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• W2897523069 abstract "Extracellular matrix‐based hydrogels such as Matrigel are easy‐to‐use, commercially available, and offer environments for three‐dimensional (3‐D) cell culture that mimic native tissue. However, manipulating small volumes of these materials to produce thin‐layer 3‐D culture systems suitable for analysis is difficult because of air–liquid‐substrate interfacial tension effects and evaporation. Here, we demonstrate two simple techniques that use standard liquid‐handling tools and nontreated 96‐well plates to produce uniform, thin‐layer constructs for 3‐D culture of cells in Matrigel. The first technique, the floating 3‐D cell culture method, uses phase‐separating polymers to form a barrier between the dispensed Matrigel, air, and cultureware surface to generate consistently thin hydrogels from volumes as low as 5 μL. These unanchored gels provide a useful assay for investigating airway smooth muscle cell contraction and may have future applications in studying asthma pathophysiology. The second technique, the fixed 3‐D cell culture method, provides an anchored gel system for culturing noncontractile cells (e.g., neurons) where 20 μL of Matrigel is dispensed into the bottom of a well filled with culture medium to form a thin gel containing embedded cells. This technique has potential widespread applications as an accessible 3‐D culture platform for high‐throughput production of disease models for evaluation of novel drug therapies. © 2018 American Institute of Chemical Engineers Biotechnol. Prog ., 35: e2733, 2019" @default.
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• W2897523069 date "2018-11-17" @default.
• W2897523069 modified "2023-09-27" @default.
• W2897523069 title "Fabrication of thin-layer matrigel-based constructs for three-dimensional cell culture" @default.
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• W2897523069 doi "https://doi.org/10.1002/btpr.2733" @default.
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