Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897030049> ?p ?o ?g. }
- W2897030049 endingPage "471" @default.
- W2897030049 startingPage "463" @default.
- W2897030049 abstract "Pyrogallol is naturally found in aquatic plant and has been proposed as a substrate of tyrosinase. In this study, we evaluated the dual effect of pyrogallol on tyrosinase as an inhibitor in the presence of L‑DOPA simultaneously via integrating methods of enzyme kinetics and computational molecular dynamics (MD) simulations. Pyrogallol was found to be a reversible inhibitor of tyrosinase in the presence of L‑DOPA and its induced mechanism was the parabolic non-competitive inhibition type (IC50 = 0.772 ± 0.003 mM and Ki = 0.529 ± 0.022 mM). Kinetic measurements by real-time interval assay showed that pyrogallol induced rapid inactivation process composing with slight activations at the low dose. Spectrofluorimetry studies showed that pyrogallol mainly induced regional changes in the active site of tyrosinase accompanying with hydrophobic disruption at high dose. The computational MD simulations further revealed that pyrogallol could interact with several residues near the tyrosinase active site pocket such as HIS61, HIS85, HIS259, ASN260, HIS263, VAL283, and ALA296. Our study provides insight into the mechanism by which hydroxyl group composing pyrogallol inhibit tyrosinase and pyrogallol is a potential natural anti-pigmentation agent." @default.
- W2897030049 created "2018-10-26" @default.
- W2897030049 creator A5002413587 @default.
- W2897030049 creator A5002823133 @default.
- W2897030049 creator A5015308460 @default.
- W2897030049 creator A5018341368 @default.
- W2897030049 creator A5030078271 @default.
- W2897030049 creator A5049362904 @default.
- W2897030049 creator A5052167362 @default.
- W2897030049 creator A5061210130 @default.
- W2897030049 date "2019-01-01" @default.
- W2897030049 modified "2023-10-16" @default.
- W2897030049 title "The inhibitory effect of pyrogallol on tyrosinase activity and structure: Integration study of inhibition kinetics with molecular dynamics simulation" @default.
- W2897030049 cites W161162682 @default.
- W2897030049 cites W1932344327 @default.
- W2897030049 cites W1948547705 @default.
- W2897030049 cites W1963924039 @default.
- W2897030049 cites W1964676912 @default.
- W2897030049 cites W1969401407 @default.
- W2897030049 cites W1972721556 @default.
- W2897030049 cites W1973866335 @default.
- W2897030049 cites W1974781567 @default.
- W2897030049 cites W1975920134 @default.
- W2897030049 cites W1977561692 @default.
- W2897030049 cites W1986133342 @default.
- W2897030049 cites W2003890193 @default.
- W2897030049 cites W2010068810 @default.
- W2897030049 cites W2010406620 @default.
- W2897030049 cites W2015728302 @default.
- W2897030049 cites W2016331364 @default.
- W2897030049 cites W2023243778 @default.
- W2897030049 cites W2036558654 @default.
- W2897030049 cites W2046817964 @default.
- W2897030049 cites W2047449164 @default.
- W2897030049 cites W2054364004 @default.
- W2897030049 cites W2057288775 @default.
- W2897030049 cites W2076908231 @default.
- W2897030049 cites W2079187998 @default.
- W2897030049 cites W2086230261 @default.
- W2897030049 cites W2091854427 @default.
- W2897030049 cites W2095256839 @default.
- W2897030049 cites W2113191844 @default.
- W2897030049 cites W2122219996 @default.
- W2897030049 cites W2128545587 @default.
- W2897030049 cites W2131368980 @default.
- W2897030049 cites W2132262459 @default.
- W2897030049 cites W2132629607 @default.
- W2897030049 cites W2150225724 @default.
- W2897030049 cites W2158113006 @default.
- W2897030049 cites W2162494862 @default.
- W2897030049 cites W2470561160 @default.
- W2897030049 cites W2481600564 @default.
- W2897030049 cites W2485868818 @default.
- W2897030049 cites W2524139724 @default.
- W2897030049 cites W2551495003 @default.
- W2897030049 cites W2553910164 @default.
- W2897030049 cites W2559881503 @default.
- W2897030049 cites W2559945265 @default.
- W2897030049 cites W2560561137 @default.
- W2897030049 cites W2596423906 @default.
- W2897030049 cites W2912179861 @default.
- W2897030049 cites W4233743176 @default.
- W2897030049 doi "https://doi.org/10.1016/j.ijbiomac.2018.10.046" @default.
- W2897030049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30326223" @default.
- W2897030049 hasPublicationYear "2019" @default.
- W2897030049 type Work @default.
- W2897030049 sameAs 2897030049 @default.
- W2897030049 citedByCount "23" @default.
- W2897030049 countsByYear W28970300492019 @default.
- W2897030049 countsByYear W28970300492020 @default.
- W2897030049 countsByYear W28970300492021 @default.
- W2897030049 countsByYear W28970300492022 @default.
- W2897030049 countsByYear W28970300492023 @default.
- W2897030049 crossrefType "journal-article" @default.
- W2897030049 hasAuthorship W2897030049A5002413587 @default.
- W2897030049 hasAuthorship W2897030049A5002823133 @default.
- W2897030049 hasAuthorship W2897030049A5015308460 @default.
- W2897030049 hasAuthorship W2897030049A5018341368 @default.
- W2897030049 hasAuthorship W2897030049A5030078271 @default.
- W2897030049 hasAuthorship W2897030049A5049362904 @default.
- W2897030049 hasAuthorship W2897030049A5052167362 @default.
- W2897030049 hasAuthorship W2897030049A5061210130 @default.
- W2897030049 hasConcept C121332964 @default.
- W2897030049 hasConcept C12554922 @default.
- W2897030049 hasConcept C147597530 @default.
- W2897030049 hasConcept C148898269 @default.
- W2897030049 hasConcept C181199279 @default.
- W2897030049 hasConcept C185592680 @default.
- W2897030049 hasConcept C202751555 @default.
- W2897030049 hasConcept C2776344562 @default.
- W2897030049 hasConcept C2777752497 @default.
- W2897030049 hasConcept C41183919 @default.
- W2897030049 hasConcept C55493867 @default.
- W2897030049 hasConcept C59593255 @default.
- W2897030049 hasConcept C62520636 @default.
- W2897030049 hasConcept C71240020 @default.
- W2897030049 hasConcept C86803240 @default.
- W2897030049 hasConcept C87554066 @default.