FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897052235> ?p ?o ?g. }

• W2897052235 endingPage "e0205905" @default.
• W2897052235 startingPage "e0205905" @default.
• W2897052235 abstract "Homeoproteins contain the conserved homeodomain (HD) and have an important role determining embryo body plan during development. HDs increase their DNA-binding specificity by interacting with additional cofactors outlining a Hox interactome with a multiplicity of protein-protein interactions. In Drosophila, the first link of functional contact with a general transcription factor (GTF) was found between Antennapedia (Antp) and BIP2 (TFIID complex). Hox proteins also interact with other components of Pol II machinery such as the subunit Med19 from Mediator (MED) complex, TFIIEβ and transcription-pausing factor M1BP. All these interactions clearly demonstrate Hox-driven transcriptional regulation, but the precise molecular mechanism remains unclear. In this paper, we focused on the Antp-TFIIEβ protein-protein interface to establish the specific contacts as well as its functional role. Using Bimolecular Fluorescence Complementation (BiFC) in cell culture and in vivo we found that TFIIEβ interacts with Antp through the HD independently of the YPWM motif and the direct physical interaction is at helix 2, specifically aminoacidic positions I32 and H36 of Antp. We also found, through ectopic assays, that these two positions in helix 2 are crucial for Antp homeotic function in head involution, and thoracic and antenna-to tarsus transformations. Interestingly, overexpression of Antp and TFIIEβ in the antennal disc showed that this interaction is required for the antenna-to-tarsus transformation. In conclusion, interaction of Antp with TFIIEβ is important for the functional specificity of Antennapedia, and amino acids 32 and 36 in Antp HD helix 2 are key for this interaction. Our results open the possibility to more broadly analyze Antp-TFIIEβ interaction on the transcriptional control for the activation and/or repression of target genes in the Hox interactome during Drosophila development." @default.
• W2897052235 created "2018-10-26" @default.
• W2897052235 creator A5004586530 @default.
• W2897052235 creator A5007762850 @default.
• W2897052235 creator A5027760691 @default.
• W2897052235 creator A5057155991 @default.
• W2897052235 creator A5090364483 @default.
• W2897052235 date "2018-10-15" @default.
• W2897052235 modified "2023-10-18" @default.
• W2897052235 title "Transcription factor TFIIEβ interacts with two exposed positions in helix 2 of the Antennapedia homeodomain to control homeotic function in Drosophila" @default.
• W2897052235 cites W1584683177 @default.
• W2897052235 cites W159147729 @default.
• W2897052235 cites W1664008877 @default.
• W2897052235 cites W1843992786 @default.
• W2897052235 cites W1854018284 @default.
• W2897052235 cites W1967485212 @default.
• W2897052235 cites W1975538460 @default.
• W2897052235 cites W1977175239 @default.
• W2897052235 cites W1978637982 @default.
• W2897052235 cites W1985441572 @default.
• W2897052235 cites W1987349623 @default.
• W2897052235 cites W1990342419 @default.
• W2897052235 cites W1994664042 @default.
• W2897052235 cites W2000743500 @default.
• W2897052235 cites W2000763715 @default.
• W2897052235 cites W2003999527 @default.
• W2897052235 cites W2006788765 @default.
• W2897052235 cites W2009547655 @default.
• W2897052235 cites W2013638181 @default.
• W2897052235 cites W2035378222 @default.
• W2897052235 cites W2070370394 @default.
• W2897052235 cites W2079626024 @default.
• W2897052235 cites W2093267013 @default.
• W2897052235 cites W2109434173 @default.
• W2897052235 cites W2126049906 @default.
• W2897052235 cites W2136937190 @default.
• W2897052235 cites W2138109258 @default.
• W2897052235 cites W2167379325 @default.
• W2897052235 cites W2533428853 @default.
• W2897052235 cites W2752770712 @default.
• W2897052235 doi "https://doi.org/10.1371/journal.pone.0205905" @default.
• W2897052235 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6188894" @default.
• W2897052235 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30321227" @default.
• W2897052235 hasPublicationYear "2018" @default.
• W2897052235 type Work @default.
• W2897052235 sameAs 2897052235 @default.
• W2897052235 citedByCount "4" @default.
• W2897052235 countsByYear W28970522352020 @default.
• W2897052235 countsByYear W28970522352021 @default.
• W2897052235 countsByYear W28970522352022 @default.
• W2897052235 crossrefType "journal-article" @default.
• W2897052235 hasAuthorship W2897052235A5004586530 @default.
• W2897052235 hasAuthorship W2897052235A5007762850 @default.
• W2897052235 hasAuthorship W2897052235A5027760691 @default.
• W2897052235 hasAuthorship W2897052235A5057155991 @default.
• W2897052235 hasAuthorship W2897052235A5090364483 @default.
• W2897052235 hasBestOaLocation W28970522351 @default.
• W2897052235 hasConcept C101762097 @default.
• W2897052235 hasConcept C102622118 @default.
• W2897052235 hasConcept C104317684 @default.
• W2897052235 hasConcept C111039654 @default.
• W2897052235 hasConcept C121587040 @default.
• W2897052235 hasConcept C150194340 @default.
• W2897052235 hasConcept C188740287 @default.
• W2897052235 hasConcept C2780172944 @default.
• W2897052235 hasConcept C44498088 @default.
• W2897052235 hasConcept C46751350 @default.
• W2897052235 hasConcept C54355233 @default.
• W2897052235 hasConcept C86339819 @default.
• W2897052235 hasConcept C86803240 @default.
• W2897052235 hasConcept C95444343 @default.
• W2897052235 hasConceptScore W2897052235C101762097 @default.
• W2897052235 hasConceptScore W2897052235C102622118 @default.
• W2897052235 hasConceptScore W2897052235C104317684 @default.
• W2897052235 hasConceptScore W2897052235C111039654 @default.
• W2897052235 hasConceptScore W2897052235C121587040 @default.
• W2897052235 hasConceptScore W2897052235C150194340 @default.
• W2897052235 hasConceptScore W2897052235C188740287 @default.
• W2897052235 hasConceptScore W2897052235C2780172944 @default.
• W2897052235 hasConceptScore W2897052235C44498088 @default.
• W2897052235 hasConceptScore W2897052235C46751350 @default.
• W2897052235 hasConceptScore W2897052235C54355233 @default.
• W2897052235 hasConceptScore W2897052235C86339819 @default.
• W2897052235 hasConceptScore W2897052235C86803240 @default.
• W2897052235 hasConceptScore W2897052235C95444343 @default.
• W2897052235 hasFunder F4320321739 @default.
• W2897052235 hasIssue "10" @default.
• W2897052235 hasLocation W28970522351 @default.
• W2897052235 hasLocation W28970522352 @default.
• W2897052235 hasLocation W28970522353 @default.
• W2897052235 hasLocation W28970522354 @default.
• W2897052235 hasLocation W28970522355 @default.
• W2897052235 hasLocation W28970522356 @default.
• W2897052235 hasOpenAccess W2897052235 @default.
• W2897052235 hasPrimaryLocation W28970522351 @default.
• W2897052235 hasRelatedWork W1830151389 @default.
• W2897052235 hasRelatedWork W1869718192 @default.
• W2897052235 hasRelatedWork W1997497353 @default.

• next