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- W2897576147 abstract "Introduction: Luxol fast blue (LFB) is a dye that binds stoichiometrically to myelin and allows quantification of the myelination in preparations from the CNS. Therefore, LFB`s can be used to evaluate the damage after spinal cord injury (SCI) and the effect of diverse treatment methods on remyelination in the injured spinal cord. Materials and methods: 37 female Wistar rats were subjected to a compressive SCI at a low-thoracic level (vertebra Th8). The animals were randomized to four groups treated daily with whole body vibration (WBV) starting 1, 7, 14 or 28 days after injury (WBV1, WBV7, WBV14, WBV28) that continued for 12-weeks. Four groups served as controls - rats euthanized in the first 2 weeks post SCI (subacute), untreated (Sham therapy), treated with passive flexion-extension (PFE) and intact animals. We measured the optical density (OD) of longitudinal spinal cord sections stained with LFB. The entire slice area and a region of interest spanning 2.5 mm on both sides of the compression site were evaluated. In a second step we applied an image mask to exclude the `empty` fields from the section (due to vessels, tears or damage vacuoles). Thus, the OD is evaluated once in the entire sectional plane and a second time only in the preserved tissues (correspondingly the preserved neural tissue bridging the compression (PNTB)). The two measurement modes allowed the calculation of different quantitative features of the LFB staining. Results: WBV groups showed different degree of remyelination compared to sham therapy. A trend for better (re)myelination in the PNTB was observed in the WBV14 group. Conclusion: Using LFB staining for analysis in SCI animal models is suitable for quantification of the myelination. The method allows an evaluation and comparison between the different groups and treatment options. WBV could enhance remyelination depending on the day it was initiated." @default.
- W2897576147 created "2018-10-26" @default.
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- W2897576147 date "2018-04-12" @default.
- W2897576147 modified "2023-09-25" @default.
- W2897576147 title "Method for quantification of the myelin content using luxol fast blue staining in injured spinal cord of adult rats and whole body vibration as a possible method for enhancing (re)myelination" @default.
- W2897576147 doi "https://doi.org/10.14748/ssvs.v2i0.4714" @default.
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