Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897547085> ?p ?o ?g. }
- W2897547085 endingPage "442" @default.
- W2897547085 startingPage "434" @default.
- W2897547085 abstract "The aim of the study was to investigate the role of dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) in intestinal epithelial cells (IECs) in regulating sepsis-induced acute intestinal injury and systemic inflammatory response.To induce sepsis condition, Male C57BL/6 mice were exposed to cecal ligation and puncture (CLP) in vivo, whereas a normal human IECs line (FHs74Int) was stimulated with lipopolysaccharide (LPS) in vitro. DC-SIGN siRNA pretreatment was used to knock down DC-SIGN expression both in vivo and in vitro. The expression of DC-SIGN was detected by western blot and immunohistochemistry. The expression of total and phosphorylation of ERK1/2 and NF-κB/p65 was examined by western blot. The levels of cytokines in serum and culture supernatant were measured by ELISA. The survival rate and organ injures of septic mice were also assessed.In vivo, DC-SIGN expression in mouse IECs was time-dependently upregulated by CLP. CLP-induced phosphorylation of ERK1/2 and NF-κB/p65 was effectively inhibited by DC-SIGN siRNA pretreatment, leading to the decrease of systemic inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-10, and IFN-γ), which alleviated multiple organ injuries and increased the survival rate of septic mice. In vitro, DC-SIGN expression in FHs74Int was significantly upregulated by LPS stimulation in a time- and dose-dependent manner. DC-SIGN knockdown abolished LPS-induced ERK1/2 and NF-κB/p65 phosphorylation, resulting in the decrease of cytokines release by FHs74Int.Sepsis-induced DC-SIGN expression in IECs plays a significant role in regulating acute intestinal injury and systemic inflammatory response. The inhibition of DC-SIGN exhibited protective effects on sepsis-associated organ injury and systemic inflammation." @default.
- W2897547085 created "2018-10-26" @default.
- W2897547085 creator A5001106455 @default.
- W2897547085 creator A5022499603 @default.
- W2897547085 creator A5029829921 @default.
- W2897547085 creator A5030866090 @default.
- W2897547085 creator A5034083344 @default.
- W2897547085 creator A5034458392 @default.
- W2897547085 creator A5037526129 @default.
- W2897547085 creator A5040558103 @default.
- W2897547085 creator A5053804205 @default.
- W2897547085 creator A5057092809 @default.
- W2897547085 creator A5087690220 @default.
- W2897547085 creator A5088965321 @default.
- W2897547085 date "2019-10-01" @default.
- W2897547085 modified "2023-09-24" @default.
- W2897547085 title "DC-SIGN Expression in Intestinal Epithelial Cells Regulates Sepsis-Associated Acute Intestinal Injury Via Activating ERK1/2-NF-κB/P65 Signaling" @default.
- W2897547085 cites W1835356979 @default.
- W2897547085 cites W1944352258 @default.
- W2897547085 cites W1991599442 @default.
- W2897547085 cites W2012865201 @default.
- W2897547085 cites W2023945291 @default.
- W2897547085 cites W2034372373 @default.
- W2897547085 cites W2044471952 @default.
- W2897547085 cites W2048130517 @default.
- W2897547085 cites W2064600163 @default.
- W2897547085 cites W2069120393 @default.
- W2897547085 cites W2070225034 @default.
- W2897547085 cites W2092760439 @default.
- W2897547085 cites W2094173335 @default.
- W2897547085 cites W2106032198 @default.
- W2897547085 cites W2118790372 @default.
- W2897547085 cites W2169109442 @default.
- W2897547085 cites W2227507737 @default.
- W2897547085 cites W2276297238 @default.
- W2897547085 cites W2280404143 @default.
- W2897547085 cites W2291583417 @default.
- W2897547085 cites W2295213040 @default.
- W2897547085 cites W2320994793 @default.
- W2897547085 cites W2335559847 @default.
- W2897547085 cites W2510240660 @default.
- W2897547085 cites W2755199873 @default.
- W2897547085 cites W561293980 @default.
- W2897547085 doi "https://doi.org/10.1097/shk.0000000000001277" @default.
- W2897547085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/30335674" @default.
- W2897547085 hasPublicationYear "2019" @default.
- W2897547085 type Work @default.
- W2897547085 sameAs 2897547085 @default.
- W2897547085 citedByCount "7" @default.
- W2897547085 countsByYear W28975470852019 @default.
- W2897547085 countsByYear W28975470852020 @default.
- W2897547085 countsByYear W28975470852021 @default.
- W2897547085 countsByYear W28975470852022 @default.
- W2897547085 crossrefType "journal-article" @default.
- W2897547085 hasAuthorship W2897547085A5001106455 @default.
- W2897547085 hasAuthorship W2897547085A5022499603 @default.
- W2897547085 hasAuthorship W2897547085A5029829921 @default.
- W2897547085 hasAuthorship W2897547085A5030866090 @default.
- W2897547085 hasAuthorship W2897547085A5034083344 @default.
- W2897547085 hasAuthorship W2897547085A5034458392 @default.
- W2897547085 hasAuthorship W2897547085A5037526129 @default.
- W2897547085 hasAuthorship W2897547085A5040558103 @default.
- W2897547085 hasAuthorship W2897547085A5053804205 @default.
- W2897547085 hasAuthorship W2897547085A5057092809 @default.
- W2897547085 hasAuthorship W2897547085A5087690220 @default.
- W2897547085 hasAuthorship W2897547085A5088965321 @default.
- W2897547085 hasConcept C104317684 @default.
- W2897547085 hasConcept C11960822 @default.
- W2897547085 hasConcept C127561419 @default.
- W2897547085 hasConcept C13373296 @default.
- W2897547085 hasConcept C150903083 @default.
- W2897547085 hasConcept C173396325 @default.
- W2897547085 hasConcept C17991360 @default.
- W2897547085 hasConcept C185592680 @default.
- W2897547085 hasConcept C193976658 @default.
- W2897547085 hasConcept C203014093 @default.
- W2897547085 hasConcept C207001950 @default.
- W2897547085 hasConcept C26291073 @default.
- W2897547085 hasConcept C2776415932 @default.
- W2897547085 hasConcept C2776914184 @default.
- W2897547085 hasConcept C2777730290 @default.
- W2897547085 hasConcept C2778170410 @default.
- W2897547085 hasConcept C2778384902 @default.
- W2897547085 hasConcept C2778754761 @default.
- W2897547085 hasConcept C54355233 @default.
- W2897547085 hasConcept C55493867 @default.
- W2897547085 hasConcept C71924100 @default.
- W2897547085 hasConcept C81885089 @default.
- W2897547085 hasConcept C86803240 @default.
- W2897547085 hasConcept C8891405 @default.
- W2897547085 hasConcept C95444343 @default.
- W2897547085 hasConceptScore W2897547085C104317684 @default.
- W2897547085 hasConceptScore W2897547085C11960822 @default.
- W2897547085 hasConceptScore W2897547085C127561419 @default.
- W2897547085 hasConceptScore W2897547085C13373296 @default.
- W2897547085 hasConceptScore W2897547085C150903083 @default.
- W2897547085 hasConceptScore W2897547085C173396325 @default.
- W2897547085 hasConceptScore W2897547085C17991360 @default.