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• W2897087034 abstract "In this study, the preventive effects of NGF against colistin-induced autophagy and apoptosis in PC12 cells have been investigated. Fluorescence microscopy, real-time PCR, transmission electron microscopy (TEM), flow cytometery, and western blotting technique were used. The results showed that large amounts of autophagosomes and apoptotic markers were triggered by colistin. Consistently, a significant increase has been noted at mRNA and protein levels in autophagy and apoptosis-related genes. Besides, TEM analysis showed that autophagic vacuoles were obvious at 12 h, while nuclear chromatin condensation and edge accumulation were clearly seen at 24 h in colistin alone group. Importantly, the visual autophagy and apoptosis were markedly reduced with NGF treatment in a dose-dependent manner. Moreover, colistin-induced reduction in mitochondrial membrane potential was partly attenuated by NGF in a dose dependent manner. In summary, NGF ameliorated colistin-induced apoptosis and autophagy, and partially recovered MMP in PC12 cells." @default.
• W2897087034 created "2018-10-26" @default.
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• W2897087034 date "2018-11-21" @default.
• W2897087034 modified "2023-10-10" @default.
• W2897087034 title "Preventive effects of nerve growth factor against colistin-induced autophagy and apoptosis in PC12 cells" @default.
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• W2897087034 doi "https://doi.org/10.1080/15376516.2018.1534298" @default.
• W2897087034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30318960" @default.
• W2897087034 hasPublicationYear "2018" @default.
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