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• W2897316557 endingPage "961" @default.
• W2897316557 startingPage "949" @default.
• W2897316557 abstract "ABSTRACT Background : Cobra bite is frequently reported across the Indian subcontinent and is associated with a high rate of death and morbidity. In eastern India (EI) Naja naja and Naja kaouthia are reported to be the two most abundant species of cobra. Research design and methods : The venom proteome composition of N. naja (NnV) and N. kaouthia (NkV) from Burdwan districts of EI were compared by separation of venom proteins by 1D-SDS-PAGE followed by LC-MS/MS analysis of protein bands. The potency of commercial polyantivenom (PAV) was assessed by neutralization, ELISA, immuno-blot and venom-PAV immunoaffinity chromatography studies. Results : Proteomic analysis identified 52 and 55 proteins for NnV and NkV, respectively, when searched against the Elapidae database. A small quantitative difference in venom composition between these two species of cobra was observed. PAVs exhibited poor cross-reactivity against low molecular mass toxins (<20 kDa) of both cobra venoms, which was substantiated by a meager neutralization of their phospholipase A2 activity. Phospholipase A2 and 3FTx, the two major classes of nonenzymatic and enzymatic proteins, respectively, were partially recognized by PAVs. Conclusions : Efforts must be made to improve immunization protocols and supplement existing antivenoms with antibodies raised against the major toxins of these venoms." @default.
• W2897316557 created "2018-10-26" @default.
• W2897316557 creator A5032118191 @default.
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• W2897316557 creator A5061825257 @default.
• W2897316557 creator A5062474704 @default.
• W2897316557 date "2018-10-31" @default.
• W2897316557 modified "2023-10-15" @default.
• W2897316557 title "Proteomics analysis to compare the venom composition between <i>Naja naja</i> and <i>Naja kaouthia</i> from the same geographical location of eastern India: Correlation with pathophysiology of envenomation and immunological cross-reactivity towards commercial polyantivenom" @default.
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• W2897316557 doi "https://doi.org/10.1080/14789450.2018.1538799" @default.
• W2897316557 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30345852" @default.
• W2897316557 hasPublicationYear "2018" @default.
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