FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2897569666> ?p ?o ?g. }

• W2897569666 endingPage "302" @default.
• W2897569666 startingPage "293" @default.
• W2897569666 abstract "There is an urgent need for development of rapid and inexpensive techniques for detection of microRNAs (miRNAs), which are potential biomarkers of various types of cancer. In this paper, we describe a multiplexed electrochemical platform for determination of three cancer-relevant miRNAs: miR-21, let-7a and miR-31. The strategy combines the use of magnetic beads (MBs) modified with a commercial antibody for the efficient capture of the heteroduplexes formed by hybridization of the target miRNA with DNA probe. Free non-hybridized region of the DNA probe was thereafter hybridized with two biotin-labeled auxiliary DNA probes in a process of hybridization chain reaction (HCR), resulting in a long hybrid bearing a large number of biotin molecules. Labeling of these multiple biotin units with streptavidin-peroxidase conjugates allowed an amplification of the amperometric signal measured after capturing the modified MBs at a screen-printed carbon electrode array of eight electrodes. The combined strategy demonstrated in a similar assay time significantly higher sensitivity than those previously described using modified MBs with the same capture antibody (without amplification by HCR) or a HCR strategy implemented on the surface of MBs, respectively. The methodology exhibits a good selectivity for discriminating single mismatches and was applied to the determination of the three target miRNAs in total RNA (RNAt) extracted from various cancer cell lines and from cervical precancerous lesions." @default.
• W2897569666 created "2018-10-26" @default.
• W2897569666 creator A5012253323 @default.
• W2897569666 creator A5034601455 @default.
• W2897569666 creator A5039506098 @default.
• W2897569666 creator A5059521086 @default.
• W2897569666 creator A5084695348 @default.
• W2897569666 date "2018-10-22" @default.
• W2897569666 modified "2023-10-16" @default.
• W2897569666 title "Multiplexed Immunosensing Platform Coupled to Hybridization Chain Reaction for Electrochemical Determination of MicroRNAs in Clinical Samples" @default.
• W2897569666 cites W1520719781 @default.
• W2897569666 cites W1672385484 @default.
• W2897569666 cites W1680239125 @default.
• W2897569666 cites W1994629806 @default.
• W2897569666 cites W2004447128 @default.
• W2897569666 cites W2005440577 @default.
• W2897569666 cites W2017362104 @default.
• W2897569666 cites W2017677233 @default.
• W2897569666 cites W2036363466 @default.
• W2897569666 cites W2040279072 @default.
• W2897569666 cites W2056113491 @default.
• W2897569666 cites W2071229287 @default.
• W2897569666 cites W2071353826 @default.
• W2897569666 cites W2084078092 @default.
• W2897569666 cites W2086020274 @default.
• W2897569666 cites W2087604517 @default.
• W2897569666 cites W2096573862 @default.
• W2897569666 cites W2100494614 @default.
• W2897569666 cites W2108476208 @default.
• W2897569666 cites W2118030566 @default.
• W2897569666 cites W2144149602 @default.
• W2897569666 cites W2146571198 @default.
• W2897569666 cites W2151501050 @default.
• W2897569666 cites W2153780208 @default.
• W2897569666 cites W2255775521 @default.
• W2897569666 cites W2317304611 @default.
• W2897569666 cites W2336431641 @default.
• W2897569666 cites W2415231114 @default.
• W2897569666 cites W2465572659 @default.
• W2897569666 cites W2520824122 @default.
• W2897569666 cites W2604185487 @default.
• W2897569666 cites W2620625960 @default.
• W2897569666 cites W2623712937 @default.
• W2897569666 cites W2730989166 @default.
• W2897569666 cites W2749318227 @default.
• W2897569666 cites W2767350461 @default.
• W2897569666 doi "https://doi.org/10.1002/elan.201800573" @default.
• W2897569666 hasPublicationYear "2018" @default.
• W2897569666 type Work @default.
• W2897569666 sameAs 2897569666 @default.
• W2897569666 citedByCount "23" @default.
• W2897569666 countsByYear W28975696662019 @default.
• W2897569666 countsByYear W28975696662020 @default.
• W2897569666 countsByYear W28975696662021 @default.
• W2897569666 countsByYear W28975696662022 @default.
• W2897569666 countsByYear W28975696662023 @default.
• W2897569666 crossrefType "journal-article" @default.
• W2897569666 hasAuthorship W2897569666A5012253323 @default.
• W2897569666 hasAuthorship W2897569666A5034601455 @default.
• W2897569666 hasAuthorship W2897569666A5039506098 @default.
• W2897569666 hasAuthorship W2897569666A5059521086 @default.
• W2897569666 hasAuthorship W2897569666A5084695348 @default.
• W2897569666 hasConcept C129312508 @default.
• W2897569666 hasConcept C130197536 @default.
• W2897569666 hasConcept C137390916 @default.
• W2897569666 hasConcept C153911025 @default.
• W2897569666 hasConcept C159654299 @default.
• W2897569666 hasConcept C185592680 @default.
• W2897569666 hasConcept C2776190903 @default.
• W2897569666 hasConcept C2778204606 @default.
• W2897569666 hasConcept C2780420688 @default.
• W2897569666 hasConcept C54355233 @default.
• W2897569666 hasConcept C552990157 @default.
• W2897569666 hasConcept C55493867 @default.
• W2897569666 hasConcept C86803240 @default.
• W2897569666 hasConceptScore W2897569666C129312508 @default.
• W2897569666 hasConceptScore W2897569666C130197536 @default.
• W2897569666 hasConceptScore W2897569666C137390916 @default.
• W2897569666 hasConceptScore W2897569666C153911025 @default.
• W2897569666 hasConceptScore W2897569666C159654299 @default.
• W2897569666 hasConceptScore W2897569666C185592680 @default.
• W2897569666 hasConceptScore W2897569666C2776190903 @default.
• W2897569666 hasConceptScore W2897569666C2778204606 @default.
• W2897569666 hasConceptScore W2897569666C2780420688 @default.
• W2897569666 hasConceptScore W2897569666C54355233 @default.
• W2897569666 hasConceptScore W2897569666C552990157 @default.
• W2897569666 hasConceptScore W2897569666C55493867 @default.
• W2897569666 hasConceptScore W2897569666C86803240 @default.
• W2897569666 hasFunder F4320321006 @default.
• W2897569666 hasFunder F4320321837 @default.
• W2897569666 hasIssue "2" @default.
• W2897569666 hasLocation W28975696661 @default.
• W2897569666 hasOpenAccess W2897569666 @default.
• W2897569666 hasPrimaryLocation W28975696661 @default.
• W2897569666 hasRelatedWork W1970008822 @default.
• W2897569666 hasRelatedWork W1995511477 @default.
• W2897569666 hasRelatedWork W2002724621 @default.
• W2897569666 hasRelatedWork W2013018133 @default.

• next