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- W2897062281 endingPage "e0205274" @default.
- W2897062281 startingPage "e0205274" @default.
- W2897062281 abstract "Glutathione S‒transferases (GSTs) are multifunctional enzymes that play an important role in detoxification, cellular signalling, and the stress response. Camelus dromedarius is well-adapted to survive in extreme desert climate and it has GSTs, for which limited information is available. This study investigated the structure-function and thermodynamic properties of a mu-class camel GST (CdGSTM1) at different pH. Recombinant CdGSTM1 (25.7 kDa) was expressed in E. coli and purified to homogeneity. Dimeric CdGSTM1 dissociated into stable but inactive monomeric subunits at low pH. Conformational and thermodynamic changes during the thermal unfolding pathway of dimeric and monomeric CdGSTM1 were characterised via a thermal shift assay and dynamic multimode spectroscopy (DMS). The thermal shift assay based on intrinsic tryptophan fluorescence revealed that CdGSTM1 underwent a two-state unfolding pathway at pH 1.0-10.0. Its Tm value varied with varying pH. Another orthogonal technique based on far-UV CD also exhibited two-state unfolding in the dimeric and monomeric states. Generally, proteins tend to lose structural integrity and stability at low pH; however, monomeric CdGSTM1 at pH 2.0 was thermally more stable and unfolded with lower van't Hoff enthalpy. The present findings provide essential information regarding the structural, functional, and thermodynamic properties of CdGSTM1 at pH 1.0-10.0." @default.
- W2897062281 created "2018-10-26" @default.
- W2897062281 creator A5011469782 @default.
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- W2897062281 date "2018-10-10" @default.
- W2897062281 modified "2023-10-12" @default.
- W2897062281 title "Monomeric Camelus dromedarius GSTM1 at low pH is structurally more thermostable than its native dimeric form" @default.
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- W2897062281 cites W1718991191 @default.
- W2897062281 cites W1963629833 @default.
- W2897062281 cites W1969419384 @default.
- W2897062281 cites W1972937447 @default.
- W2897062281 cites W1973128833 @default.
- W2897062281 cites W1975986737 @default.
- W2897062281 cites W1977138583 @default.
- W2897062281 cites W1978041454 @default.
- W2897062281 cites W1978790337 @default.
- W2897062281 cites W1985494098 @default.
- W2897062281 cites W1989239683 @default.
- W2897062281 cites W1994025158 @default.
- W2897062281 cites W1999068901 @default.
- W2897062281 cites W2002331462 @default.
- W2897062281 cites W2002934299 @default.
- W2897062281 cites W2013534907 @default.
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- W2897062281 cites W2039031002 @default.
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- W2897062281 cites W2046750894 @default.
- W2897062281 cites W2047822026 @default.
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- W2897062281 cites W2085728428 @default.
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- W2897062281 cites W2324614614 @default.
- W2897062281 cites W2328924545 @default.
- W2897062281 cites W2340400496 @default.
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- W2897062281 doi "https://doi.org/10.1371/journal.pone.0205274" @default.
- W2897062281 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6179282" @default.
- W2897062281 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30303997" @default.
- W2897062281 hasPublicationYear "2018" @default.
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