FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897545321> ?p ?o ?g. }

• W2897545321 endingPage "3190" @default.
• W2897545321 startingPage "3173" @default.
• W2897545321 abstract "Inflammatory gene expression is modulated by posttranscriptional regulation via RNA-binding proteins (RBPs), which regulate mRNA turnover and translation by binding to conserved mRNA sequences. Their role in COPD is only partially defined. This study evaluated RBPs tristetraprolin (TTP), human antigen R (HuR), and AU-rich element-binding factor 1 (AUF-1) expression using lung tissue from COPD patients and control subjects and probed their function in epithelial responses in vitro.RBPs were detected by immunohistochemistry in bronchial and peripheral lung samples from mild-to-moderate stable COPD patients and age/smoking history-matched controls; RBPs and RBP-regulated genes were evaluated by Western blot, ELISA, protein array, and real-time PCR in human airway epithelial BEAS-2B cell line stimulated with hydrogen peroxide, cytokine combination (cytomix), cigarette smoke extract (CSE), and following siRNA-mediated silencing. Results were verified in a microarray database from bronchial brushings of COPD patients and controls. RBP transcripts were measured in peripheral blood mononuclear cell samples from additional stable COPD patients and controls.Specific, primarily nuclear immunostaining for the RBPs was detected in structural and inflammatory cells in bronchial and lung tissues. Immunostaining for AUF-1, but not TTP or HuR, was significantly decreased in bronchial epithelium of COPD samples vs controls. In BEAS-2B cells, cytomix and CSE stimulation reproduced the RBP pattern while increasing expression of AUF-1-regulated genes, interleukin-6, CCL2, CXCL1, and CXCL8. Silencing expression of AUF-1 reproduced, but not enhanced, target upregulation induced by cytomix compared to controls. Analysis of bronchial brushing-derived transcriptomic confirmed the selective decrease of AUF-1 in COPD vs controls and revealed significant changes in AUF-1-regulated genes by genome ontology.Downregulated AUF-1 may be pathogenic in stable COPD by altering posttranscriptional control of epithelial gene expression." @default.
• W2897545321 created "2018-10-26" @default.
• W2897545321 creator A5011393194 @default.
• W2897545321 creator A5013475705 @default.
• W2897545321 creator A5016161340 @default.
• W2897545321 creator A5024578403 @default.
• W2897545321 creator A5024644508 @default.
• W2897545321 creator A5055881015 @default.
• W2897545321 creator A5067185484 @default.
• W2897545321 creator A5071152137 @default.
• W2897545321 creator A5073265989 @default.
• W2897545321 creator A5080718228 @default.
• W2897545321 date "2018-10-01" @default.
• W2897545321 modified "2023-09-29" @default.
• W2897545321 title "Differential expression of RNA-binding proteins in bronchial epithelium of stable COPD patients" @default.
• W2897545321 cites W1560505485 @default.
• W2897545321 cites W1763340921 @default.
• W2897545321 cites W1884758446 @default.
• W2897545321 cites W1963482227 @default.
• W2897545321 cites W1973070843 @default.
• W2897545321 cites W1985002063 @default.
• W2897545321 cites W1986147729 @default.
• W2897545321 cites W1986521568 @default.
• W2897545321 cites W1997272400 @default.
• W2897545321 cites W1997535872 @default.
• W2897545321 cites W1997773608 @default.
• W2897545321 cites W2003264965 @default.
• W2897545321 cites W2008374869 @default.
• W2897545321 cites W2010486678 @default.
• W2897545321 cites W2019928317 @default.
• W2897545321 cites W2020585064 @default.
• W2897545321 cites W2024276071 @default.
• W2897545321 cites W2033300742 @default.
• W2897545321 cites W2038206806 @default.
• W2897545321 cites W2039172942 @default.
• W2897545321 cites W2039298735 @default.
• W2897545321 cites W2039589537 @default.
• W2897545321 cites W2042556780 @default.
• W2897545321 cites W2046872014 @default.
• W2897545321 cites W2048053168 @default.
• W2897545321 cites W2050237494 @default.
• W2897545321 cites W2053798956 @default.
• W2897545321 cites W2054630268 @default.
• W2897545321 cites W2055763886 @default.
• W2897545321 cites W2056364365 @default.
• W2897545321 cites W2068054277 @default.
• W2897545321 cites W2073424121 @default.
• W2897545321 cites W2080430451 @default.
• W2897545321 cites W2081550480 @default.
• W2897545321 cites W2085775688 @default.
• W2897545321 cites W2086872426 @default.
• W2897545321 cites W2087179902 @default.
• W2897545321 cites W2087861952 @default.
• W2897545321 cites W2096307493 @default.
• W2897545321 cites W2098010219 @default.
• W2897545321 cites W2101243726 @default.
• W2897545321 cites W2101928296 @default.
• W2897545321 cites W2104447131 @default.
• W2897545321 cites W2105843214 @default.
• W2897545321 cites W2111591597 @default.
• W2897545321 cites W2111675378 @default.
• W2897545321 cites W2121185475 @default.
• W2897545321 cites W2121977390 @default.
• W2897545321 cites W2124224077 @default.
• W2897545321 cites W2134496526 @default.
• W2897545321 cites W2137253883 @default.
• W2897545321 cites W2150827129 @default.
• W2897545321 cites W2154912658 @default.
• W2897545321 cites W2158549296 @default.
• W2897545321 cites W2160938780 @default.
• W2897545321 cites W2161856905 @default.
• W2897545321 cites W2163984817 @default.
• W2897545321 cites W2171095473 @default.
• W2897545321 cites W2176078674 @default.
• W2897545321 cites W2260872739 @default.
• W2897545321 cites W2324917897 @default.
• W2897545321 cites W2368380279 @default.
• W2897545321 cites W2398878895 @default.
• W2897545321 cites W2399007766 @default.
• W2897545321 cites W2461651190 @default.
• W2897545321 cites W2478743214 @default.
• W2897545321 cites W2508674883 @default.
• W2897545321 cites W2522390632 @default.
• W2897545321 cites W2559466477 @default.
• W2897545321 cites W2766971883 @default.
• W2897545321 cites W2789422799 @default.
• W2897545321 doi "https://doi.org/10.2147/copd.s166284" @default.
• W2897545321 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6190813" @default.
• W2897545321 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30349226" @default.
• W2897545321 hasPublicationYear "2018" @default.
• W2897545321 type Work @default.
• W2897545321 sameAs 2897545321 @default.
• W2897545321 citedByCount "14" @default.
• W2897545321 countsByYear W28975453212019 @default.
• W2897545321 countsByYear W28975453212020 @default.
• W2897545321 countsByYear W28975453212021 @default.
• W2897545321 countsByYear W28975453212022 @default.
• W2897545321 countsByYear W28975453212023 @default.

• next