FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897066502> ?p ?o ?g. }

• W2897066502 abstract "Plasmodium falciparum exhibits a high degree of inter-isolate genetic diversity in its variant surface antigen (VSA) families: P. falciparum erythrocyte membrane protein 1, repetitive interspersed family (RIFIN) and subtelomeric variable open reading frame (STEVOR). The role of recombination for the generation of this diversity is a subject of ongoing research. Here the genome of E5, a sibling of the 3D7 genome strain is presented. Short and long read whole genome sequencing (WGS) techniques (Ilumina, Pacific Bioscience) and a set of 84 microsatellites (MS) were employed to characterize the 3D7 and non-3D7 parts of the E5 genome. This is the first time that VSA genes in sibling parasites were analysed with long read sequencing technology.Of the 5733 E5 genes only 278 genes, mostly var and rifin/stevor genes, had no orthologues in the 3D7 genome. WGS and MS analysis revealed that chromosomal crossovers occurred at a rate of 0-3 per chromosome. var, stevor and rifin genes were inherited within the respective non-3D7 or 3D7 chromosomal context. 54 of the 84 MS PCR fragments correctly identified the respective MS as 3D7- or non-3D7 and this correlated with var and rifin/stevor gene inheritance in the adjacent chromosomal regions. E5 had 61 var and 189 rifin/stevor genes. One large non-chromosomal recombination event resulted in a new var gene on chromosome 14. The remainder of the E5 3D7-type subtelomeric and central regions were identical to 3D7.The data show that the rifin/stevor and var gene families represent the most diverse compartments of the P. falciparum genome but that the majority of var genes are inherited without alterations within their respective parental chromosomal context. Furthermore, MS genotyping with 54 MS can successfully distinguish between two sibling progeny of a natural P. falciparum cross and thus can be used to investigate identity by descent in field isolates." @default.
• W2897066502 created "2018-10-26" @default.
• W2897066502 creator A5009000244 @default.
• W2897066502 creator A5012004257 @default.
• W2897066502 creator A5082535106 @default.
• W2897066502 date "2018-10-22" @default.
• W2897066502 modified "2023-10-13" @default.
• W2897066502 title "Whole genome sequencing and microsatellite analysis of the Plasmodium falciparum E5 NF54 strain show that the var, rifin and stevor gene families follow Mendelian inheritance" @default.
• W2897066502 cites W1485626074 @default.
• W2897066502 cites W1545164994 @default.
• W2897066502 cites W1889956545 @default.
• W2897066502 cites W1944863757 @default.
• W2897066502 cites W1964782012 @default.
• W2897066502 cites W1966447624 @default.
• W2897066502 cites W1966553905 @default.
• W2897066502 cites W1970110423 @default.
• W2897066502 cites W1977845940 @default.
• W2897066502 cites W1978734850 @default.
• W2897066502 cites W1981873171 @default.
• W2897066502 cites W1984347606 @default.
• W2897066502 cites W1984669077 @default.
• W2897066502 cites W1985706362 @default.
• W2897066502 cites W2008856488 @default.
• W2897066502 cites W2012787238 @default.
• W2897066502 cites W2019851585 @default.
• W2897066502 cites W2020489688 @default.
• W2897066502 cites W2021874649 @default.
• W2897066502 cites W2027834647 @default.
• W2897066502 cites W2047817180 @default.
• W2897066502 cites W2049700891 @default.
• W2897066502 cites W2050052884 @default.
• W2897066502 cites W2058631304 @default.
• W2897066502 cites W2068404794 @default.
• W2897066502 cites W2072975902 @default.
• W2897066502 cites W2075935548 @default.
• W2897066502 cites W2076050649 @default.
• W2897066502 cites W2078109159 @default.
• W2897066502 cites W2087628191 @default.
• W2897066502 cites W2099333915 @default.
• W2897066502 cites W2107646820 @default.
• W2897066502 cites W2112373545 @default.
• W2897066502 cites W2113826143 @default.
• W2897066502 cites W2114390237 @default.
• W2897066502 cites W2117132127 @default.
• W2897066502 cites W2129159324 @default.
• W2897066502 cites W2130078103 @default.
• W2897066502 cites W2134917335 @default.
• W2897066502 cites W2135697415 @default.
• W2897066502 cites W2146556804 @default.
• W2897066502 cites W2149050959 @default.
• W2897066502 cites W2156273926 @default.
• W2897066502 cites W2158160080 @default.
• W2897066502 cites W2158297569 @default.
• W2897066502 cites W2162928486 @default.
• W2897066502 cites W2165672288 @default.
• W2897066502 cites W2168133698 @default.
• W2897066502 cites W2168200296 @default.
• W2897066502 cites W2170488656 @default.
• W2897066502 cites W2171787842 @default.
• W2897066502 cites W2193578681 @default.
• W2897066502 cites W2202810729 @default.
• W2897066502 cites W2234728088 @default.
• W2897066502 cites W2264802137 @default.
• W2897066502 cites W2338978258 @default.
• W2897066502 cites W2403085872 @default.
• W2897066502 cites W2463298498 @default.
• W2897066502 cites W2467407056 @default.
• W2897066502 cites W2514285416 @default.
• W2897066502 cites W2559282936 @default.
• W2897066502 cites W2743501483 @default.
• W2897066502 cites W2766207587 @default.
• W2897066502 cites W2787455165 @default.
• W2897066502 cites W2801980266 @default.
• W2897066502 doi "https://doi.org/10.1186/s12936-018-2503-2" @default.
• W2897066502 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6198375" @default.
• W2897066502 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30348135" @default.
• W2897066502 hasPublicationYear "2018" @default.
• W2897066502 type Work @default.
• W2897066502 sameAs 2897066502 @default.
• W2897066502 citedByCount "5" @default.
• W2897066502 countsByYear W28970665022019 @default.
• W2897066502 countsByYear W28970665022020 @default.
• W2897066502 countsByYear W28970665022022 @default.
• W2897066502 countsByYear W28970665022023 @default.
• W2897066502 crossrefType "journal-article" @default.
• W2897066502 hasAuthorship W2897066502A5009000244 @default.
• W2897066502 hasAuthorship W2897066502A5012004257 @default.
• W2897066502 hasAuthorship W2897066502A5082535106 @default.
• W2897066502 hasBestOaLocation W28970665021 @default.
• W2897066502 hasConcept C104317684 @default.
• W2897066502 hasConcept C141231307 @default.
• W2897066502 hasConcept C180754005 @default.
• W2897066502 hasConcept C23069440 @default.
• W2897066502 hasConcept C24432333 @default.
• W2897066502 hasConcept C30481170 @default.
• W2897066502 hasConcept C34957205 @default.
• W2897066502 hasConcept C36857842 @default.
• W2897066502 hasConcept C54355233 @default.
• W2897066502 hasConcept C61320498 @default.
• W2897066502 hasConcept C86803240 @default.

• next