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- W2897331326 abstract "<ns4:p><ns4:bold>Background:</ns4:bold>The aim of this study was to analyze the synergistic relationship between<ns4:italic>Candida albicans</ns4:italic>and<ns4:italic>Streptococcus mutans</ns4:italic>in children with early childhood caries (ECC) experience.</ns4:p><ns4:p><ns4:bold>Methods:</ns4:bold>Dental plaque and unstimulated saliva samples were taken from 30 subjects aged 3-5 years old, half with (n=15, dmft > 4) and half without (n=15) ECC. The abundance of<ns4:italic>C. albicans</ns4:italic>and<ns4:italic>S. mutans</ns4:italic>and relative to total bacteria load were quantify by real-time PCR (qPCR). This method was also employed to investigate the mRNA expression of glycosyltransferase (<ns4:italic>gtfB</ns4:italic>) gene in dental plaque. Student’s t-test and Pearson’s correlation were used to perform statistical analysis.</ns4:p><ns4:p><ns4:bold>Results:</ns4:bold>Within the ECC group, the quantity of both microorganisms were higher in the saliva than in dental plaque. The ratio of<ns4:italic>C. albicans</ns4:italic>to total bacteria was higher in saliva than in plaque samples (p < 0.05). We observed the opposite for<ns4:italic>S. mutans</ns4:italic>(p < 0.05). The different value of<ns4:italic>C. albicans</ns4:italic>and<ns4:italic>S. mutans</ns4:italic>in saliva was positively correlated, and negatively correlated in dental plaque. Transcription level of<ns4:italic>S. mutans gtfB</ns4:italic>showed a positive correlation with<ns4:italic>C. albicans</ns4:italic>concentration in dental plaque. </ns4:p><ns4:p><ns4:bold>Conclusion:</ns4:bold><ns4:italic>C. albicans</ns4:italic>has a positive correlation with cariogenic traits of<ns4:italic>S. mutans</ns4:italic>in ECC-related biofilm of young children.</ns4:p>" @default.
- W2897331326 created "2018-10-26" @default.
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- W2897331326 date "2018-12-06" @default.
- W2897331326 modified "2023-09-27" @default.
- W2897331326 title "Relationship between Candida albicans and Streptococcus mutans in early childhood caries, evaluated by quantitative PCR" @default.
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- W2897331326 doi "https://doi.org/10.12688/f1000research.16275.2" @default.
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