers and has the potential to drastically speed up the engineering of diverse SMCFA producing cell factories." @default.
- W2897029973 created "2018-10-26" @default.
- W2897029973 creator A5044924800 @default.
- W2897029973 creator A5064656627 @default.
- W2897029973 creator A5069532370 @default.
- W2897029973 creator A5080549207 @default.
- W2897029973 creator A5085158543 @default.
- W2897029973 date "2018-10-19" @default.
- W2897029973 modified "2023-10-17" @default.
- W2897029973 title "A Yeast-Based Biosensor for Screening of Short- and Medium-Chain Fatty Acid Production" @default.
- W2897029973 cites W1695687829 @default.
- W2897029973 cites W1893995452 @default.
- W2897029973 cites W1947202234 @default.
- W2897029973 cites W1965841046 @default.
- W2897029973 cites W1994202064 @default.
- W2897029973 cites W2007622308 @default.
- W2897029973 cites W2013056827 @default.
- W2897029973 cites W2024180190 @default.
- W2897029973 cites W2038591876 @default.
- W2897029973 cites W2044811726 @default.
- W2897029973 cites W2078272501 @default.
- W2897029973 cites W2088257674 @default.
- W2897029973 cites W2092009348 @default.
- W2897029973 cites W2101700211 @default.
- W2897029973 cites W2103311813 @default.
- W2897029973 cites W2103586941 @default.
- W2897029973 cites W2115168068 @default.
- W2897029973 cites W2128651962 @default.
- W2897029973 cites W2131350482 @default.
- W2897029973 cites W2145213155 @default.
- W2897029973 cites W2157618287 @default.
- W2897029973 cites W2208196644 @default.
- W2897029973 cites W2316754462 @default.
- W2897029973 cites W2430522320 @default.
- W2897029973 cites W2502716066 @default.
- W2897029973 cites W2522890841 @default.
- W2897029973 cites W2589105005 @default.
- W2897029973 cites W2590738036 @default.
- W2897029973 cites W2594152625 @default.
- W2897029973 cites W2601537167 @default.
- W2897029973 cites W2616983714 @default.
- W2897029973 cites W2647716263 @default.
- W2897029973 cites W2745478934 @default.
- W2897029973 cites W2755366111 @default.
- W2897029973 cites W2775766311 @default.
- W2897029973 cites W2806954185 @default.
- W2897029973 doi "https://doi.org/10.1021/acssynbio.8b00309" @default.
- W2897029973 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30338986" @default.
- W2897029973 hasPublicationYear "2018" @default.
- W2897029973 type Work @default.
- W2897029973 sameAs 2897029973 @default.
- W2897029973 citedByCount "30" @default.
- W2897029973 countsByYear W28970299732019 @default.
- W2897029973 countsByYear W28970299732020 @default.
- W2897029973 countsByYear W28970299732021 @default.
- W2897029973 countsByYear W28970299732022 @default.
- W2897029973 countsByYear W28970299732023 @default.
- W2897029973 crossrefType "journal-article" @default.
- W2897029973 hasAuthorship W2897029973A5044924800 @default.
- W2897029973 hasAuthorship W2897029973A5064656627 @default.
- W2897029973 hasAuthorship W2897029973A5069532370 @default.
- W2897029973 hasAuthorship W2897029973A5080549207 @default.
- W2897029973 hasAuthorship W2897029973A5085158543 @default.
- W2897029973 hasBestOaLocation W28970299732 @default.
- W2897029973 hasConcept C10349863 @default.
- W2897029973 hasConcept C104317684 @default.
- W2897029973 hasConcept C119128265 @default.
- W2897029973 hasConcept C150194340 @default.
- W2897029973 hasConcept C160756335 @default.
- W2897029973 hasConcept C161733203 @default.
- W2897029973 hasConcept C168170006 @default.
- W2897029973 hasConcept C178790620 @default.
- W2897029973 hasConcept C185592680 @default.
- W2897029973 hasConcept C2776950135 @default.
- W2897029973 hasConcept C2777576037 @default.
- W2897029973 hasConcept C2778471300 @default.
- W2897029973 hasConcept C2779222958 @default.
- W2897029973 hasConcept C2779490540 @default.
- W2897029973 hasConcept C43617362 @default.
- W2897029973 hasConcept C51323132 @default.
- W2897029973 hasConcept C543025807 @default.
- W2897029973 hasConcept C55493867 @default.
- W2897029973 hasConceptScore W2897029973C10349863 @default.
- W2897029973 hasConceptScore W2897029973C104317684 @default.
- W2897029973 hasConceptScore W2897029973C119128265 @default.
- W2897029973 hasConceptScore W2897029973C150194340 @default.
- W2897029973 hasConceptScore W2897029973C160756335 @default.
- W2897029973 hasConceptScore W2897029973C161733203 @default.
- W2897029973 hasConceptScore W2897029973C168170006 @default.
- W2897029973 hasConceptScore W2897029973C178790620 @default.
- W2897029973 hasConceptScore W2897029973C185592680 @default.
- W2897029973 hasConceptScore W2897029973C2776950135 @default.
- W2897029973 hasConceptScore W2897029973C2777576037 @default.
- W2897029973 hasConceptScore W2897029973C2778471300 @default.
- W2897029973 hasConceptScore W2897029973C2779222958 @default.
- W2897029973 hasConceptScore W2897029973C2779490540 @default.
- W2897029973 hasConceptScore W2897029973C43617362 @default.
- W2897029973 hasConceptScore W2897029973C51323132 @default.