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• W2897375868 endingPage "13447" @default.
• W2897375868 startingPage "13438" @default.
• W2897375868 abstract "Syngas fermentation to ethanol and acetate has recently been coupled to microbial chain elongation to produce medium-chain carboxylates, including n-caproate and n-caprylate. These medium-chain carboxylates are relatively hydrophobic, and thus easier to extract from solution than miscible ethanol. Here, we examined the effect of 11 different ethanol-to-acetate substrate ratios (ranging from 1.8 to 275 g COD g COD-1 [1.2 to 183 mol mol-1]) on directing chain elongation toward n-caprylate in a 0.7-L upflow anaerobic filter with product extraction. During an eight-month operating period, we monitored the performance and characterized the microbiome composition of this chain-elongating bioreactor. We also developed a thermodynamic model to predict the favorability of n-caprylate production at different substrate ratios. As predicted by our model, higher ethanol-to-acetate substrate ratios fed to our bioreactor led to higher specificities for n-caprylate production. We observed that feeding primarily ethanol to the bioreactor (i.e., ethanol-to-acetate substrate ratio of 275 g COD g COD-1) resulted in the highest specificity for n-caprylate, but the n-caprylate production rate decreased at this high ratio, resulting in lower conversion efficiencies. Thus, care should be taken not to overload the system with primarily ethanol as the substrate and to lower the organic loading rate." @default.
• W2897375868 created "2018-10-26" @default.
• W2897375868 creator A5006134961 @default.
• W2897375868 creator A5023885562 @default.
• W2897375868 creator A5038980069 @default.
• W2897375868 date "2018-10-18" @default.
• W2897375868 modified "2023-10-17" @default.
• W2897375868 title "Higher Substrate Ratios of Ethanol to Acetate Steered Chain Elongation toward <i>n</i>-Caprylate in a Bioreactor with Product Extraction" @default.
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• W2897375868 doi "https://doi.org/10.1021/acs.est.8b03856" @default.
• W2897375868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30335369" @default.
• W2897375868 hasPublicationYear "2018" @default.
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