Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897388749> ?p ?o ?g. }
- W2897388749 abstract "Abstract Solanum trilobatum L. is an important medicinal plant in traditional Indian system of medicine belonging to Solanaceae family. However, non-availability of genomic resources hinders its research at the molecular level. We have analyzed the S. trilobatum leaf transcriptome using high throughput RNA sequencing. The de novo assembly of 136,220,612 reads produced 128,934 non-redundant unigenes with N50 value of 1347 bp. Annotation of unigenes was performed against databases such as NCBI nr database, Gene Ontology, KEGG, Uniprot, Pfam, and plnTFDB. A total of 60,097 unigenes were annotated including 48 Transcription Factor families and 14,490 unigenes were assigned to 138 pathways using KEGG database. The pathway analysis revealed the transcripts involved in the biosynthesis of important secondary metabolites contributing for its medicinal value such as Flavonoids. Further, the transcripts were quantified using RSEM to identify the highly regulated genes for secondary metabolism. Reverse-Transcription PCR was performed to validate the de novo assembled unigenes. The expression profile of selected unigenes from flavonoid biosynthesis pathway was analyzed using qRT-PCR. We have also identified 13,262 Simple Sequence Repeats, which could help in molecular breeding. This is the first report of comprehensive transcriptome analysis in S. trilobatum and this will be an invaluable resource to understand the molecular basis related to the medicinal attributes of S. trilobatum in further studies." @default.
- W2897388749 created "2018-10-26" @default.
- W2897388749 creator A5004924582 @default.
- W2897388749 creator A5015825502 @default.
- W2897388749 creator A5018838714 @default.
- W2897388749 date "2018-10-18" @default.
- W2897388749 modified "2023-10-12" @default.
- W2897388749 title "RNA sequencing and de novo assembly of Solanum trilobatum leaf transcriptome to identify putative transcripts for major metabolic pathways" @default.
- W2897388749 cites W1567167300 @default.
- W2897388749 cites W1568479515 @default.
- W2897388749 cites W1809721560 @default.
- W2897388749 cites W1942083795 @default.
- W2897388749 cites W1963603642 @default.
- W2897388749 cites W1966542159 @default.
- W2897388749 cites W1970829860 @default.
- W2897388749 cites W1974116356 @default.
- W2897388749 cites W1995521793 @default.
- W2897388749 cites W1999134783 @default.
- W2897388749 cites W1999359847 @default.
- W2897388749 cites W1999574084 @default.
- W2897388749 cites W2001955647 @default.
- W2897388749 cites W2006516560 @default.
- W2897388749 cites W2006815797 @default.
- W2897388749 cites W2010982696 @default.
- W2897388749 cites W2028008904 @default.
- W2897388749 cites W2030594278 @default.
- W2897388749 cites W2036897871 @default.
- W2897388749 cites W2037438130 @default.
- W2897388749 cites W2041472962 @default.
- W2897388749 cites W2041679004 @default.
- W2897388749 cites W2046549863 @default.
- W2897388749 cites W2051520396 @default.
- W2897388749 cites W2051802812 @default.
- W2897388749 cites W2051974604 @default.
- W2897388749 cites W2055095632 @default.
- W2897388749 cites W2059364139 @default.
- W2897388749 cites W2062059117 @default.
- W2897388749 cites W2067026953 @default.
- W2897388749 cites W2071151647 @default.
- W2897388749 cites W2077322858 @default.
- W2897388749 cites W2081495127 @default.
- W2897388749 cites W2085134005 @default.
- W2897388749 cites W2092686336 @default.
- W2897388749 cites W2097190551 @default.
- W2897388749 cites W2107331309 @default.
- W2897388749 cites W2110256992 @default.
- W2897388749 cites W2126419817 @default.
- W2897388749 cites W2129013963 @default.
- W2897388749 cites W2137652007 @default.
- W2897388749 cites W2151404319 @default.
- W2897388749 cites W2156125289 @default.
- W2897388749 cites W2159482845 @default.
- W2897388749 cites W2161511344 @default.
- W2897388749 cites W2161802098 @default.
- W2897388749 cites W2164513408 @default.
- W2897388749 cites W2267134132 @default.
- W2897388749 cites W2559588208 @default.
- W2897388749 cites W2744370150 @default.
- W2897388749 cites W2745968749 @default.
- W2897388749 cites W4211254291 @default.
- W2897388749 cites W4294216483 @default.
- W2897388749 doi "https://doi.org/10.1038/s41598-018-33693-4" @default.
- W2897388749 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6194071" @default.
- W2897388749 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30337583" @default.
- W2897388749 hasPublicationYear "2018" @default.
- W2897388749 type Work @default.
- W2897388749 sameAs 2897388749 @default.
- W2897388749 citedByCount "17" @default.
- W2897388749 countsByYear W28973887492019 @default.
- W2897388749 countsByYear W28973887492020 @default.
- W2897388749 countsByYear W28973887492021 @default.
- W2897388749 countsByYear W28973887492022 @default.
- W2897388749 crossrefType "journal-article" @default.
- W2897388749 hasAuthorship W2897388749A5004924582 @default.
- W2897388749 hasAuthorship W2897388749A5015825502 @default.
- W2897388749 hasAuthorship W2897388749A5018838714 @default.
- W2897388749 hasBestOaLocation W28973887491 @default.
- W2897388749 hasConcept C104317684 @default.
- W2897388749 hasConcept C150194340 @default.
- W2897388749 hasConcept C152724338 @default.
- W2897388749 hasConcept C162317418 @default.
- W2897388749 hasConcept C18949551 @default.
- W2897388749 hasConcept C202264299 @default.
- W2897388749 hasConcept C2780004758 @default.
- W2897388749 hasConcept C50489715 @default.
- W2897388749 hasConcept C54355233 @default.
- W2897388749 hasConcept C70721500 @default.
- W2897388749 hasConcept C86803240 @default.
- W2897388749 hasConceptScore W2897388749C104317684 @default.
- W2897388749 hasConceptScore W2897388749C150194340 @default.
- W2897388749 hasConceptScore W2897388749C152724338 @default.
- W2897388749 hasConceptScore W2897388749C162317418 @default.
- W2897388749 hasConceptScore W2897388749C18949551 @default.
- W2897388749 hasConceptScore W2897388749C202264299 @default.
- W2897388749 hasConceptScore W2897388749C2780004758 @default.
- W2897388749 hasConceptScore W2897388749C50489715 @default.
- W2897388749 hasConceptScore W2897388749C54355233 @default.
- W2897388749 hasConceptScore W2897388749C70721500 @default.
- W2897388749 hasConceptScore W2897388749C86803240 @default.
- W2897388749 hasFunder F4320320717 @default.