Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897208443> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2897208443 endingPage "S979" @default.
- W2897208443 startingPage "S979" @default.
- W2897208443 abstract "Molecular analysis of cf-DNA in NSCLC patients enables detection and monitoring of EGFR mutations. It allows for detection of the acquired resistance for 1st and 2nd generation of EGFR-TKIs caused Thr790Met substitution. The third generation of EGFR-TKIs (osimertinib) could overcome the resistance in patients with Thr790Met mutation. The studied group included 23 Caucasian patients (8 male and 15 female, median age 71±9) with diagnosed lung adenocarcinoma and with EGFR mutations detected in tumor samples. Blood samples were collected before administration of EGFR-TKIs in all patients, and re-collected repeatedly from 10 patients during therapy. EGFR mutations and content of mutated cf-DNA were analyzed using ctEGFR Mutation Analysis Kit (Entrogen, USA) in Rotor-Gene Real-Time PCR device (Qiagen, Germany). Analysis of EGFR activating mutations in cf-DNA showed 82.61% concordance/sensitivity (19/23) with tumor samples. The mean content of mutated cf-DNA was 7.44% and it was significantly lower (p<0.000035) than in tumor samples (34.54%). Concentration of mutated cf-DNA positively correlated with advanced stage of the disease. Monitoring of EGFR status in cf-DNA showed reduction and stabilization of mutant DNA content with the emergence of response to EGFR-TKIs treatment. Primary Thr790Met substitution was undetectable in cf-DNA and in tumor samples. Acquired resistance in Thr790Met mechanism during EGFR-TKIs treatment was detected only in one patient (1/10). This patient responded to osimertinib therapy. Analysis of EGFR mutations in cf-DNA shows lower sensitivity than in DNA isolated from tumor samples. Multiple evaluations of EGFR status may be useful in monitoring of therapy and allows for early detection of acquired resistance." @default.
- W2897208443 created "2018-10-26" @default.
- W2897208443 creator A5009030600 @default.
- W2897208443 creator A5015309335 @default.
- W2897208443 creator A5021569660 @default.
- W2897208443 creator A5023250116 @default.
- W2897208443 creator A5023626766 @default.
- W2897208443 creator A5026187071 @default.
- W2897208443 creator A5029142660 @default.
- W2897208443 creator A5035358422 @default.
- W2897208443 creator A5044147183 @default.
- W2897208443 creator A5051885027 @default.
- W2897208443 creator A5064927276 @default.
- W2897208443 creator A5065723854 @default.
- W2897208443 creator A5079664149 @default.
- W2897208443 creator A5087508872 @default.
- W2897208443 creator A5087890182 @default.
- W2897208443 date "2018-10-01" @default.
- W2897208443 modified "2023-10-03" @default.
- W2897208443 title "P3.13-08 Assessment of EGFR Gene Mutations In cf-DNA in Monitoring of Response to EGFR TKIs in Patients with Lung Adenocarcinoma" @default.
- W2897208443 doi "https://doi.org/10.1016/j.jtho.2018.08.1848" @default.
- W2897208443 hasPublicationYear "2018" @default.
- W2897208443 type Work @default.
- W2897208443 sameAs 2897208443 @default.
- W2897208443 citedByCount "0" @default.
- W2897208443 crossrefType "journal-article" @default.
- W2897208443 hasAuthorship W2897208443A5009030600 @default.
- W2897208443 hasAuthorship W2897208443A5015309335 @default.
- W2897208443 hasAuthorship W2897208443A5021569660 @default.
- W2897208443 hasAuthorship W2897208443A5023250116 @default.
- W2897208443 hasAuthorship W2897208443A5023626766 @default.
- W2897208443 hasAuthorship W2897208443A5026187071 @default.
- W2897208443 hasAuthorship W2897208443A5029142660 @default.
- W2897208443 hasAuthorship W2897208443A5035358422 @default.
- W2897208443 hasAuthorship W2897208443A5044147183 @default.
- W2897208443 hasAuthorship W2897208443A5051885027 @default.
- W2897208443 hasAuthorship W2897208443A5064927276 @default.
- W2897208443 hasAuthorship W2897208443A5065723854 @default.
- W2897208443 hasAuthorship W2897208443A5079664149 @default.
- W2897208443 hasAuthorship W2897208443A5087508872 @default.
- W2897208443 hasAuthorship W2897208443A5087890182 @default.
- W2897208443 hasBestOaLocation W28972084431 @default.
- W2897208443 hasConcept C104317684 @default.
- W2897208443 hasConcept C121608353 @default.
- W2897208443 hasConcept C126322002 @default.
- W2897208443 hasConcept C143065580 @default.
- W2897208443 hasConcept C153911025 @default.
- W2897208443 hasConcept C160798450 @default.
- W2897208443 hasConcept C176944494 @default.
- W2897208443 hasConcept C2775999482 @default.
- W2897208443 hasConcept C2776256026 @default.
- W2897208443 hasConcept C2777626846 @default.
- W2897208443 hasConcept C2781182431 @default.
- W2897208443 hasConcept C501734568 @default.
- W2897208443 hasConcept C502942594 @default.
- W2897208443 hasConcept C50524450 @default.
- W2897208443 hasConcept C54355233 @default.
- W2897208443 hasConcept C552990157 @default.
- W2897208443 hasConcept C71924100 @default.
- W2897208443 hasConcept C86803240 @default.
- W2897208443 hasConceptScore W2897208443C104317684 @default.
- W2897208443 hasConceptScore W2897208443C121608353 @default.
- W2897208443 hasConceptScore W2897208443C126322002 @default.
- W2897208443 hasConceptScore W2897208443C143065580 @default.
- W2897208443 hasConceptScore W2897208443C153911025 @default.
- W2897208443 hasConceptScore W2897208443C160798450 @default.
- W2897208443 hasConceptScore W2897208443C176944494 @default.
- W2897208443 hasConceptScore W2897208443C2775999482 @default.
- W2897208443 hasConceptScore W2897208443C2776256026 @default.
- W2897208443 hasConceptScore W2897208443C2777626846 @default.
- W2897208443 hasConceptScore W2897208443C2781182431 @default.
- W2897208443 hasConceptScore W2897208443C501734568 @default.
- W2897208443 hasConceptScore W2897208443C502942594 @default.
- W2897208443 hasConceptScore W2897208443C50524450 @default.
- W2897208443 hasConceptScore W2897208443C54355233 @default.
- W2897208443 hasConceptScore W2897208443C552990157 @default.
- W2897208443 hasConceptScore W2897208443C71924100 @default.
- W2897208443 hasConceptScore W2897208443C86803240 @default.
- W2897208443 hasIssue "10" @default.
- W2897208443 hasLocation W28972084431 @default.
- W2897208443 hasOpenAccess W2897208443 @default.
- W2897208443 hasPrimaryLocation W28972084431 @default.
- W2897208443 hasRelatedWork W1567083771 @default.
- W2897208443 hasRelatedWork W1897859244 @default.
- W2897208443 hasRelatedWork W1985301420 @default.
- W2897208443 hasRelatedWork W2024850895 @default.
- W2897208443 hasRelatedWork W2147466517 @default.
- W2897208443 hasRelatedWork W2353108337 @default.
- W2897208443 hasRelatedWork W2968141797 @default.
- W2897208443 hasRelatedWork W3200167754 @default.
- W2897208443 hasRelatedWork W3209227928 @default.
- W2897208443 hasRelatedWork W4200199220 @default.
- W2897208443 hasVolume "13" @default.
- W2897208443 isParatext "false" @default.
- W2897208443 isRetracted "false" @default.
- W2897208443 magId "2897208443" @default.
- W2897208443 workType "article" @default.