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- W2897310731 abstract "The alcohol preference model is one of the most widely used animal models relevant to alcoholism. Stressors increase alcohol consumption. Here we present a protocol for a rapid and useful tool to test alcohol preference and stress-induced alcohol consumption in mice. In this model, animals are given two bottles, one with a diluted solution of ethanol in water, and the other with tap water. Consumption from each bottle is monitored over a 24-h period over several days to assess the animal's relative preference for the ethanol solution over water. In the second phase, animals are stressed by restraining them for an hour daily and their subsequent preference of tap water or the ethanol solution is evaluated. Preference is measured by the volume and/or weight or liquid consumed daily, which is then converted to a preference ratio. The alcohol preference model was combined with the conditioned place preference paradigm to determine alcohol conditioning and preference following the deletion of CB2 cannabinoid receptors in dopaminergic neurons in the DAT-Cnr2 Cre-recombinant conditional knockout (cKO) mice in comparison with the wild-type control mice." @default.
- W2897310731 created "2018-10-26" @default.
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- W2897310731 date "2018-01-01" @default.
- W2897310731 modified "2023-09-27" @default.
- W2897310731 title "Behavioral Evaluation of Seeking and Preference of Alcohol in Mice Subjected to Stress" @default.
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- W2897310731 doi "https://doi.org/10.21769/bioprotoc.3061" @default.
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