Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897530386> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2897530386 abstract "Several skin disorders, like chronic wounds and psoriasis involve several steps for skin regeneration. On woundrepair keratinocyte migration for reepithelialization is required. Psoriatic lesions result from the interactionbetween hyperproliferative keratinocytes, inflammatory cells and immune cells. Chicken feather (CF) arecomposed by keratin and its disposal is a critical problem. It is possible to hydrolyze (CF) to obtain high valuekeratin peptides minimizing the impact on the environment and giving value to otherwise waste material.Keratin peptides (KP) were obtained by submerged fermentation using a Bacillus cereus strain (S188D) growingon keratin. Briefly, 1 g of CF was incubated with 100 mL of growing media at 30oC and 200 rpm for 48h. The KPwere subjected to different purification procedures: i) isolated using a C18 column (stage1); ii) eluted using a cut-off of 5kDA and fractioned with different percentages of acetonitrile 20% and 40% (stage2); iii) each fraction waseluted using a Superdex peptide column to obtain different chromatographic picks, P1, P2, P3, P4, and P5(stage3). The keratinocytes cells were grown in DMEM supplement with 10% of FBS at 37oC and 5% of CO2.Cells were grown till confluence and a scratch was performed; 45 mg/mL of the KP, from the 3 stages ofpurification were placed in contact with the cells for 24h. When the stage1 KP were in contact with the cells therewas a decrease on cell migration in comparison to the control. The second stage peptides (20% and 40%) did notinduce any significant changes on the cell migration, but the presence of the peptides significantly decrease cellproliferation in 43% and 54%, respectively. An opposite result was observed for the third stage peptides, S188D20% P1, P2, P3 and S188D 40% P3, seems to increase cell migration, while S188D P4 and P5 significantlyincreased cell migration in 20% and 19%, respectively. Only S188D 40% P2 significantly increased cellproliferation. Depending on the fraction of KP it is possible to obtain opposite effects regarding cell migration. Itis possible to modulate the cellular response of the keratinocytes, by using simple fractioning. The KP used onthis study were sequenced and the mechanisms that behind these results are being evaluated" @default.
- W2897530386 created "2018-10-26" @default.
- W2897530386 creator A5006853324 @default.
- W2897530386 creator A5016349563 @default.
- W2897530386 creator A5047637727 @default.
- W2897530386 creator A5059153825 @default.
- W2897530386 creator A5076379209 @default.
- W2897530386 date "2018-09-09" @default.
- W2897530386 modified "2023-09-26" @default.
- W2897530386 title "Keratin bioactive peptides for the treatment of skin disorders" @default.
- W2897530386 hasPublicationYear "2018" @default.
- W2897530386 type Work @default.
- W2897530386 sameAs 2897530386 @default.
- W2897530386 citedByCount "0" @default.
- W2897530386 crossrefType "journal-article" @default.
- W2897530386 hasAuthorship W2897530386A5006853324 @default.
- W2897530386 hasAuthorship W2897530386A5016349563 @default.
- W2897530386 hasAuthorship W2897530386A5047637727 @default.
- W2897530386 hasAuthorship W2897530386A5059153825 @default.
- W2897530386 hasAuthorship W2897530386A5076379209 @default.
- W2897530386 hasConcept C105702510 @default.
- W2897530386 hasConcept C127745971 @default.
- W2897530386 hasConcept C142724271 @default.
- W2897530386 hasConcept C185592680 @default.
- W2897530386 hasConcept C18903297 @default.
- W2897530386 hasConcept C202751555 @default.
- W2897530386 hasConcept C203014093 @default.
- W2897530386 hasConcept C2776458125 @default.
- W2897530386 hasConcept C2777074287 @default.
- W2897530386 hasConcept C2780269544 @default.
- W2897530386 hasConcept C43617362 @default.
- W2897530386 hasConcept C55493867 @default.
- W2897530386 hasConcept C71924100 @default.
- W2897530386 hasConcept C73826308 @default.
- W2897530386 hasConcept C81241287 @default.
- W2897530386 hasConcept C86803240 @default.
- W2897530386 hasConceptScore W2897530386C105702510 @default.
- W2897530386 hasConceptScore W2897530386C127745971 @default.
- W2897530386 hasConceptScore W2897530386C142724271 @default.
- W2897530386 hasConceptScore W2897530386C185592680 @default.
- W2897530386 hasConceptScore W2897530386C18903297 @default.
- W2897530386 hasConceptScore W2897530386C202751555 @default.
- W2897530386 hasConceptScore W2897530386C203014093 @default.
- W2897530386 hasConceptScore W2897530386C2776458125 @default.
- W2897530386 hasConceptScore W2897530386C2777074287 @default.
- W2897530386 hasConceptScore W2897530386C2780269544 @default.
- W2897530386 hasConceptScore W2897530386C43617362 @default.
- W2897530386 hasConceptScore W2897530386C55493867 @default.
- W2897530386 hasConceptScore W2897530386C71924100 @default.
- W2897530386 hasConceptScore W2897530386C73826308 @default.
- W2897530386 hasConceptScore W2897530386C81241287 @default.
- W2897530386 hasConceptScore W2897530386C86803240 @default.
- W2897530386 hasLocation W28975303861 @default.
- W2897530386 hasOpenAccess W2897530386 @default.
- W2897530386 hasPrimaryLocation W28975303861 @default.
- W2897530386 hasRelatedWork W1501508768 @default.
- W2897530386 hasRelatedWork W152211590 @default.
- W2897530386 hasRelatedWork W16984528 @default.
- W2897530386 hasRelatedWork W1966552558 @default.
- W2897530386 hasRelatedWork W1977540748 @default.
- W2897530386 hasRelatedWork W1980657293 @default.
- W2897530386 hasRelatedWork W1990308825 @default.
- W2897530386 hasRelatedWork W1997990964 @default.
- W2897530386 hasRelatedWork W2015662540 @default.
- W2897530386 hasRelatedWork W2021854922 @default.
- W2897530386 hasRelatedWork W2036425785 @default.
- W2897530386 hasRelatedWork W2063396119 @default.
- W2897530386 hasRelatedWork W2068496256 @default.
- W2897530386 hasRelatedWork W2072117102 @default.
- W2897530386 hasRelatedWork W2092416679 @default.
- W2897530386 hasRelatedWork W2304535303 @default.
- W2897530386 hasRelatedWork W253512704 @default.
- W2897530386 hasRelatedWork W3030248720 @default.
- W2897530386 hasRelatedWork W3152412552 @default.
- W2897530386 hasRelatedWork W603578719 @default.
- W2897530386 isParatext "false" @default.
- W2897530386 isRetracted "false" @default.
- W2897530386 magId "2897530386" @default.
- W2897530386 workType "article" @default.