Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897431175> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2897431175 abstract "Tissue clearing techniques are useful for large-scale three-dimensional fluorescence imaging of thick tissues. However, high-resolution imaging deep inside tissues has been challenging, as it is extremely sensitive to light scattering and spherical aberrations. Here, we present a water-based optical clearing and mounting media, SeeDB2, which is designed for high numerical aperture (NA) objective lenses with oil or glycerol immersion. Using quick and simple soaking procedures, the refractive indices of samples can be matched either to that of immersion oil (1.52) or glycerol (1.46), thus minimizing light scattering and spherical aberrations. Fine morphology and various fluorescent proteins are highly preserved during the clearing and imaging process. Our method is useful for the three-dimensional fluorescence imaging of neuronal circuitry at synaptic resolution using confocal and super-resolution microscopy. SeeDB2 is also useful as a mounting media for the super-resolution imaging of fluorescent proteins." @default.
- W2897431175 created "2018-10-26" @default.
- W2897431175 creator A5055258160 @default.
- W2897431175 creator A5081539043 @default.
- W2897431175 date "2018-01-01" @default.
- W2897431175 modified "2023-09-23" @default.
- W2897431175 title "Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2" @default.
- W2897431175 cites W1603300429 @default.
- W2897431175 cites W2078677652 @default.
- W2897431175 cites W2101545913 @default.
- W2897431175 cites W2296740725 @default.
- W2897431175 cites W2764268982 @default.
- W2897431175 doi "https://doi.org/10.21769/bioprotoc.3046" @default.
- W2897431175 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/8342116" @default.
- W2897431175 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34532520" @default.
- W2897431175 hasPublicationYear "2018" @default.
- W2897431175 type Work @default.
- W2897431175 sameAs 2897431175 @default.
- W2897431175 citedByCount "2" @default.
- W2897431175 countsByYear W28974311752018 @default.
- W2897431175 countsByYear W28974311752022 @default.
- W2897431175 crossrefType "journal-article" @default.
- W2897431175 hasAuthorship W2897431175A5055258160 @default.
- W2897431175 hasAuthorship W2897431175A5081539043 @default.
- W2897431175 hasBestOaLocation W28974311752 @default.
- W2897431175 hasConcept C113976765 @default.
- W2897431175 hasConcept C120456961 @default.
- W2897431175 hasConcept C120665830 @default.
- W2897431175 hasConcept C121332964 @default.
- W2897431175 hasConcept C136009344 @default.
- W2897431175 hasConcept C136229726 @default.
- W2897431175 hasConcept C138268822 @default.
- W2897431175 hasConcept C147080431 @default.
- W2897431175 hasConcept C154945302 @default.
- W2897431175 hasConcept C159108749 @default.
- W2897431175 hasConcept C169274487 @default.
- W2897431175 hasConcept C177634673 @default.
- W2897431175 hasConcept C191486275 @default.
- W2897431175 hasConcept C192562407 @default.
- W2897431175 hasConcept C41008148 @default.
- W2897431175 hasConcept C42067758 @default.
- W2897431175 hasConcept C49040817 @default.
- W2897431175 hasConcept C57477423 @default.
- W2897431175 hasConcept C6260449 @default.
- W2897431175 hasConcept C71924100 @default.
- W2897431175 hasConcept C91881484 @default.
- W2897431175 hasConceptScore W2897431175C113976765 @default.
- W2897431175 hasConceptScore W2897431175C120456961 @default.
- W2897431175 hasConceptScore W2897431175C120665830 @default.
- W2897431175 hasConceptScore W2897431175C121332964 @default.
- W2897431175 hasConceptScore W2897431175C136009344 @default.
- W2897431175 hasConceptScore W2897431175C136229726 @default.
- W2897431175 hasConceptScore W2897431175C138268822 @default.
- W2897431175 hasConceptScore W2897431175C147080431 @default.
- W2897431175 hasConceptScore W2897431175C154945302 @default.
- W2897431175 hasConceptScore W2897431175C159108749 @default.
- W2897431175 hasConceptScore W2897431175C169274487 @default.
- W2897431175 hasConceptScore W2897431175C177634673 @default.
- W2897431175 hasConceptScore W2897431175C191486275 @default.
- W2897431175 hasConceptScore W2897431175C192562407 @default.
- W2897431175 hasConceptScore W2897431175C41008148 @default.
- W2897431175 hasConceptScore W2897431175C42067758 @default.
- W2897431175 hasConceptScore W2897431175C49040817 @default.
- W2897431175 hasConceptScore W2897431175C57477423 @default.
- W2897431175 hasConceptScore W2897431175C6260449 @default.
- W2897431175 hasConceptScore W2897431175C71924100 @default.
- W2897431175 hasConceptScore W2897431175C91881484 @default.
- W2897431175 hasLocation W28974311751 @default.
- W2897431175 hasLocation W28974311752 @default.
- W2897431175 hasLocation W28974311753 @default.
- W2897431175 hasLocation W28974311754 @default.
- W2897431175 hasOpenAccess W2897431175 @default.
- W2897431175 hasPrimaryLocation W28974311751 @default.
- W2897431175 hasRelatedWork W1967446593 @default.
- W2897431175 hasRelatedWork W2025704708 @default.
- W2897431175 hasRelatedWork W2032690770 @default.
- W2897431175 hasRelatedWork W2036395878 @default.
- W2897431175 hasRelatedWork W2074388803 @default.
- W2897431175 hasRelatedWork W2099533084 @default.
- W2897431175 hasRelatedWork W2116113317 @default.
- W2897431175 hasRelatedWork W2126161242 @default.
- W2897431175 hasRelatedWork W2131302229 @default.
- W2897431175 hasRelatedWork W2140289797 @default.
- W2897431175 hasRelatedWork W2516333144 @default.
- W2897431175 hasRelatedWork W2755855010 @default.
- W2897431175 hasRelatedWork W2807523344 @default.
- W2897431175 hasRelatedWork W2902852116 @default.
- W2897431175 hasRelatedWork W2918398723 @default.
- W2897431175 hasRelatedWork W2991539402 @default.
- W2897431175 hasRelatedWork W3101172483 @default.
- W2897431175 hasRelatedWork W3184582020 @default.
- W2897431175 hasRelatedWork W2826138060 @default.
- W2897431175 hasRelatedWork W3121441745 @default.
- W2897431175 isParatext "false" @default.
- W2897431175 isRetracted "false" @default.
- W2897431175 magId "2897431175" @default.
- W2897431175 workType "article" @default.