Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897536134> ?p ?o ?g. }
- W2897536134 abstract "MOK, a pharmacopuncture medicine consisting of 10 herbs, has a long history as treatment for various inflammatory conditions. To investigate the mechanisms of action of MOK, its anti‑inflammatory and antioxidative effects were assessed in RAW 264.7 macrophages stimulated by lipopolysaccharide (LPS). RAW 264.7 cells were treated with different concentrations of MOK extract for 30 min prior to stimulation with or without LPS for the indicated times. Nitric oxide (NO) production was measured using Griess reagent, while the mRNA levels of inflammatory cytokines, tumor necrosis factor (TNF)‑α, interleukin (IL)‑1β, IL‑6 and the antioxidant enzymes Mn superoxide dismutase and heme oxygenase‑1, were determined using reverse transcription‑polymerase chain reaction analysis. Western blotting was used to determine the protein expression of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)‑2, superoxide dismutase (SOD)2, catalase (CAT) and heme oxygenase‑1 (HO‑1), and the phosphorylation of mitogen‑activated protein kinases (MAPKs), including ERK1/2, JNK and p38. Western blotting and immunocytochemistry were used to observe the nuclear expression of nuclear factor (NF)‑κB p65. Additionally, reactive oxygen species (ROS) and prostaglandin (PG)E2 production were determined using the ROS assay and an enzyme immunoassay. With MOK treatment, there was a notable decrease in NO and PGE2 production induced by LPS in RAW 264.7 cells by downregulation of iNOS and COX‑2 mRNA and protein expression. Furthermore, with MOK treatment, there was a decrease in the mRNA expression levels of TNF‑α, IL‑1β and IL‑6, as well as in the phosphorylation of ERK, JNK and p38 MAPK, by blocking the nuclear translocation of NF‑κB p65 in LPS‑stimulated cells. In addition, MOK treatment led to an increase in the antioxidant enzymes SOD, CAT and HO‑1 in LPS‑stimulated cells, with a concomitant decrease in ROS generation. These results indicate that the inflammatory responses in activated macrophages are inhibited by MOK through downregulation of the transcription levels of inflammatory mediators and inhibition of the MAPK/NF‑κB pathway. Moreover, MOK protects against oxidative damage by upregulating the expression of antioxidant enzymes and generating ROS scavengers." @default.
- W2897536134 created "2018-10-26" @default.
- W2897536134 creator A5018228594 @default.
- W2897536134 creator A5034098399 @default.
- W2897536134 creator A5056953271 @default.
- W2897536134 creator A5063908813 @default.
- W2897536134 creator A5088688800 @default.
- W2897536134 date "2018-10-16" @default.
- W2897536134 modified "2023-09-25" @default.
- W2897536134 title "Anti-inflammatory and antioxidant effects of MOK, a polyherbal extract, on lipopolysaccharide‑stimulated RAW 264.7 macrophages" @default.
- W2897536134 cites W1614231230 @default.
- W2897536134 cites W1822061653 @default.
- W2897536134 cites W1952301860 @default.
- W2897536134 cites W1956303539 @default.
- W2897536134 cites W1968876514 @default.
- W2897536134 cites W1976933274 @default.
- W2897536134 cites W1995039600 @default.
- W2897536134 cites W2001224007 @default.
- W2897536134 cites W2012992792 @default.
- W2897536134 cites W2014106971 @default.
- W2897536134 cites W2016281184 @default.
- W2897536134 cites W2018607958 @default.
- W2897536134 cites W2029061945 @default.
- W2897536134 cites W2031100732 @default.
- W2897536134 cites W2032093603 @default.
- W2897536134 cites W2049450575 @default.
- W2897536134 cites W2056098567 @default.
- W2897536134 cites W2059983531 @default.
- W2897536134 cites W2061177902 @default.
- W2897536134 cites W2066607937 @default.
- W2897536134 cites W2068392219 @default.
- W2897536134 cites W2071695743 @default.
- W2897536134 cites W2071746830 @default.
- W2897536134 cites W2079089102 @default.
- W2897536134 cites W2084141237 @default.
- W2897536134 cites W2091535118 @default.
- W2897536134 cites W2107946946 @default.
- W2897536134 cites W2113394933 @default.
- W2897536134 cites W2118989452 @default.
- W2897536134 cites W2119394260 @default.
- W2897536134 cites W2141635648 @default.
- W2897536134 cites W2171440607 @default.
- W2897536134 cites W2292383634 @default.
- W2897536134 cites W2335535048 @default.
- W2897536134 cites W2567832150 @default.
- W2897536134 cites W2771821060 @default.
- W2897536134 cites W2795346947 @default.
- W2897536134 cites W2803280239 @default.
- W2897536134 cites W4234375654 @default.
- W2897536134 doi "https://doi.org/10.3892/ijmm.2018.3937" @default.
- W2897536134 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6257867" @default.
- W2897536134 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30365058" @default.
- W2897536134 hasPublicationYear "2018" @default.
- W2897536134 type Work @default.
- W2897536134 sameAs 2897536134 @default.
- W2897536134 citedByCount "17" @default.
- W2897536134 countsByYear W28975361342020 @default.
- W2897536134 countsByYear W28975361342021 @default.
- W2897536134 countsByYear W28975361342022 @default.
- W2897536134 countsByYear W28975361342023 @default.
- W2897536134 crossrefType "journal-article" @default.
- W2897536134 hasAuthorship W2897536134A5018228594 @default.
- W2897536134 hasAuthorship W2897536134A5034098399 @default.
- W2897536134 hasAuthorship W2897536134A5056953271 @default.
- W2897536134 hasAuthorship W2897536134A5063908813 @default.
- W2897536134 hasAuthorship W2897536134A5088688800 @default.
- W2897536134 hasBestOaLocation W28975361341 @default.
- W2897536134 hasConcept C104317684 @default.
- W2897536134 hasConcept C153911025 @default.
- W2897536134 hasConcept C178790620 @default.
- W2897536134 hasConcept C17991360 @default.
- W2897536134 hasConcept C181199279 @default.
- W2897536134 hasConcept C185592680 @default.
- W2897536134 hasConcept C203014093 @default.
- W2897536134 hasConcept C2775838275 @default.
- W2897536134 hasConcept C2776151105 @default.
- W2897536134 hasConcept C2776217839 @default.
- W2897536134 hasConcept C2777622882 @default.
- W2897536134 hasConcept C2778754761 @default.
- W2897536134 hasConcept C2779219270 @default.
- W2897536134 hasConcept C48349386 @default.
- W2897536134 hasConcept C519581460 @default.
- W2897536134 hasConcept C55493867 @default.
- W2897536134 hasConcept C85265743 @default.
- W2897536134 hasConcept C86803240 @default.
- W2897536134 hasConceptScore W2897536134C104317684 @default.
- W2897536134 hasConceptScore W2897536134C153911025 @default.
- W2897536134 hasConceptScore W2897536134C178790620 @default.
- W2897536134 hasConceptScore W2897536134C17991360 @default.
- W2897536134 hasConceptScore W2897536134C181199279 @default.
- W2897536134 hasConceptScore W2897536134C185592680 @default.
- W2897536134 hasConceptScore W2897536134C203014093 @default.
- W2897536134 hasConceptScore W2897536134C2775838275 @default.
- W2897536134 hasConceptScore W2897536134C2776151105 @default.
- W2897536134 hasConceptScore W2897536134C2776217839 @default.
- W2897536134 hasConceptScore W2897536134C2777622882 @default.
- W2897536134 hasConceptScore W2897536134C2778754761 @default.
- W2897536134 hasConceptScore W2897536134C2779219270 @default.
- W2897536134 hasConceptScore W2897536134C48349386 @default.
- W2897536134 hasConceptScore W2897536134C519581460 @default.