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- W2897564886 abstract "SNARE complexes assembled between fusing membranes (in trans) are the core machinery driving lipid bilayer merger. Thus, an assay monitoring the formation of these trans-SNARE complexes is essential for SNARE-mediated membrane fusion studies. Homotypic yeast vacuole fusion is an important model system for such studies. Although several assays measuring trans-SNARE complex formation are available to study yeast vacuole fusion, most use SNAREs conjugated with epitope tags, which may affect the function of SNAREs or even the formation of trans-SNARE complexes. Here, I describe an assay for trans-SNARE complex formation during yeast vacuole fusion that does not require epitope-tagged SNAREs." @default.
- W2897564886 created "2018-10-26" @default.
- W2897564886 creator A5010846406 @default.
- W2897564886 date "2018-10-14" @default.
- W2897564886 modified "2023-10-01" @default.
- W2897564886 title "An In Vitro Assay of Trans-SNARE Complex Formation During Yeast Vacuole Fusion Using Epitope Tag-Free SNAREs" @default.
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- W2897564886 doi "https://doi.org/10.1007/978-1-4939-8760-3_18" @default.
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