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- W2897217434 abstract "A fluorescent biosensing strategy based on three helix molecular beacons (tMBs) for simultaneous simple and rapid detection of HIV related genes was developed. This tMBs contain outer loop sequences and fluorescent probes. The outer loop sequences consist of target capture sequences (cDNA) and the stems can lock the fluorescent probes (P). Initially, the probes were tethered to display weak fluorescence signals in the tMBs. Once the target DNAs opened loops, the stems of tMBs were dehybridized which resulted in turning on the fluorescence. By applying tMBs fluorescent switch detection strategy, the fluorescence response at 484 nm and 367 nm were corresponding to thioflavin (THT) and 2-aminopurine (2-AP) respectively with high selectivity and sensitivity. The detection limits were calculated to be 227 pM for HIV-1 and 352 pM for HIV-2, respectively. As a general detection model for multiple species, the proposed strategy exhibits outstanding analytical performance such as simple, low-cost, and high anti-interference capability, which provides a new and ultrasensitive biosensing platform for multiple DNAs simultaneous detection in biomedical research and early clinical diagnosis." @default.
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- W2897217434 date "2019-02-01" @default.
- W2897217434 modified "2023-10-15" @default.
- W2897217434 title "Functional three helix molecular beacon fluorescent “turn-on” probe for simple and sensitive simultaneous detection of two HIV DNAs" @default.
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- W2897217434 doi "https://doi.org/10.1016/j.snb.2018.10.110" @default.
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