Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897464952> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2897464952 abstract "Endocytosis is a fundamental process underlying diverse eukaryotic physiology. The terminal stage of this process is membrane fusion between the perimeter membrane of a late endosome filled with intraluminal vesicles, or multivesicular body (MVB), and the lysosome membrane to facilitate catabolism of internalized biomaterials or surface polytopic proteins. To comprehensively understand the mechanisms underlying MVB-lysosome membrane fusion, we developed a quantitative, cell-free assay to study this SNARE-mediated event in molecular detail using Saccharomyces cerevisiae and its vacuolar lysosome, or vacuole, as models. This involves separately isolating organelles from two yeast strains each expressing a different complementary fusion probe targeted to the lumen of either MVBs or vacuoles. Isolated organelles are mixed in vitro under fusogenic conditions. Upon MVB-vacuole membrane fusion, luminal contents mix to facilitate probe interaction, reconstituting β-lactamase activity recorded by a colorimetric enzyme activity assay. This method accommodates a multitude of approaches (e.g., genetics, addition of purified protein reagents) to study this process in isolation, and in theory could be repurposed to study other SNARE-mediated fusion events within cells." @default.
- W2897464952 created "2018-10-26" @default.
- W2897464952 creator A5057632375 @default.
- W2897464952 creator A5057761100 @default.
- W2897464952 creator A5091118370 @default.
- W2897464952 date "2018-10-14" @default.
- W2897464952 modified "2023-10-03" @default.
- W2897464952 title "A Cell-Free Content Mixing Assay for SNARE-Mediated Multivesicular Body-Vacuole Membrane Fusion" @default.
- W2897464952 cites W1893312779 @default.
- W2897464952 cites W1978698410 @default.
- W2897464952 cites W1982606786 @default.
- W2897464952 cites W2010766189 @default.
- W2897464952 cites W2011461336 @default.
- W2897464952 cites W2038565727 @default.
- W2897464952 cites W2069789629 @default.
- W2897464952 cites W2070709903 @default.
- W2897464952 cites W2070732930 @default.
- W2897464952 cites W2080143331 @default.
- W2897464952 cites W2105580296 @default.
- W2897464952 cites W2109691647 @default.
- W2897464952 cites W2110522042 @default.
- W2897464952 cites W2138537428 @default.
- W2897464952 cites W2170678054 @default.
- W2897464952 cites W2312836697 @default.
- W2897464952 cites W2596141601 @default.
- W2897464952 cites W2951754469 @default.
- W2897464952 cites W2953190444 @default.
- W2897464952 doi "https://doi.org/10.1007/978-1-4939-8760-3_19" @default.
- W2897464952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30317513" @default.
- W2897464952 hasPublicationYear "2018" @default.
- W2897464952 type Work @default.
- W2897464952 sameAs 2897464952 @default.
- W2897464952 citedByCount "2" @default.
- W2897464952 countsByYear W28974649522019 @default.
- W2897464952 countsByYear W28974649522021 @default.
- W2897464952 crossrefType "book-chapter" @default.
- W2897464952 hasAuthorship W2897464952A5057632375 @default.
- W2897464952 hasAuthorship W2897464952A5057761100 @default.
- W2897464952 hasAuthorship W2897464952A5091118370 @default.
- W2897464952 hasConcept C102568950 @default.
- W2897464952 hasConcept C102747710 @default.
- W2897464952 hasConcept C103038307 @default.
- W2897464952 hasConcept C130316041 @default.
- W2897464952 hasConcept C1491633281 @default.
- W2897464952 hasConcept C168240541 @default.
- W2897464952 hasConcept C181199279 @default.
- W2897464952 hasConcept C185592680 @default.
- W2897464952 hasConcept C190062978 @default.
- W2897464952 hasConcept C2777576037 @default.
- W2897464952 hasConcept C2779222958 @default.
- W2897464952 hasConcept C2780216420 @default.
- W2897464952 hasConcept C28005876 @default.
- W2897464952 hasConcept C41625074 @default.
- W2897464952 hasConcept C55493867 @default.
- W2897464952 hasConcept C79879829 @default.
- W2897464952 hasConcept C86803240 @default.
- W2897464952 hasConcept C95444343 @default.
- W2897464952 hasConceptScore W2897464952C102568950 @default.
- W2897464952 hasConceptScore W2897464952C102747710 @default.
- W2897464952 hasConceptScore W2897464952C103038307 @default.
- W2897464952 hasConceptScore W2897464952C130316041 @default.
- W2897464952 hasConceptScore W2897464952C1491633281 @default.
- W2897464952 hasConceptScore W2897464952C168240541 @default.
- W2897464952 hasConceptScore W2897464952C181199279 @default.
- W2897464952 hasConceptScore W2897464952C185592680 @default.
- W2897464952 hasConceptScore W2897464952C190062978 @default.
- W2897464952 hasConceptScore W2897464952C2777576037 @default.
- W2897464952 hasConceptScore W2897464952C2779222958 @default.
- W2897464952 hasConceptScore W2897464952C2780216420 @default.
- W2897464952 hasConceptScore W2897464952C28005876 @default.
- W2897464952 hasConceptScore W2897464952C41625074 @default.
- W2897464952 hasConceptScore W2897464952C55493867 @default.
- W2897464952 hasConceptScore W2897464952C79879829 @default.
- W2897464952 hasConceptScore W2897464952C86803240 @default.
- W2897464952 hasConceptScore W2897464952C95444343 @default.
- W2897464952 hasLocation W28974649521 @default.
- W2897464952 hasLocation W28974649522 @default.
- W2897464952 hasOpenAccess W2897464952 @default.
- W2897464952 hasPrimaryLocation W28974649521 @default.
- W2897464952 hasRelatedWork W1953178180 @default.
- W2897464952 hasRelatedWork W195380496 @default.
- W2897464952 hasRelatedWork W2037732687 @default.
- W2897464952 hasRelatedWork W2071631353 @default.
- W2897464952 hasRelatedWork W2090502005 @default.
- W2897464952 hasRelatedWork W2097631517 @default.
- W2897464952 hasRelatedWork W2110645858 @default.
- W2897464952 hasRelatedWork W2739564059 @default.
- W2897464952 hasRelatedWork W2951754469 @default.
- W2897464952 hasRelatedWork W3046223369 @default.
- W2897464952 isParatext "false" @default.
- W2897464952 isRetracted "false" @default.
- W2897464952 magId "2897464952" @default.
- W2897464952 workType "book-chapter" @default.