Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897296970> ?p ?o ?g. }
- W2897296970 abstract "Genetic manipulation of the human malaria parasite Plasmodium falciparum is needed to explore pathogen biology and evaluate antimalarial targets. It is, however, aggravated by a low transfection efficiency, a paucity of selectable markers and a biased A/T-rich genome. While various enabling technologies have been introduced over the past two decades, facile and broad-range modification of essential genes remains challenging. We recently devised a new application of the Bxb1 integrase strategy to meet this need through an intronic attB sequence within the gene of interest. Although this attB is silent and without effect on intron splicing or protein translation and function, it allows efficient gene modification with minimal risk of unwanted changes at other genomic sites. We describe the range of applications for this new method as well as specific cases where it is preferred over CRISPR-Cas9 and other technologies. The advantages and limitations of various strategies for endogenous gene editing are also discussed." @default.
- W2897296970 created "2018-10-26" @default.
- W2897296970 creator A5046001598 @default.
- W2897296970 creator A5085765149 @default.
- W2897296970 date "2018-10-17" @default.
- W2897296970 modified "2023-10-17" @default.
- W2897296970 title "Diverse target gene modifications in Plasmodium falciparum using Bxb1 integrase and an intronic attB" @default.
- W2897296970 cites W1490272169 @default.
- W2897296970 cites W1801940882 @default.
- W2897296970 cites W1964575165 @default.
- W2897296970 cites W1981118697 @default.
- W2897296970 cites W1995045284 @default.
- W2897296970 cites W2004803790 @default.
- W2897296970 cites W2009106952 @default.
- W2897296970 cites W2011896954 @default.
- W2897296970 cites W2016728754 @default.
- W2897296970 cites W2020806892 @default.
- W2897296970 cites W2023408468 @default.
- W2897296970 cites W2023545713 @default.
- W2897296970 cites W2023981583 @default.
- W2897296970 cites W2032078800 @default.
- W2897296970 cites W2049441992 @default.
- W2897296970 cites W2055730063 @default.
- W2897296970 cites W2058302605 @default.
- W2897296970 cites W2066373250 @default.
- W2897296970 cites W2068613608 @default.
- W2897296970 cites W2074543807 @default.
- W2897296970 cites W2077300851 @default.
- W2897296970 cites W2077597524 @default.
- W2897296970 cites W2081761621 @default.
- W2897296970 cites W2082680042 @default.
- W2897296970 cites W2088626970 @default.
- W2897296970 cites W2098620195 @default.
- W2897296970 cites W2100039127 @default.
- W2897296970 cites W2106198832 @default.
- W2897296970 cites W2106959091 @default.
- W2897296970 cites W2109937218 @default.
- W2897296970 cites W2118737272 @default.
- W2897296970 cites W2123427466 @default.
- W2897296970 cites W2138199665 @default.
- W2897296970 cites W2145741131 @default.
- W2897296970 cites W2155471509 @default.
- W2897296970 cites W2158377245 @default.
- W2897296970 cites W2238012416 @default.
- W2897296970 cites W2252568502 @default.
- W2897296970 cites W2281388501 @default.
- W2897296970 cites W2320132985 @default.
- W2897296970 cites W2397788564 @default.
- W2897296970 cites W2515552791 @default.
- W2897296970 cites W2590802442 @default.
- W2897296970 cites W2596280611 @default.
- W2897296970 cites W2715361283 @default.
- W2897296970 cites W2726053863 @default.
- W2897296970 cites W2726777421 @default.
- W2897296970 cites W2765581562 @default.
- W2897296970 cites W2802404487 @default.
- W2897296970 cites W2808484967 @default.
- W2897296970 cites W2883697525 @default.
- W2897296970 cites W3021924366 @default.
- W2897296970 cites W4233982906 @default.
- W2897296970 doi "https://doi.org/10.1186/s13071-018-3129-5" @default.
- W2897296970 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6192176" @default.
- W2897296970 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30333047" @default.
- W2897296970 hasPublicationYear "2018" @default.
- W2897296970 type Work @default.
- W2897296970 sameAs 2897296970 @default.
- W2897296970 citedByCount "1" @default.
- W2897296970 countsByYear W28972969702022 @default.
- W2897296970 crossrefType "journal-article" @default.
- W2897296970 hasAuthorship W2897296970A5046001598 @default.
- W2897296970 hasAuthorship W2897296970A5085765149 @default.
- W2897296970 hasBestOaLocation W28972969701 @default.
- W2897296970 hasConcept C104317684 @default.
- W2897296970 hasConcept C132455925 @default.
- W2897296970 hasConcept C141231307 @default.
- W2897296970 hasConcept C14430832 @default.
- W2897296970 hasConcept C144501496 @default.
- W2897296970 hasConcept C203014093 @default.
- W2897296970 hasConcept C2778048844 @default.
- W2897296970 hasConcept C2778371730 @default.
- W2897296970 hasConcept C54355233 @default.
- W2897296970 hasConcept C70721500 @default.
- W2897296970 hasConcept C86803240 @default.
- W2897296970 hasConcept C98108389 @default.
- W2897296970 hasConceptScore W2897296970C104317684 @default.
- W2897296970 hasConceptScore W2897296970C132455925 @default.
- W2897296970 hasConceptScore W2897296970C141231307 @default.
- W2897296970 hasConceptScore W2897296970C14430832 @default.
- W2897296970 hasConceptScore W2897296970C144501496 @default.
- W2897296970 hasConceptScore W2897296970C203014093 @default.
- W2897296970 hasConceptScore W2897296970C2778048844 @default.
- W2897296970 hasConceptScore W2897296970C2778371730 @default.
- W2897296970 hasConceptScore W2897296970C54355233 @default.
- W2897296970 hasConceptScore W2897296970C70721500 @default.
- W2897296970 hasConceptScore W2897296970C86803240 @default.
- W2897296970 hasConceptScore W2897296970C98108389 @default.
- W2897296970 hasFunder F4320337355 @default.
- W2897296970 hasLocation W28972969701 @default.
- W2897296970 hasLocation W28972969702 @default.
- W2897296970 hasLocation W28972969703 @default.